MYB Antibody [Unconjugated]

Western blot shows lysates of CEM human T-lymphoblastoid cell line, SW620 human colorectal adenocarcinoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse MYB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6209) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MYB at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

MYB Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MYB
  Lys503-Met640
  Accession # P10242
• Specificity: Detects human and mouse MYB in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: MYB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MYB Antibody [Unconjugated]

• Cmyb;c-myb;c-myb_CDS;efg;Transcriptional activator Myb
• MYB

Background

MYB (myeloblastosis protooncogene) is an 80 kDa transcriptional activator. It is found in hematopoietic and intestinal crypt epithelial cells. MYB interacts with p300 and activates multiple genes, including Bcl2, GATA3, and MYC. Although MYB is thought of as a pro-proliferation and antidifferentiation factor, it would now appear that MYB contributes to both T- and B-cell maturation and to cell survival in general. Human MYB is 640 amino acids (aa) in length. It contains three DNA binding, HTH‑myb type domains (aa 35‑193), one transcriptional activation segment (aa 275‑327), and a Leu‑zipper motif (aa 376‑397). There are multiple potential splice forms. One shows an alternate start site at Met189 coupled with a 99 aa substitution for aa 567‑640. Another shows a 121 aa insertion after Ser401. Four show C‑terminal substitutions: a 34 aa substitution for aa 314‑640, a Met substitution for aa 402‑640, a seven aa substitution for aa 317‑640, and a 10 aa substitution for aa 603‑640. Over aa 503‑640, human MYB shares 84% aa identity with mouse MYB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYB Antibody (AF6209)(1)

Publication using AF6209

• Mélanie A. Cron, Solène Maillard, Frédérique Truffault, Ambra Vittoria Gualeni, Annunziata Gloghini, Elie Fadel et al. Causes and Consequences of miR-150-5p Dysregulation in Myasthenia Gravis Frontiers in Immunology 2019-03-29 [PMID: 30984166]

Bioinformatics

• Gene Symbol: MYB
• Uniprot:
  • P10242(Human)