Human TFF3 Quantikine ELISA Kit



Product Details

Reactivity HuSpecies Glossary
Applications ELISA

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Human TFF3 Quantikine ELISA Kit Summary

The Quantikine Human TFF3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TFF3 in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human TFF3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFF3 showed linear curves that were parallel to the standard cur...ves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFF3.
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Natural and recombinant human Trefoil Factor 3 (TFF3)
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DTFF30.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TFF3 Quantikine ELISA Kit

  • HITF, human intestinal trefoil factor10Intestinal trefoil factor
  • ITF
  • TFF3
  • TFI
  • trefoil factor 3 (intestinal)


TFF3 (Trefoil Factor 3), also known as ITF and P1.B, is a peptide that plays an important role in epithelial regeneration and wound healing. It can form disulfide-linked dimers or associate into disulfide-linked complexes with the intestinal mucous proteins FCGBP and MUC-2. TFF3 is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach as well as by chondrocytes during bone development. TFF3 is upregulated in response to gastrointestinal epithelial disruptions and promotes epithelial wound healing. It enhances tumor cell invasion and metastasis by supporting hypoxia-induced VEGF upregulation.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for TFF3 (DTFF30)(6)

We have publications tested in 2 confirmed species: Human, Xenograft.

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Showing Publications 1 - 6 of 6.
Publications using DTFF30 Applications Species
F Cheng, X Wang, YS Chiou, C He, H Guo, YQ Tan, B Basappa, T Zhu, V Pandey, PE Lobie Trefoil factor 3 promotes pancreatic carcinoma progression via WNT pathway activation mediated by enhanced WNT ligand expression Cell Death & Disease, 2022;13(3):265. 2022 [PMID: 35332126] (Human) Human
RM Chen, YS Chiou, QY Chong, HM Poh, TZ Tan, MY Zhang, L Ma, T Zhu, V Pandey, Basappa, AP Kumar, PE Lobie Pharmacological Inhibition of TFF3 Enhances Sensitivity of CMS4 Colorectal Carcinoma to 5-Fluorouracil through Inhibition of p44/42 MAPK Int J Mol Sci, 2019;20(24):. 2019 [PMID: 31835445] (Xenograft) Xenograft
K Krüger, S Schmid, F Paulsen, A Ignatius, P Klinger, T Hotfiel, B Swoboda, K Gelse Trefoil Factor 3 (TFF3) Is Involved in Cell Migration for Skeletal Repair Int J Mol Sci, 2019;20(17):. 2019 [PMID: 31480518] (Human) Human
R Nakov, T Velikova, V Nakov, G Ianiro, V Gerova, L Tankova Serum trefoil factor 3 predicts disease activity in patients with ulcerative colitis Eur Rev Med Pharmacol Sci, 2019;23(2):788-794. 2019 [PMID: 30720187] (Human) Human
T Shimura, H Iwasaki, M Kitagawa, M Ebi, T Yamada, T Yamada, T Katano, H Nisie, Y Okamoto, K Ozeki, T Mizoshita, H Kataoka Urinary Cysteine-Rich Protein 61 and Trefoil Factor 3 as Diagnostic Biomarkers for Colorectal Cancer Transl Oncol, 2019;12(3):539-544. 2019 [PMID: 30611902] (Human) Human
Lebherz-Eichinger D, Tudor B, Ankersmit H, Reiter T, Haas M, Roth-Walter F, Krenn C, Roth G Trefoil Factor 1 Excretion Is Increased in Early Stages of Chronic Kidney Disease. PLoS ONE, 2015;10(9):e0138312. 2015 [PMID: 26390128]

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Gene Symbol TFF3