TFF3 Antibody

Images

 
Western Blot: TFF3 Antibody [NBP1-76514] - Analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide
Immunocytochemistry/ Immunofluorescence: TFF3 Antibody [NBP1-76514] - TFF3 in human small intestine tissue with TFF3 antibody at 20 ug/ml.
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP1-76514] - TFF3 in human small intestine tissue with TFF3 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TFF3 Antibody [NBP1-76514] - in Human Small Intestine cells with TFF3 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: TFF3 Antibody [NBP1-76514] - TFF3 in HeLa cells with TFF3 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP1-76514] - in human small intestine tissue with TFF3 antibody at 2.5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TFF3 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3. The immunogen is located within the last 50 amino acids of TFF3.
Specificity
TFF3 antibody is predicted to not cross-react with other TFF family members.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TFF3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5ug/mL
Theoretical MW
10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
TFF3 Peptide (NBP1-76514PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TFF3 Antibody

  • HITF, human intestinal trefoil factor10Intestinal trefoil factor
  • ITF
  • TFF3
  • TFI
  • trefoil factor 3 (intestinal)

Background

Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TFF3
Entrez
Uniprot