MUC3 Antibody (MUC3/1154)

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434] - Human colorectal cancer tissue stained with MUC3 Antibody (MUC3/1154). IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434] - Human Colon Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434] - Human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434] - Human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).

• Reactivity: Hu, Rt(-)
• Applications: Flow, ICC/IF, IHC, IHC-P, ICC/IF
• Clone: MUC3/1154
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MUC3 Antibody (MUC3/1154) Summary

• Immunogen: A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
• Localization: Cytoplasmic and cell surface
• Specificity: It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This monoclonal antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC3A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 1100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47687)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for MUC3 Antibody (MUC3/1154)

• MUC-3A
• mucin 3, intestinal
• mucin 3A, cell surface associated
• mucin 3A, intestinal
• mucin-3A

Background

The mucin genes encode epithelial glycoproteins, some of which are secreted and some membrane bound. Each of the genes contains at least one large domain of tandemly repeated sequence that encodes the peptide sequence rich in serine and/or threonine residues, which carries most of the O-linked glycosylation (Gendler and Spicer, 1995 (PubMed 7778880)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MUC3 Antibody (NBP2-44434) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Lindsey Johnson IHC-P Human 10/08/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Colorectal cancer
• Species: Human
• Lot: MUC3/1154

Comments

• Comments: PFA fixed paraffin-embedded sections were deparaffinized in serial dilutions of ethanol. 10% serum was used for blocking of non-specific binding for 1 hour. Primary antibody dilution was 1:100.

Bioinformatics

• Gene Symbol: MUC3A
• Uniprot:
  • Q02505(Human)
  • Q02505(Human)
  • Q9H195(Human)