Human TFF3 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human TFF3 Quantikine ELISA Kit Summary

• Background: The Quantikine Human TFF3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TFF3 in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human TFF3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFF3 showed linear curves that were parallel to the standard cur...ves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFF3.
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• Specificity: Natural and recombinant human Trefoil Factor 3 (TFF3)
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TFF3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TFF3 Quantikine ELISA Kit

• HITF, human intestinal trefoil factor10Intestinal trefoil factor
• ITF
• TFF3
• TFI
• trefoil factor 3 (intestinal)

Background

TFF3 (Trefoil Factor 3), also known as ITF and P1.B, is a peptide that plays an important role in epithelial regeneration and wound healing. It can form disulfide-linked dimers or associate into disulfide-linked complexes with the intestinal mucous proteins FCGBP and MUC-2. TFF3 is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach as well as by chondrocytes during bone development. TFF3 is upregulated in response to gastrointestinal epithelial disruptions and promotes epithelial wound healing. It enhances tumor cell invasion and metastasis by supporting hypoxia-induced VEGF upregulation.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TFF3 (DTFF30)(9)

Publications using DTFF30

• Cristiani, CM;Calomino, C;Scaramuzzino, L;Murfuni, MS;Parrotta, EI;Bianco, MG;Cuda, G;Quattrone, A;Quattrone, A; Proximity Elongation Assay and ELISA for the Identification of Serum Diagnostic Biomarkers in Parkinson's Disease and Progressive Supranuclear Palsy International journal of molecular sciences 2024-10-30 [PMID: 39519214] (Human)
• Rogulska, K;Wojciechowska-Koszko, I;Krasnod?bska-Szponder, B;Kwiatkowski, P;Roszkowska, P;Do??gowska, B;?uczkowska, K;Machali?ski, B;Kosik-Bogacka, D; TFF3 as a Diagnostic Biomarker in Kidney Transplant Patients International journal of molecular sciences 2023-07-25 [PMID: 37569301] (Human)
• MM Hasan, MA Gazi, S Das, SM Fahim, F Hossaini, AR Khan, J Ferdous, MA Alam, M Mahfuz, T Ahmed Gut biomolecules (I-FABP, TFF3 and lipocalin-2) are associated with linear growth and biomarkers of environmental enteric dysfunction (EED) in Bangladeshi children Scientific Reports, 2022-08-16;12(1):13905. 2022-08-16 [PMID: 35974137] (Human)
• F Cheng, X Wang, YS Chiou, C He, H Guo, YQ Tan, B Basappa, T Zhu, V Pandey, PE Lobie Trefoil factor 3 promotes pancreatic carcinoma progression via WNT pathway activation mediated by enhanced WNT ligand expression Cell Death & Disease, 2022-03-25;13(3):265. 2022-03-25 [PMID: 35332126] (Human)
• RM Chen, YS Chiou, QY Chong, HM Poh, TZ Tan, MY Zhang, L Ma, T Zhu, V Pandey, Basappa, AP Kumar, PE Lobie Pharmacological Inhibition of TFF3 Enhances Sensitivity of CMS4 Colorectal Carcinoma to 5-Fluorouracil through Inhibition of p44/42 MAPK Int J Mol Sci, 2019-12-09;20(24):. 2019-12-09 [PMID: 31835445] (Xenograft)
• K Krüger, S Schmid, F Paulsen, A Ignatius, P Klinger, T Hotfiel, B Swoboda, K Gelse Trefoil Factor 3 (TFF3) Is Involved in Cell Migration for Skeletal Repair Int J Mol Sci, 2019-09-01;20(17):. 2019-09-01 [PMID: 31480518] (Human)
• R Nakov, T Velikova, V Nakov, G Ianiro, V Gerova, L Tankova Serum trefoil factor 3 predicts disease activity in patients with ulcerative colitis Eur Rev Med Pharmacol Sci, 2019-01-01;23(2):788-794. 2019-01-01 [PMID: 30720187] (Human)
• T Shimura, H Iwasaki, M Kitagawa, M Ebi, T Yamada, T Yamada, T Katano, H Nisie, Y Okamoto, K Ozeki, T Mizoshita, H Kataoka Urinary Cysteine-Rich Protein 61 and Trefoil Factor 3 as Diagnostic Biomarkers for Colorectal Cancer Transl Oncol, 2019-01-03;12(3):539-544. 2019-01-03 [PMID: 30611902] (Human)
• Lebherz-Eichinger D, Tudor B, Ankersmit H, Reiter T, Haas M, Roth-Walter F, Krenn C, Roth G Trefoil Factor 1 Excretion Is Increased in Early Stages of Chronic Kidney Disease. PLoS ONE, 2015-09-21;10(9):e0138312. 2015-09-21 [PMID: 26390128] (Human)

FAQs for TFF3 (DTFF30). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TFF3