MUC1 Antibody - BSA Free

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Knockout Validated: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 Antibody [NBP1-60046] - C57BL/6 Muc1 KO mice and WT mice were undergoing intranasal instillation of 75 ug of lipopolysaccharide (LPS) at day 1. Between ...read more
Genetic Strategies: Knockout Validated: MUC1 Antibody [NBP1-60046] - Dexamethasone induces Muc1-CT nuclear translocation and co-localization with GRa in bronchial epithelium of WT mice but not in MUC1 KO mice. ...read more
Western Blot: MUC-1 Antibody [NBP1-60046] - Total protein from mouse 3T3 cells and human HeLa cells, stomach and colon was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunocytochemistry/ Immunofluorescence: MUC1 Antibody [NBP1-60046] - Tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Cells were counterstained for alpha-tubulin against DyLight 550 (Red) and ...read more
Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Human kidney lysate tissue at an antibody concentration of 4-8ug/ml.
Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Pig stomach.
Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Pig stomach.
Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Human stomach.
MUC1 binds RIPK1 to mediate the RIPK1/RIPK3 pathway. (c,d) Normal 16HBE cells were treated with TNF-alpha for 24 hr, and the interaction between MUC1-CT and RIPK1/RIPK3 were confirmed by immunoprecipitation. Data are ...read more
PARKIN down regulation and impairment of mitophagy in DS fibroblasts.a, b Whole-cell extracts from 2 N and DS cells were analyzed by western blot for PARKIN and PINK1. Each quantitative data was normalized with ACTIN. ...read more
HIF-1 alpha -mediated repression of ENT1 expression in Caki-1 cells under hypoxia. Comparison of HIF-1 alpha and HIF-2 alpha protein expression in human matched renal normal-tumor samples (n=11) (A) and 3 RCC cell ...read more
Experimental setup and the effect of PHD3 silencing on 786-O proteome. a siRNA-mediated silencing of PHD3 protein level in 786-O ccRCC cell line using two individual siRNA sequences in normoxic and in hypoxic (1% O2) ...read more
Photomicrographs of immunostaining for osteocalcin and osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) and the investigated treatment (rows). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, Gp, Gt, Rb, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

MUC1 Antibody - BSA Free Summary

Immunogen
Synthetic peptides corresponding to a C terminal portion of the human MUC1 (between amino acids 1200-1250) [UniProt P15941].
Specificity
The immunogen for this antibody has 100% homology to human MUC1 isoforms S2, E2, 9, F, T10, 5, Y, Y-LSP, 8, 4, 3, 2, 1 and J13.
Predicted Species
Primate (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MUC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (PMID 28740504)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 4-8 ug/ml
  • Knockout Validated
  • Western Blot 0.2-1 ug/ml
Theoretical MW
122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-60046 in the following applications:

Reactivity Notes

Goat reactivity reported in scientific literature (PMID: 28740504). Predicted to react with bovine (82%) and lar gibbon (94%) based on immunogen sequence similarity.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MUC1 Antibody - BSA Free

  • Breast carcinoma-associated antigen DF3
  • Carcinoma-associated mucin
  • CD227 antigen
  • CD227
  • DF3 antigen
  • EMA
  • Episialin
  • H23 antigen
  • H23AG
  • KL-6
  • MAM6
  • MUC-1
  • MUC1/ZD
  • mucin 1, cell surface associated
  • mucin 1, transmembrane
  • Mucin-1
  • Peanut-reactive urinary mucin
  • PEM
  • PEMMUC-1/SEC
  • PEMT
  • Polymorphic epithelial mucin
  • PUMMUC-1/X
  • tumor associated epithelial mucin
  • Tumor-associated epithelial membrane antigen
  • Tumor-associated mucin

Background

Mucin 1 (MUC1), also known as episialin, EMA (epithelial membrane antigen), PEM (polymorphic epithelial mucin), and CA-15-3 antigen, is a membrane-bound type I transmembrane glycoprotein (1,2). MUC1 is typically expressed in the luminal or glandular epithelial cells of the gastrointestinal tract, breast, lungs, and more, and is often overexpressed in epithelial cancers, but also serves a protective role against infection and helps regulate inflammatory response (2,3). Human MUC1 is 1255 amino acids (aa) in length with a theoretical molecular weight of 122 kDa; however, depending on the amount of glycosylation can weigh between 250 - 500 kDa (2,4). Structurally, MUC1 consists of a N-terminal domain which contains a signal peptide, a variable number tandem repeat region (VNTR), and a SEA domain, as well a C-terminal domain which has the extracellular domain (ECD), transmembrane domain (TMD), and cytoplasmic tail (CT) (2,3). The VNTR is comprised of between 25 - 125 repeats of a 20 aa conserved sequence (3). MUC1 is heavily O-glycosylated in the VNTR and has moderate N-glycosylation sites following the VNTR and in the ECD (2). Glycosylation contributes to MUC1's functional properties (2). The MUC1 gene contains seven exons, giving rise to several MUC1 isoforms as a result of alternative splicing (2).

