MUC3 Antibody (M3.1)

Images

 
Immunohistochemistry-Paraffin: MUC3 Antibody (M3.1) [NBP2-44432] - Human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (M3.1).
Immunohistochemistry-Paraffin: MUC3 Antibody (M3.1) [NBP2-44432] - Human Colon Carcinoma stained with MUC3 Monoclonal Antibody (M3.1).
Immunohistochemistry-Paraffin: MUC3 Antibody (M3.1) [NBP2-44432] - Human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (M3.1).
SDS-Page: MUC3 Antibody (M3.1) [NBP2-44432]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-P, PAGE, ICC/IF
Clone
M3.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

MUC3 Antibody (M3.1) Summary

Immunogen
A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
Localization
Cytoplasmic and cell surface
Specificity
It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC3A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • SDS-Page
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 7q22.1
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
1100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC3 Antibody (M3.1)

  • MUC-3A
  • mucin 3, intestinal
  • mucin 3A, cell surface associated
  • mucin 3A, intestinal
  • mucin-3A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47686AF350
Alexa Fluor 405 NBP2-47686AF405
Alexa Fluor 488 NBP2-47686AF488
Alexa Fluor 532 NBP2-47686AF532
Alexa Fluor 594 NBP2-47686AF594
Alexa Fluor 647 NBP2-47686AF647
Alexa Fluor 700 NBP2-47686AF700
Alexa Fluor 750 NBP2-47686AF750
Allophycocyanin NBP2-47686APC
Allophycocyanin/Cy7 NBP2-47686APCCY7
Biotin NBP2-47686B
DyLight 350 NBP2-47686UV
DyLight 405 NBP2-47686V
DyLight 488 NBP2-47686G
DyLight 550 NBP2-47686R
DyLight 594 NBP2-47686DL594
DyLight 650 NBP2-47686C
DyLight 680 NBP2-47686FR
DyLight 755 NBP2-47686IR
FITC NBP2-47686F
HRP NBP2-47686H
Janelia Fluor 549 NBP2-47686JF549
Janelia Fluor 646 NBP2-47686JF646
PE NBP2-47686PE
PE/Atto594 NBP2-47686PEATT594
PE/Cy5.5 NBP2-47686PECY55
PE/Cy7 NBP2-47686PECY7
PerCP NBP2-47686PCP

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Bioinformatics

Gene Symbol MUC3A
Uniprot