PFKFB2 Antibody

Immunohistochemistry-Paraffin: PFKFB2 Antibody [NBP1-45238] - 2.5ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PFKFB2 Antibody Summary

• Immunogen: Peptide with sequence SEQNNNSYETKTP-C corresponding to N-Terminus according to NP_006203.2, NP_001018063.1.
• Epitope: N-terminus
• Specificity: This antibody is expected to recognize both reported isoforms (NP_006203.2; NP_001018063.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PFKFB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot detection limit 1:16000
• Application Notes: Western blot: Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5kDa according to NP_006203.2 and 54.4kDa according to NP_001018063.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide
  IHC: Paraffin embedded Human Spleen.

Reactivity Notes

Expected from sequence similarity: Canine, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PFKFB2 Antibody

• 6PF-2-K/Fru-2,6-P2ase 2
• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
• DKFZp781D2217
• fructose-2,6-bisphosphatase, cardiac isozyme
• MGC138308,6PF-2-K/Fru-2,6-P2ase heart-type isozyme
• MGC138310
• PFK/FBPase 2
• PFK-2/FBPase-2
• PFKFB, cardiac

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include, PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PFKFB2 Antibody (NBP1-45238)(1)

Publication using NBP1-45238

• Arden C, Hampson LJ, Huang GC et al. A role for PFK-2/FBPase-2, as distinct from fructose 2,6-bisphosphate, in regulation of insulin secretion in pancreatic beta-cells. Biochem J 2008-04-01 [PMID: 18039179]

Bioinformatics

• Gene Symbol: PFKFB2
• Entrez:
  • 5208(Human)
• Uniprot:
  • NP_006203.2(Human)