Human Colon Whole Tissue Lysate (Adult Whole Normal) Summary
Total Protein - Human Adult Normal Tissue: Colon
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.
Lysate Details for Colon
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
for 6 months from date of receipt.
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Colon Lysate (NB820-59205) (1)
Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.
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Reviews using NB820-59205:
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Ming Lu WB
Application Western Blot Sample Tested Human Colon Whole Tissue Lysate (Adult Normal) Species Human Lot B607123 Comments This Human Colon Whole Tissue Lysate (Adult Normal) is easy to work with. For 1mg, it will last for a long time if you load 20ug each time. Blocking
Blocking Details 2% cold fish geletin in TBS buffer, room temperature for 1hour Primary Anitbody
Dilution Ratio b-actin: Novusbio cat#NBP1-47423 1:1000, in blocking buffer 4C overnight Secondary Antibody
Secondary Description anti mouse light chain kappa Novus NB7549 Secondary Manufacturer Cat# Novus NB7549 Secondary Concentration 1:5000 Details
Detection Notes 1 min exposure gives band at correct MW. Comments
Comments This Human Colon Whole Tissue Lysate (Adult Normal) is easy to work with. For 1mg, it will last for a long time if you load 20ug each time.
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