MYB Antibody [Alexa Fluor® 532]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

MYB Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human MYB
  Lys503-Met640
  Accession # P10242
• Specificity: Detects human and mouse MYB in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MYB Antibody [Alexa Fluor® 532]

• Cmyb;c-myb;c-myb_CDS;efg;Transcriptional activator Myb
• MYB

Background

MYB (myeloblastosis protooncogene) is an 80 kDa transcriptional activator. It is found in hematopoietic and intestinal crypt epithelial cells. MYB interacts with p300 and activates multiple genes, including Bcl2, GATA3, and MYC. Although MYB is thought of as a pro-proliferation and antidifferentiation factor, it would now appear that MYB contributes to both T- and B-cell maturation and to cell survival in general. Human MYB is 640 amino acids (aa) in length. It contains three DNA binding, HTH‑myb type domains (aa 35‑193), one transcriptional activation segment (aa 275‑327), and a Leu‑zipper motif (aa 376‑397). There are multiple potential splice forms. One shows an alternate start site at Met189 coupled with a 99 aa substitution for aa 567‑640. Another shows a 121 aa insertion after Ser401. Four show C‑terminal substitutions: a 34 aa substitution for aa 314‑640, a Met substitution for aa 402‑640, a seven aa substitution for aa 317‑640, and a 10 aa substitution for aa 603‑640. Over aa 503‑640, human MYB shares 84% aa identity with mouse MYB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.