Human Colon Whole Tissue Lysate (Adult Whole Normal)

Images

 
Western Blot: Human Colon Whole Tissue Lysate (Adult Normal) [NB820-59205] - analysis of Beta Actin in human colon tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa ...read more
SDS-Page: Human Colon Whole Tissue Lysate (Whole Normal) [NB820-59205]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Colon Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Colon

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Reviewed Applications
Read 1 Review rated 5
using
NB820-59205 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.
Concentration
5.0 mg/ml

Lysate Details for Colon

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Colon Lysate (NB820-59205) (0)

There are no publications for Colon Lysate (NB820-59205).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for Colon Lysate (NB820-59205) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB820-59205:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Colon NB820-59205
Enlarge
reviewed by:
Ming Lu
WB Human 10/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman Colon Whole Tissue Lysate (Adult Normal)
SpeciesHuman
LotB607123
CommentsThis Human Colon Whole Tissue Lysate (Adult Normal) is easy to work with. For 1mg, it will last for a long time if you load 20ug each time.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratiob-actin: Novusbio cat#NBP1-47423 1:1000, in blocking buffer 4C overnight

Secondary Antibody

Secondary Descriptionanti mouse light chain kappa Novus NB7549
Secondary Manufacturer Cat#Novus NB7549
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives band at correct MW.

Comments

CommentsThis Human Colon Whole Tissue Lysate (Adult Normal) is easy to work with. For 1mg, it will last for a long time if you load 20ug each time.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Colon Lysate (NB820-59205) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Colon Products

Blogs on Colon

There are no specific blogs for Colon, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ming Lu
10/23/2014
Application: WB
Species: Human