Myeloperoxidase/MPO Antibody (3E11)

Western Blot: Myeloperoxidase/MPO Antibody (3E11) [H00004353-M01] - MPO monoclonal antibody (M01), clone 3E11. Analysis of MPO expression in Jurkat.

Western Blot: Myeloperoxidase/MPO Antibody (3E11) [H00004353-M01] - MPO monoclonal antibody (M01), clone 3E11. Analysis of MPO expression in Raw 264.7.

ELISA: Myeloperoxidase/MPO Antibody (3E11) [H00004353-M01] - Myeloperoxidase/MPO Antibody (3E11) [H00004353-M01] - Detection limit for recombinant GST tagged MPO is 0.3 ng/ml as a capture antibody.

Product Discontinued

Myeloperoxidase/MPO Antibody (3E11) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MPO (NP_000241, 646 a.a. ~ 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWENEGVFSMQQRQALAQISLPRIICDNTGITTVSKNNIFMSNSYPRDFVNCSTLPALNLASWREAS
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPO
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: It has been used for ELISA and WB.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Myeloperoxidase/MPO Antibody (3E11)

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Myeloperoxidase/MPO Antibody (H00004353-M01). (Showing 1 - 1 of 1 FAQ).

1. Our laboratory would like to assess the extent of leukocyte infiltration into rabbit kidney induced by metabolic acidosis. We are currently exploring the feasibility of a myeloperoxidase assay utilizing antibody capture of myeloperoxidase for this purpose. Rabbit myeloperoxidase shares 88% and 86% identity with the human and mouse orthologues, respectively. Can you determine based on the immunogen, antibody epitope, or from direct testing data whether this antibody will cross-react with the rabbit orthologue?
   • Unfortunately, we have not mapped the actual epitope nor have we tested directly in rabbit. Based on the immunogen pf 646-743 aa. homology to rabbit we would say that there is definite cross reactivity with 85% homology. VSEP LKRKGRVGPL LACIIGTQFR KLRDGDRFWW ENEGVFSMQQ RQALAQISLP RIICDNTGIT TVSKNNIFMS NSYPRDFVNC STLPALNLAS WREAS I did check the other MPO antibodies and the problem is we do not have the exact epitope for any and when they use the full length for immunization then we cannot say for certain. However, with full length homology being 88% from human to rabbit is no good region of exclusivity.

Bioinformatics

• Gene Symbol: MPO
• Uniprot:
  • NP_000241(Human)