Human Colon Whole Tissue Lysate (Adult Whole Normal)

Western Blot: Human Colon Whole Tissue Lysate (Adult Normal) [NB820-59205] - analysis of Beta Actin in human colon tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.

SDS-Page: Human Colon Whole Tissue Lysate (Whole Normal) [NB820-59205]

• Reactivity: Hu
• Applications: WB
• Concentration: 5.0 mg/ml

Human Colon Whole Tissue Lysate (Adult Whole Normal) Summary

• Specificity: Total Protein - Human Adult Normal Tissue: Colon

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Western Blot Electrophoresis

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Digestive/Waste
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Colon Lysate (NB820-59205)(1)

Publication using NB820-59205

• Puvvula PK, Yu Y, Sullivan KR et al. Inhibiting an RBM39/MLL1 epigenomic regulatory complex with dominant-negative peptides disrupts cancer cell transcription and proliferation Cell reports 2021-06-01 [PMID: 34077726]

Review for Colon Lysate (NB820-59205) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/23/2014

Summary

• Application: Western Blot
• Sample Tested: Human Colon Whole Tissue Lysate (Adult Normal)
• Species: Human
• Lot: B607123