Mouse/Rat HGF Quantikine ELISA Kit

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat HGF Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse/Rat HGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat HGF in cell culture supernates, tissue homogenates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse HGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained usi...ng natural HGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.
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• Specificity: Natural and recombinant HGF. Recombinant mouse HGF single chain precursor cross-reacts 100% in this assay.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Hgf

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat HGF Quantikine ELISA Kit

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for HGF (MHG00)(31)

Publications using MHG00

• Qing, Z;Duan, J;Luo, Q;He, J;Huang, H;Zeng, Z; Mechanism of SENP1-Mediated Regulation of Liver Sinusoidal Endothelial Cells to Promote Regeneration Via the HIF-1? Signaling Pathway Archives of medical research 2025-08-22 [PMID: 40848695] (Mouse)
• Endo Kumata, Y;Inagaki, A;Nakamura, Y;Imura, T;Saito, R;Katano, T;Suzuki, S;Tokodai, K;Kamei, T;Unno, M;Watanabe, K;Tabata, Y;Goto, M; A novel method of pancreatic islet transplantation at the liver surface using a gelatin hydrogel nonwoven fabric Cell transplantation 2025-04-23 [PMID: 40264358] (Rat)
• Saito, R;Inagaki, A;Nakamura, Y;Imura, T;Kanai, N;Mitsugashira, H;Endo, Y;Katano, T;Suzuki, S;Tokodai, K;Kamei, T;Unno, M;Watanabe, K;Tabata, Y;Goto, M; Ideal Duration of Pretreatment Using a Gelatin Hydrogel Nonwoven Fabric Prior to Subcutaneous Islet Transplantation Cell transplantation 2023-07-19 [PMID: 37466120] (Mouse)
• Okui, T;Hiasa, M;Hata, K;Roodman, GD;Nakanishi, M;Yoneda, T; The acid-sensing nociceptor TRPV1 controls breast cancer progression in bone via regulating HGF secretion from sensory neurons Research square 2023-07-05 [PMID: 37461623] (Mouse)
• Kojima, H;Kushige, H;Yagi, H;Nishijima, T;Moritoki, N;Nagoshi, N;Nakano, Y;Tanaka, M;Hori, S;Hasegawa, Y;Abe, Y;Kitago, M;Nakamura, M;Kitagawa, Y; Combinational Treatment Involving Decellularized Extracellular Matrix Hydrogels With Mesenchymal Stem Cells Increased the Efficacy of Cell Therapy in Pancreatitis Cell transplantation 2023-05-16 [PMID: 37191199] (Rat)
• Y Iso, S Usui, H Suzuki Mesenchymal Stem/Stromal Cells in Skeletal Muscle Are Pro-Angiogenic, and the Effect Is Potentiated by Erythropoietin Pharmaceutics, 2023-03-24;15(4):. 2023-03-24 [PMID: 37111534] (Rat)
• H Masuo, A Shimizu, H Motoyama, K Kubota, T Notake, T Yoshizawa, K Hosoda, K Yasukawa, A Kobayashi, Y Soejima Impact of endothelial nitric oxide synthase activation on accelerated liver regeneration in a rat ALPPS model World Journal of Gastroenterology, 2023-02-07;29(5):867-878. 2023-02-07 [PMID: 36816620] (Rat)
• M Soltani, M Pourfarzam, AH Sharifabad, AK Neisiani, MK Mousavi, M Aliomrani Effect of pretreatment with a synbiotic on Perfluorooctanoic acid-induced liver damage after sub-acute oral exposure in C57BL/6J mice Toxicology and Applied Pharmacology, 2022-12-23;459(0):116360. 2022-12-23 [PMID: 36572227] (Mouse)
• T Suzuka, T Kotani, T Saito, S Matsuda, T Sato, T Takeuchi Therapeutic effects of adipose-derived mesenchymal stem/stromal cells with enhanced migration ability and hepatocyte growth factor secretion by low-molecular-weight heparin treatment in bleomycin-induced mouse models of systemic sclerosis Arthritis Research & Therapy, 2022-10-07;24(1):228. 2022-10-07 [PMID: 36207753] (Mouse)
• Y Matsuno, M Yanagihara, K Ueno, T Saito, H Kurazumi, R Suzuki, S Katsura, A Oga, K Hamano Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing Scientific Reports, 2022-07-22;12(1):12519. 2022-07-22 [PMID: 35869108] (Mouse)
• JH Jun, S Park, JY Kim, JY Lim, GT Park, JH Kim, GJ Kim Combination Therapy of Placenta-Derived Mesenchymal Stem Cells with WKYMVm Promotes Hepatic Function in a Rat Model with Hepatic Disease via Vascular Remodeling Cells, 2022-01-11;11(2):. 2022-01-11 [PMID: 35053347] (Rat)
