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MUC3 Antibody (MUC3/1154) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Rt(-)Species Glossary
Applications Flow, IHC, IHC-P, ICC/IF
Clone
MUC3/1154
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47687AF350
Alexa Fluor 405
NBP2-47687AF405
Alexa Fluor 488
NBP2-47687AF488
Alexa Fluor 532
NBP2-47687AF532
Alexa Fluor 594
NBP2-47687AF594
Alexa Fluor 647
NBP2-47687AF647
Alexa Fluor 700
NBP2-47687AF700
Alexa Fluor 750
NBP2-47687AF750
Allophycocyanin
NBP2-47687APC
Allophycocyanin/Cy7
NBP2-47687APCCY7
Biotin
NBP2-47687B
DyLight 350
NBP2-47687UV
DyLight 405
NBP2-47687V
DyLight 488
NBP2-47687G
DyLight 550
NBP2-47687R
DyLight 594
NBP2-47687DL594
DyLight 650
NBP2-47687C
DyLight 680
NBP2-47687FR
DyLight 755
NBP2-47687IR
FITC
NBP2-47687F
HRP
NBP2-47687H
Janelia Fluor 646
NBP2-47687JF646
PE
NBP2-47687PE
PE/Atto594
NBP2-47687PEATT594
PE/Cy5.5
NBP2-47687PECY55
PE/Cy7
NBP2-47687PECY7
PerCP
NBP2-47687PCP
Unconjugated
NBP2-44434-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44434-0.1mg
IHC-Prediluted
NBP2-48415
Azide and BSA Free
NBP2-47687-0.1mg
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MUC3 Antibody (MUC3/1154) [Janelia Fluor® 549] Summary

Immunogen
A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
Localization
Cytoplasmic and cell surface
Specificity
It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC3A
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MUC3 Antibody (MUC3/1154) [Janelia Fluor® 549]

  • MUC-3A
  • mucin 3, intestinal
  • mucin 3A, cell surface associated
  • mucin 3A, intestinal
  • mucin-3A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: MUC2 Antibody (996/1) [NB120-11197] - MS-induced intestinal epithelium injury was CRHR1 dependent.Photomicrographs of immunofluorescence of Mucin 2 (Muc2; mucous-forming protein) (F-J) in proximal colon in all experimental groups. Image collected and cropped by CiteAb from the following publication (nature.com/articles/srep46616), licensed under a CC-BY licence.
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Immunocytochemistry/Immunofluorescence: MUC2 Antibody (996/1) [NB120-11197] - MS-induced intestinal epithelium injury was CRHR1 dependent. Photomicrographs of immunofluorescence of Mucin 2 (Muc2; mucous-forming protein) (F-J) in proximal colon in all experimental groups. Image collected and cropped by CiteAb from the following publication (nature.com/articles/srep46616), licensed under a CC-BY licence.
(1 Review)
(4 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
MUC1 Antibody (SM3)
NB120-22711
Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MUC1 [SM3] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.
Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MUC-1 antibody was tested in MCF7 cells at 10 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.
(1 Review)
(11 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MUC4 Antibody
NBP1-52193
Western Blot: MUC4 Antibody [NBP1-52193] - MUC4 is shown in the green channel, with beta-tubulin in red channel and Precision Plus dual color protein ladder in the left. The main band is about 60 kDa, with another band about 40 kDa. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in mouse prostate
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MUC5AC Antibody
(45M1)
NBP2-15196
Western Blot: MUC5AC Antibody (45M1) [NBP2-15196] - AGS human gastric adenocarcinoma and KATO-III human gastric carcinoma cell line lysates. Image from verified customer review.
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).
Flow Cytometry: MUC5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196PE (blue) and a matched isotype control NBP2-27287PE (orange). To block the secretion of Mucin 5AC, cells were treated with 3uM Monensin for 3 hours. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both Antibodies were conjugated to Phycoerythrin.
(4 Reviews)
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Species: Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
MUC5B Antibody
NBP1-92151
Immunocytochemistry/Immunofluorescence: MUC5B Antibody [NBP1-92151] - Staining of human cell line A549 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151] - Analysis in human cervix, uterine and skeletal muscle tissues. Corresponding MUC5B RNA-seq data are presented for the same tissues.
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Applications: ICC/IF, IHC, IHC-P
MUC6 Antibody
(CLH5)
NBP2-44374
Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374] - Human Gastric Carcinoma stained with MUC6 Monoclonal Antibody (CLH5).
Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC6 Antibody (CLH5). Courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty, University of Porto, Portugal
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MUC7 Antibody
NBP1-82045
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MUC12 Antibody
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[Unconjugated]
210-TA
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MUC17 Antibody
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(1 Review)
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CFTR Antibody
(24-1) [Unconjugated] ...
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EGF [Unconjugated]
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1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
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(21 Reviews)
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SLC25A1 Antibody
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Western Blot: SLC25A1 Antibody [NBP1-31851] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A1 antibody [N1C1] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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Western Blot: SLC25A1 Antibody [NBP1-31851] - Rat tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A1 antibody [N1C1] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
(1 Publication)
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Cytokeratin 20
Antibody (SA35-03)
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ErbB2/Her2
[Unconjugated]
1129-ER
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MUC13 Antibody
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Western Blot: MUC13 Antibody [NBP2-24566] - Total protein from human, HeLa, A549, Hek293 and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MUC13 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
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MUC3 Antibody
(MUC3/1154) [Janelia ...
NBP2-47687JF549
MUC3 Antibody (MUC3/1154) [Janelia Fluor® 549]
Species: Hu, Rt(-)
Applications: Flow, IHC, IHC-P, ICC/IF

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47687AF350
Alexa Fluor 405 NBP2-47687AF405
Alexa Fluor 488 NBP2-47687AF488
Alexa Fluor 532 NBP2-47687AF532
Alexa Fluor 594 NBP2-47687AF594
Alexa Fluor 647 NBP2-47687AF647
Alexa Fluor 700 NBP2-47687AF700
Alexa Fluor 750 NBP2-47687AF750
Allophycocyanin NBP2-47687APC
Allophycocyanin/Cy7 NBP2-47687APCCY7
Biotin NBP2-47687B
DyLight 350 NBP2-47687UV
DyLight 405 NBP2-47687V
DyLight 488 NBP2-47687G
DyLight 550 NBP2-47687R
DyLight 594 NBP2-47687DL594
DyLight 650 NBP2-47687C
DyLight 680 NBP2-47687FR
DyLight 755 NBP2-47687IR
FITC NBP2-47687F
HRP NBP2-47687H
Janelia Fluor 549 NBP2-47687JF549
Janelia Fluor 646 NBP2-47687JF646
PE NBP2-47687PE
PE/Atto594 NBP2-47687PEATT594
PE/Cy5.5 NBP2-47687PECY55
PE/Cy7 NBP2-47687PECY7
PerCP NBP2-47687PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol MUC3A