Immunocytochemistry/ Immunofluorescence: MUC2 Antibody [NBP1-31231] - MUC2 and Cadherin-17 were detected in immersion fixed, paraffin-embedded sections of MimEX adult stem cell-derived ascending colon tissue with Mouse ...read more
Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] - Paraffin-embedded human intestine. MUC2 antibody [C3], C-term diluted at 1:750.
Immunocytochemistry/ Immunofluorescence: MUC2 Antibody [NBP1-31231] - HeLa cells.Green: MUC2 protein stained by MUC2 antibody [C3], C-term. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] - Paraffin-embedded mouse intestine. MUC2 stained by MUC2 antibody [C3], C-term diluted at 1:2000
Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] - Paraffin-embedded mouse colon. MUC2 stained by MUC2 antibody [C3], C-term diluted at 1:2000.
IHC-Fr Assay dependent. Use in dot blot reported in scientific literature (PMID:32223302).
540 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-31231 in the following applications:
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for MUC2 Antibody
mucin 2, intestinal/tracheal
mucin 2, oligomeric mucus/gel-forming
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for MUC2 Antibody (NBP1-31231). (Showing 1 - 2 of 2 FAQs).
I want to know western blot of MUC2 (NBP1-31231) expression in which cell. I need a positive control.
A good positive control to use is Human Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. Please see UniProt Q02817.
I am doing western bot, but I don not know which line is MUC2, so I need positive control. Now I want to know how did you get the western blot image after adding anti-MUC2 (NBP1-31231)?
The best positive controls to use are Human Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. Unfortunately we do not have a lysate that could be used as a positive control. Our lab used the following Western Blot protocol as a general guide to get the results from the WB image.
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