Overexpression of mucins, including MUC1, is a feature of many epithelial cancers (1,3,5,6). The presence of truncated glycan structures called tumor-associated carbohydrate antigens (TACAs) on MUC1 play a role in cancer progression and a loss of apical-basal polarity (5). Carbohydrate-binding partners called lectins are the primary binding partners of TACAs that give rise to the pro-tumor microenvironment and metastasis (5). Given this unique feature, TACAs are a potential target for cancer immunotherapies (5). There are a number of vaccines, drugs, and antibodies targeting MUC1 for treatment of a variety of cancers including breast, lung, and prostate (6). In addition to a role in cancer progression, MUC1, and specifically the CT portion, has been shown to have a positive, anti-inflammatory role in a variety of lung and airway infections (7).

References

1. Khodabakhsh, F., Merikhian, P., Eisavand, M. R., & Farahmand, L. (2021). Crosstalk between MUC1 and VEGF in angiogenesis and metastasis: a review highlighting roles of the MUC1 with an emphasis on metastatic and angiogenic signaling. Cancer cell international. https://doi.org/10.1186/s12935-021-01899-8

2. Nath, S., & Mukherjee, P. (2014). MUC1: a multifaceted oncoprotein with a key role in cancer progression. Trends in molecular medicine. https://doi.org/10.1016/j.molmed.2014.02.007

3. Dhar, P., & McAuley, J. (2019). The Role of the Cell Surface Mucin MUC1 as a Barrier to Infection and Regulator of Inflammation. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2019.00117

4. Uniprot (P15941)

5. Beckwith, D. M., & Cudic, M. (2020). Tumor-associated O-glycans of MUC1: Carriers of the glyco-code and targets for cancer vaccine design. Seminars in immunology. https://doi.org/10.1016/j.smim.2020.101389

6. Almasmoum H. (2021). The Roles of Transmembrane Mucins Located on Chromosome 7q22.1 in Colorectal Cancer. Cancer management and research. https://doi.org/10.2147/CMAR.S299089

7. Ballester, B., Milara, J., & Cortijo, J. (2021). The role of mucin 1 in respiratory diseases. European respiratory review : an official journal of the European Respiratory Society. https://doi.org/10.1183/16000617.0149-2020

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUC1 Antibody (NBP1-60046)(8)

We have publications tested in 4 confirmed species: Human, Mouse, Goat, Rabbit.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-Fr, WB.


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FLOW
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
WB
(3)
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Human
(3)
Mouse
(1)
Goat
(1)
Rabbit
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Showing Publications 1 - 8 of 8.
Publications using NBP1-60046 Applications Species
Guild J, Juul NH, Andalon A et al. Evidence for lung barrier regeneration by differentiation prior to binucleated and stem cell division The Journal of cell biology 2023-12-04 [PMID: 37843535]
Juul NH, Yoon JK, Martinez MC et al. KRAS(G12D) drives lepidic adenocarcinoma through stem-cell reprogramming Nature 2023-07-01 [PMID: 37468622] (IHC, Mouse) IHC Mouse
Sengupta A, Roldan N, Kiener M et al. A New Immortalized Human Alveolar Epithelial Cell Model to Study Lung Injury and Toxicity on a Breathing Lung-On-Chip System Frontiers in Toxicology 2022-06-17 [PMID: 35832493] (ICC/IF) ICC/IF
Sun YC, Hung KF, Li TY Et al. Transmembrane Mucin 1 Blocks Fluorescein Ingress to Corneal Epithelium Invest Ophthalmol Vis Sci 2022-02-25 [PMID: 35212722] (IHC-Fr, Rabbit)

Details:
Citation using the Biotin version of this antibody.
IHC-Fr Rabbit
Ballester B, Milara J, Cortijo J Pirfenidone anti-fibrotic effects are partially mediated by the inhibition of MUC1 bioactivation Oncotarget 2020-04-14 [PMID: 32341751] (WB, Human) WB Human
Zhang H, Ji J, Liu Q, Xu S. MUC1 downregulation promotes TNF-a-induced necroptosis in human bronchial epithelial cells via regulation of the RIPK1/RIPK3 pathway. J. Cell. Physiol. 2019-01-21 [PMID: 30666647] (WB, Human) WB Human
Milara J, Diaz-Platas L, Contreras S et al. MUC1 deficiency mediates corticosteroid resistance in chronic obstructive pulmonary disease. Respir. Res. 2018-11-20 [PMID: 30458870] (WB, Human) WB Human
Rosa F, Osorio JS, Trevisi E et al. 2, 4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis. PPAR Res. [PMID: 28740504] (FLOW, Goat) FLOW Goat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC1 Antibody (NBP1-60046). (Showing 1 - 1 of 1 FAQs).

  1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
    • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

Secondary Antibodies

 

Isotype Controls

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Research Areas for MUC1 Antibody (NBP1-60046)

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Blogs on MUC1.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

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Bioinformatics

Gene Symbol MUC1
Entrez
Uniprot