Mouse/Rat PDGF-BB Quantikine ELISA Kit

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat PDGF-BB Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse/Rat PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse/rat PDGF-BB in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat PDGF-BB and antibodies raised against recombinant human PDGF-BB. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural ...mouse/rat PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat PDGF-BB.
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• Specificity: Natural and recombinant mouse and rat PDGF-BB. This assay cross-reacts 10.2% with recombinant Rat PDGF-AB, 5.3% with recombinant Human PDGF-AB, 64.7% with recombinant Human PDGF-BB, 38.1% with natural Human PDGF and 65.2% with natural Porcine PDGF.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Pdgfb

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat PDGF-BB Quantikine ELISA Kit

• c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV
• PDGFBB
• PDGF-BB

Background

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for PDGF-BB (MBB00)(22)

Publications using MBB00

• R Abu Helal, HT Muturi, AD Lee, W Li, HE Ghadieh, SM Najjar Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Ceacam1 Gene International Journal of Molecular Sciences, 2022-04-14;23(8):. 2022-04-14 [PMID: 35457157] (Mouse)
• Y Feng, X Yang, J Huang, M Shen, L Wang, X Chen, Y Yuan, C Dong, X Ma, F Yuan Pharmacological Inhibition of Glutaminase 1 Attenuates Alkali-Induced Corneal Neovascularization by Modulating Macrophages Oxidative Medicine and Cellular Longevity, 2022-03-19;2022(0):1106313. 2022-03-19 [PMID: 35345831] (Mouse)
• RJHA Tillie, TL Theelen, K van Kuijk, L Temmerman, J de Bruijn, M Gijbels, C Betsholtz, EAL Biessen, JC Sluimer A Switch from Cell-Associated to Soluble PDGF-B Protects against Atherosclerosis, despite Driving Extramedullary Hematopoiesis Cells, 2021-07-10;10(7):. 2021-07-10 [PMID: 34359916] (Mouse)
• S Isozaki, H Konishi, M Fujiya, H Tanaka, Y Murakami, S Kashima, K Ando, N Ueno, K Moriichi, T Okumura Probiotic-Derived Polyphosphate Accelerates Intestinal Epithelia Wound Healing through Inducing Platelet-Derived Mediators Mediators of Inflammation, 2021-03-29;2021(0):5582943. 2021-03-29 [PMID: 33859537] (Rat)
• Y Yu, K Dai, Z Gao, W Tang, T Shen, Y Yuan, J Wang, C Liu Sulfated polysaccharide directs therapeutic angiogenesis via endogenous VEGF secretion of macrophages Science Advances, 2021-02-10;7(7):. 2021-02-10 [PMID: 33568481] (Mouse)
• W El Bakly, M Medhat, M Shafei, R Tash, M Elrefai, Y Shoukry, NN Omar Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis Heliyon, 2020-09-21;6(9):e05006. 2020-09-21 [PMID: 33005806] (Rat)
• K Hosaka, Y Yang, T Seki, Q Du, X Jing, X He, J Wu, Y Zhang, H Morikawa, M Nakamura, M Scherzer, X Sun, Y Xu, T Cheng, X Li, X Liu, Q Li, Y Liu, A Hong, Y Chen, Y Cao Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors Nat Commun, 2020-07-24;11(1):3704. 2020-07-24 [PMID: 32709869] (Mouse)
• KK Poh, PSS Lee, AH Djohan, MJ Galupo, GG Songco, TC Yeo, HC Tan, AM Richards, L Ye Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4 Cells, 2020-04-12;9(4):. 2020-04-12 [PMID: 32290541] (Rat)
• TL Kuo, KH Cheng, YS Shan, LT Chen, WC Hung ?-catenin-activated autocrine PDGF/Src signaling is a therapeutic target in pancreatic cancer Theranostics, 2019-01-01;9(2):324-336. 2019-01-01 [PMID: 30809277] (Mouse)
• X Zhou, RA Franklin, M Adler, JB Jacox, W Bailis, JA Shyer, RA Flavell, A Mayo, U Alon, R Medzhitov Circuit Design Features of a Stable Two-Cell System Cell, 2018-02-01;172(4):744-757.e17. 2018-02-01 [PMID: 29398113] (Mouse)
• NM El-Lakkany, WH El-Maadawy, SH Seif El-Di, OA Hammam, SH Mohamed, SM Ezzat, MM Safar, S Saleh Rosmarinic acid attenuates hepatic fibrogenesis via suppression of hepatic stellate cell activation/proliferation and induction of apoptosis Asian Pac J Trop Med, 2017-05-17;10(5):444-453. 2017-05-17 [PMID: 28647181] (Rat)
• Morgan A Urello Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides Bioeng Transl Med, 2016-10-19;1(2):207-219. 2016-10-19 [PMID: 27981245] (Mouse)
• Freeze-Dried Platelet-Rich Plasma Accelerates Bone Union with Adequate Rigidity in Posterolateral Lumbar Fusion Surgery Model in Rats Sci Rep, 2016-11-11;6(0):36715. 2016-11-11 [PMID: 27833116] (Rat)
• Koji Ueno Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts Sci Rep, 2016-06-22;6(0):28538. 2016-06-22 [PMID: 27329845] (Mouse)
• The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages Nat Commun, 2016-05-06;7(0):11385. 2016-05-06 [PMID: 27150562] (Mouse)
• Re S, Giordano G, Yakoub Y, Devosse R, Uwambayinema F, Couillin I, Ryffel B, Marbaix E, Lison D, Huaux F Uncoupling between inflammatory and fibrotic responses to silica: evidence from MyD88 knockout mice. PLoS ONE, 2014-07-22;9(7):e99383. 2014-07-22 [PMID: 25050810] (Mouse)
• Glinskii , Olga V, Huxley , Virginia, Glinskii , Vladimir, Rubin , Leona J, Glinsky , Vladisla Pulsed estrogen therapy prevents post-OVX porcine dura mater microvascular network weakening via a PDGF-BB-dependent mechanism. PLoS ONE, 2013-12-09;8(12):e82900. 2013-12-09 [PMID: 24349391] (Mouse)
• Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease. J. Clin. Invest., 2011-10-17;121(11):4548-66. 2011-10-17 [PMID: 22005303] (Mouse)
• Fujii T, Onimaru M, Yonemitsu Y, Kuwano H, Sueishi K Statins restore ischemic limb blood flow in diabetic microangiopathy via eNOS/NO upregulation but not via PDGF-BB expression. Am. J. Physiol. Heart Circ. Physiol., 2008-04-25;294(6):H2785-91. 2008-04-25 [PMID: 18441206] (Mouse)
• He M, Kubo H, Ishizawa K, Hegab AE, Yamamoto Y, Yamamoto H, Yamaya M The role of the receptor for advanced glycation end-products in lung fibrosis. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-10-19;293(6):L1427-36. 2007-10-19 [PMID: 17951314] (Mouse)
• Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy. Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74. 2007-10-17 [PMID: 17942672] (Mouse)
• Koyanagi T, Noguchi K, Ootani A, Inagaki K, Robbins RC, Mochly-Rosen D Pharmacological inhibition of epsilon PKC suppresses chronic inflammation in murine cardiac transplantation model. J. Mol. Cell. Cardiol., 2007-06-21;43(4):517-22. 2007-06-21 [PMID: 17655859] (Mouse)

FAQs for PDGF-BB (MBB00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Pdgfb