MUC6 Antibody (CLH5)

Images

 
Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374] - Human Gastric Carcinoma stained with MUC6 Monoclonal Antibody (CLH5).
Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC6 Antibody (CLH5). Courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
CLH5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Catalog# & Formulation Size Price

MUC6 Antibody (CLH5) Summary

Immunogen
A synthetic peptide of the Gastric Mucin 6 tandem repeat sequence.
Localization
Cytoplasmic & Secreted
Specificity
The MUC6 gastric mucin is a secreted glycoprotein that plays an essential role in epithelial cyto-protection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 is associated with Lewis type 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
252 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-44374 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC6 Antibody (CLH5)

  • gastric mucin
  • Gastric mucin-6
  • MUC-6
  • mucin 6, gastric
  • mucin 6, oligomeric mucus/gel-forming
  • mucin-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MUC6 Antibody (NBP2-44374)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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IHC
(1)
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(1)
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MUC6