MUC2 Antibody (JA10-92)

Images

 
Western Blot: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of MUC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary ...read more
Immunocytochemistry/ Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-MUC2 antibody. The section was pre-treated using heat ...read more
Flow Cytometry: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of Hela cells with MUC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa ...read more
Immunocytochemistry/ Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% ...read more
Immunocytochemistry/ Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human breast cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human colon cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human tonsil tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat brain tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat intestine tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunofluorescence staining of paraffin- embedded human colon using anti-MUC2 rabbit polyclonal antibody. The section was pre-treated using heat ...read more
Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MUC2 antibody. The section was pre-treated using heat mediated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JA10-92
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MUC2 Antibody (JA10-92) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within C-terminal Human MUC2. (SwissProt: Q02817 Human ;SwissProt: Q62635 Rat)
Localization
Secreted.
Specificity
MUC2 Antibody (JA10-91) is tissue specific for colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MUC2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
Theoretical MW
540 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-66961.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for MUC2 Antibody (JA10-92)

  • Intestinal mucin-2
  • MLP
  • MUC2
  • MUC-2
  • mucin 2, intestinal/tracheal
  • mucin 2, oligomeric mucus/gel-forming
  • mucin-2
  • SMUC

Background

MUC2, also called mucin 2, is a member of the mucin protein family which are heavily glycosylated, high molecular weight proteins that are produced by goblet cells in epithelial tissues including the intestinal and respiratory tract (1). Mucins can form gels and function in lubrication of mucosal surfaces which serve as a protective barrier of the epithelial lining and an innate host defense (1). Mucins are divided into two groups based on whether they are membrane-bound or secreted, where MUC2 belongs to the secretory group (1,2). Secreted mucins have a distinctive composition characterized by a large central exon "mucin domain" containing O-glycosylated tandem repeat regions rich in proline, threonine, and serine residues, non-repetitive domains, and cysteine-rich domains (1,2). The mucin domain is flanked by 5' and 3' D domains, a B-like domain, a C-like domain, and a Ck-domain (1,2). MUC2 was the first secretory mucin identified in humans (3,4). Full-length MUC2 protein is synthesized as 5179 amino acids (aa) in length with a theoretical molecular weight of ~540 kDa (5).

Changes or perturbations to MUC2 expression has been associated with a number of disease pathologies (1-4, 6). Specifically, altered MUC2 composition has been indicated in colorectal cancer, inflammatory bowel diseases (IBD) including ulcerative colitis and Chron's disease, and chronic obstructive pulmonary disease (COPD) (1-4, 6). In general, decreased or reduced MUC2 expression is associated with colorectal cancer disease progression and development of IBD (1-4, 6). Additionally, studies have found that upon intestinal infection due to parasites or bacteria MUC2 expression is increased, as is overall mucus secretion (3, 6).

References

1. Pothuraju, R., Krishn, S. R., Gautam, S. K., Pai, P., Ganguly, K., Chaudhary, S., Rachagani, S., Kaur, S., & Batra, S. K. (2020). Mechanistic and Functional Shades of Mucins and Associated Glycans in Colon Cancer. Cancers. https://doi.org/10.3390/cancers12030649

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8091447

3. Liu, Y., Yu, X., Zhao, J., Zhang, H., Zhai, Q., & Chen, W. (2020). The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.07.191

4. Allen, A., Hutton, D. A., & Pearson, J. P. (1998). The MUC2 gene product: a human intestinal mucin. The International Journal of Biochemistry & Cell Biology. https://doi.org/10.1016/s1357-2725(98)00028-4

5. Uniprot (Q02817)

6. Kim, Y. S., & Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports. https://doi.org/10.1007/s11894-010-0131-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MUC2 Antibody (NBP2-66961)(1)

We have publications tested in 1 application: ICC/IF.


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Bioinformatics

Gene Symbol MUC2