HAF018). A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Muscle Phospho-fructokinase/PFKM Monoclonal Antibody (Catalog # MAB7687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7687-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87293-25ul | |||
HAF016). A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human aorta tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Muscle Phosphofructokinase/PFKM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7687) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7687-SP | |||
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Western blot analysis of Muscle Phosphofructokinase/PFKM/PFK-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: PC-3M cell lysate](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Western-Blot-NBP2-75578-img0011.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Western blot analysis of Muscle Phosphofructokinase/PFKM/PFK-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: PC-3M cell lysate")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunocytochemistry-Immunofluorescence-NBP2-75578-img0004.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded rat brain tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0009.jpg)
![Flow Cytometry: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of SH-SY-5Y cells with Fructose 6 Phosphate Kinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Flow-Cytometry-NBP2-75578-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunocytochemistry-Immunofluorescence-NBP2-75578-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunocytochemistry-Immunofluorescence-NBP2-75578-img0003.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded human liver cancer tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0005.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded human thyroid gland tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0006.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded human kidney tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0007.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0008.jpg)
![Western blot analysis on different lysates with NBP2-75578 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Ramos cell lysate Lane 3: Mouse heart tissue lysate Lane 4: Mouse skeletal muscle tissue lysate Lane 5: Rat heart tissue lysate Lane 6: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 75 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-75578 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75578_-western-blot-639204586672635492.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32169-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate 293T cells.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunoprecipitation-NBP2-32169-img0003.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0012.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87293-img0013.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87293-img0010.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0011.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249432858.jpg "Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-21202494149.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-212024941459.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Wild-type (WT) and PFKM knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249422638.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Muscle Phosphofructokinase/PFKM/PFK-1 antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-western-blot-1411202518312123.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Analysis demonstrates the labeling specificity of the antibodies used for Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given. Used with permission from Vallejo and Hardin (2004).](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-44228-img0003.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Analysis demonstrates the labeling specificity of the antibodies used for Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given. Used with permission from Vallejo and Hardin (2004).")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Cultures of A7r5 VSM cells (rat aorta) were labeled with antibody and anti-caveolin (CAV)-1. Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell. Used with permission from Vallejo and Hardin (2004).](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44228-img0001.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFK-1, NBP2-32170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32170-img0003.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFK-1, NBP2-32170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for WB at 0.1ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32170-img0004.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013] - Western blot detection of Muscle Phosphofructokinase/PFKM/PFK-1 in C6, 3T3, Hela cell lysates using NBP3-20013 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-S08-6B4-Western-Blot-NBP3-20013-img0002.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013] - Western blot detection of Muscle Phosphofructokinase/PFKM/PFK-1 in C6, 3T3, Hela cell lysates using NBP3-20013 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013] - Immunofluorescence of Muscle Phosphofructokinase/PFKM/PFK-1 (green) in Hela cells using NBP3-20013 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-20013_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-s08-6b4-113202515145460.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (4Z7X1) [NBP3-16242] - Western blot analysis of extracts of various cell lines, using Muscle Phosphofructokinase/PFKM/PFK-1 Rabbit mAb (NBP3-16242) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-4Z7X1-Western-Blot-NBP3-16242-img0001.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (4Z7X1) [NBP3-16242] - Western blot analysis of extracts of various cell lines, using Muscle Phosphofructokinase/PFKM/PFK-1 Rabbit mAb (NBP3-16242) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (4Z7X1) [NBP3-16242] - Western blot analysis of extracts of various cell lines, using Muscle Phosphofructokinase/PFKM/PFK-1 Rabbit mAb (NBP3-16242) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-4Z7X1-Western-Blot-NBP3-16242-img0002.jpg)
![Western Blot: PFK-1 Overexpression Lysate (Adult Normal) [NBL1-14316] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PFK-1.](http://images.novusbio.com/fullsize/PFK-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14316-img0002.jpg "Western Blot: PFK-1 Overexpression Lysate (Adult Normal) [NBL1-14316] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PFK-1.")
