Muscle Phosphofructokinase/PFKM/PFK-1 Antibody

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Biological Strategies: Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.
Immunoprecipitation: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate 293T cells.

Product Details

Summary
Reactivity Hu, Mu, Ca, EqSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Muscle Phosphofructokinase/PFKM/PFK-1 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Phosphofructokinase, Muscle using the numbering given in entry NP_000280.1 (GeneID 5213).
Predicted Species
Canine (100%), Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PFKM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Reviewed Applications
Read 1 Review rated 5
using
NBP2-32169 in the following applications:

Publications
Read Publication using
NBP2-32169 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody

  • 6-phosphofructokinase, muscle type
  • EC 2.7.1,6-phosphofructo-1-kinase
  • EC 2.7.1.11
  • GSD7
  • MGC8699
  • Muscle Phosphofructokinase
  • PFK1
  • PFK-1
  • PFKA
  • PFK-A
  • PFKM
  • PFKX
  • Phosphofructo-1-kinase isozyme A
  • Phosphofructokinase 1
  • phosphofructokinase, muscle
  • phosphofructokinase, polypeptide X
  • Phosphofructokinase-1
  • Phosphofructokinase-M
  • Phosphohexokinase

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (NBP2-32169)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF/IHC.


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Review for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (NBP2-32169) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-32169:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Muscle Phosphofructokinase/PFKM/PFK-1 NBP2-32169
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5
reviewed by:
Verified Customer
WB Mouse 11/12/2015
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Summary

ApplicationWestern Blot
Sample Testedmouse liver
SpeciesMouse
LotA1

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Recent Reviews

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Verified Customer
11/12/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PFKM
Uniprot