Myeloperoxidase/MPO Antibody

Western Blot: Myeloperoxidase/MPO Antibody [NB600-923] - Detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.

Product Discontinued

Myeloperoxidase/MPO Antibody Summary

• Description: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Myeloperoxidase/MPO/MPO [Human Leukocytes] (Uniprot: P05164)
• Localization: Lysosomal
• Specificity: Cross reactivity against Myeloperoxidase/MPO from other tissues and species may occur but have not been specifically determined.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MPO
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:200000
  • Immunoprecipitation 1:100
  • Western Blot 1:5000
• Application Notes: This product has been tested in western blotting. This antibody is suitable for ELISA and immunoprecipitation. The antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combination (68 kDa and 106 kDa). Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. Anti-Human Myeloperoxidase may react with MPO from other sources. Anti-Human Myeloperoxidase detects neutrophilic granulocytes and monocytes in blood and precursors of granulocytes in the bone marrow. This antibody may also detect myeloid leukemias of the bone marrow as well as other sites.

Reactivity Notes

Cross reactivity against Myeloperoxidase/MPO from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for Myeloperoxidase/MPO Antibody

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloperoxidase/MPO Antibody (NB600-923)(1)

Publication using NB600-923

• Murphy Mp, Mcenery T, Mcquillan K Et Al. Alpha-1 antitrypsin therapy modulates the neutrophil membrane proteome and secretome Eur. Respir. J. 2020-02-14 [PMID: 32060059]

Bioinformatics

• Gene Symbol: MPO
• Entrez:
  • 4353(Human)
• Uniprot:
  • P05164()
  • P05164()
  • P05164()
  • P05164()