HAF019). A specific band was detected for MuRF1 at approximately 41-44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human heart, mouse heart, and rat muscle tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for MuRF1 at approximately 41-44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5366-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52516-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52515-0.025mg | |||
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-2406] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-NB100-2406-img0001.jpg "Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-2406] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: MuRF1/TRIM63 Antibody [NBP1-54939] - THP-1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-Western-Blot-NBP1-54939-img0003.jpg "Western Blot: MuRF1/TRIM63 Antibody [NBP1-54939] - THP-1 cell lysate, concentration 0.2-1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of Hela cells using MuRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-7B8E1-Immunofluorescence-NBP2-52516-img0002.jpg "Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of Hela cells using MuRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.")
![Immunohistochemistry: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of paraffin-embedded rectum cancer tissues using MuRF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-7B8E1-Immunohistochemistry-NBP2-52516-img0003.jpg)
![Flow Cytometry: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of Hela cells using MuRF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-7B8E1-Flow-Cytometry-NBP2-52516-img0001.jpg)
![Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of paraffin-embedded muscle tissues using MuRF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-7B8E1-Immunohistochemistry-Paraffin-NBP2-52516-img0004.jpg)
![ELISA: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-7B8E1-ELISA-NBP2-52516-img0006.jpg)
![Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of TRIM63 expression in transfected 293T cell line by TRIM63 monoclonal antibody (M01), clone 6G6. Lane 1: TRIM63 transfected lysate(40.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-6G6-Western-Blot-H00084676-M01-img0009.jpg "Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of TRIM63 expression in transfected 293T cell line by TRIM63 monoclonal antibody (M01), clone 6G6. Lane 1: TRIM63 transfected lysate(40.2 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of monoclonal antibody to TRIM63 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-6G6-Immunocytochemistry-Immunofluorescence-H00084676-M01-img0006.jpg)
![Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of TRIM63 over-expressed 293 cell line, cotransfected with TRIM63 Validated Chimera RNAi ( Cat # H00084676-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM63 monoclonal antibody (M01), clone 6G6 (Cat # H00084676-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-6G6-Western-Blot-H00084676-M01-img0007.jpg)
![Sandwich ELISA: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Detection limit for recombinant GST tagged TRIM63 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-6G6-Sandwich-ELISA-H00084676-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-1193] - (1.25ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-Immunohistochemistry-Paraffin-NB100-1193-img0002.jpg "Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-1193] - (1.25ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody [NBP3-23425] - Immunocytochemical labeling of MuRF1/TRIM63 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1/TRIM63 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.](http://images.novusbio.com/fullsize/nbp3-23425_rabbit-murf1-trim63-pab-19920231410120.jpg "Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody [NBP3-23425] - Immunocytochemical labeling of MuRF1/TRIM63 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1/TRIM63 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.")
![Western Blot: MuRF1/TRIM63 Antibody [NBP3-23425] - Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1/TRIM63 (C-terminal region) (lanes 1 & 2) or anti-Fbx32 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23425_rabbit-murf1-trim63-pab-19920231410118.jpg)
![Western Blot: MURF1 Antibody [NBP2-11061] - WB of antibody with sample. Antibody dilution 1:500 in Dilution Buffer. MW of MURF1 is 41 and 52 kDa.](http://images.novusbio.com/fullsize/MURF1-Antibody-Western-Blot-NBP2-11061-img0001.jpg "Western Blot: MURF1 Antibody [NBP2-11061] - WB of antibody with sample. Antibody dilution 1:500 in Dilution Buffer. MW of MURF1 is 41 and 52 kDa.")
![Western Blot: MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free [NBP3-25570] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [HL1450] (NBP3-25570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25570_rabbit-murf1-trim63-mab-hl1450-azide-and-bsa-free-2120249413319.jpg "Western Blot: MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free [NBP3-25570] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [HL1450] (NBP3-25570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free [NBP3-25570] - Human tissue extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [HL1450] (NBP3-25570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25570_rabbit-murf1-trim63-mab-hl1450-azide-and-bsa-free-2120249393574.jpg)
![Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody (6H6D4) [NBP2-52515] - Analysis of muscle tissues using MuRF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-6H6D4-Immunohistochemistry-NBP2-52515-img0001.jpg "Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody (6H6D4) [NBP2-52515] - Analysis of muscle tissues using MuRF1 mouse mAb with DAB staining.")
![ELISA: MuRF1/TRIM63 Antibody (6H6D4) [NBP2-52515] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-6H6D4-ELISA-NBP2-52515-img0002.jpg)