• H Nagano, Y Suematsu, M Takuma, S Aoki, A Satoh, E Takayama, M Kinoshita, Y Morimoto, S Takeoka, T Fujie, T Kiyosawa Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds Scientific Reports, 2021-07-14;11(1):14500. 2021-07-14 [PMID: 34262089] (Mouse)
• R Zhang, J Jiang, Y Yu, F Wang, N Gao, Y Zhou, X Wan, Z Wang, P Wei, J Mei Analysis of structural components of decellularized scaffolds in renal fibrosis Bioactive materials, 2021-01-16;6(7):2187-2197. 2021-01-16 [PMID: 33511316] (Rat)
• T Saito, T Kotani, K Suzuki Antifibrotic therapy by sustained release of low molecular weight heparin from poly(lactic-co-glycolic acid) microparticles on bleomycin-induced pulmonary fibrosis in mice Sci Rep, 2020-11-04;10(1):19019. 2020-11-04 [PMID: 33149192] (Mouse)
• GW Park, J Heo, JY Kang, JW Yang, JS Kim, KD Kwon, BC Yu, SJ Lee Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns Sci Rep, 2020-07-24;10(1):12448. 2020-07-24 [PMID: 32709896] (Rat)
• H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto The neovascularization effect of dedifferentiated fat cells Sci Rep, 2020-06-08;10(1):9211. 2020-06-08 [PMID: 32514018] (Mouse)
• F Rohn, C Kordes, T Buschmann, D Reichert, M Wammers, G Poschmann, K Stühler, AS Benk, F Geiger, JP Spatz, D Häussinger Impaired integrin &alpha5 /&beta1 -mediated hepatocyte growth factor release by stellate cells of the aged liver Aging Cell, 2020-03-11;0(0):e13131. 2020-03-11 [PMID: 32157808] (Rat)
• BL Ecker, JY Lee, CJ Sterner, AC Solomon, DK Pant, F Shen, J Peraza, L Vaught, S Mahendra, GK Belka, TC Pan, KH Schmitz, LA Chodosh Impact of obesity on breast cancer recurrence and minimal residual disease Breast Cancer Res., 2019-03-13;21(1):41. 2019-03-13 [PMID: 30867005] (Mouse)
• J Tang, J Wang, K Huang, Y Ye, T Su, L Qiao, MT Hensley, TG Caranasos, J Zhang, Z Gu, K Cheng Cardiac cell-integrated microneedle patch for treating myocardial infarction Sci Adv, 2018-11-28;4(11):eaat9365. 2018-11-28 [PMID: 30498778] (Rat)
• X Chen, Y Zhang, W Wang, Z Liu, J Meng, Z Han Mesenchymal Stem Cells Modified with Heme Oxygenase-1 Have Enhanced Paracrine Function and Attenuate Lipopolysaccharide-Induced Inflammatory and Oxidative Damage in Pulmonary Microvascular Endothelial Cells Cell. Physiol. Biochem., 2018-08-28;49(1):101-122. 2018-08-28 [PMID: 30153667] (Rat)
• ME Alvarèz Fa, M Piccoli, C Franzin, A Sgrò, A Dedja, L Urbani, E Bertin, C Trevisan, P Gamba, AJ Burns, P De Coppi, M Pozzobon Decellularized Diaphragmatic Muscle Drives a Constructive Angiogenic Response In Vivo Int J Mol Sci, 2018-04-28;19(5):. 2018-04-28 [PMID: 29710813] (Mouse)
• M Ahmed, G Kumar, S Gourevitch, T Levchenko, E Galun, V Torchilin, SN Goldberg Radiofrequency ablation (RFA) induced systemic tumor growth can be reduced by suppression of resultant heat shock proteins Int J Hyperthermia, 2018-04-24;0(0):1-25. 2018-04-24 [PMID: 29631466] (Rat)
• YY Zhang, C Li, GF Yao, LJ Du, Y Liu, XJ Zheng, S Yan, JY Sun, Y Liu, MZ Liu, X Zhang, G Wei, W Tong, X Chen, Y Wu, S Sun, S Liu, Q Ding, Y Yu, H Yin, SZ Duan Deletion of Macrophage Mineralocorticoid Receptor Protects Hepatic Steatosis and Insulin Resistance through ER?/HGF/MET Pathway Diabetes, 2017-03-21;0(0):. 2017-03-21 [PMID: 28325853] (Mouse)
• Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation Proc. Natl. Acad. Sci. U.S.A, 2016-12-19;0(0):. 2016-12-19 [PMID: 27994152] (Rat)
• First steps to define murine amniotic fluid stem cell microenvironment Sci Rep, 2016-11-15;6(0):37080. 2016-11-15 [PMID: 27845396] (Mouse)
• BubR1 Insufficiency Impairs Liver Regeneration in Aged Mice after Hepatectomy through Intercalated Disc Abnormality Sci Rep, 2016-08-26;6(0):32399. 2016-08-26 [PMID: 27561386] (Mouse)
• Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux. PLoS ONE, 2015-05-04;10(5):e0125797. 2015-05-04 [PMID: 25938594] (Mouse)
• Cohen N, Zeng S, Seifert A, Kim T, Sorenson E, Greer J, Beckman M, Santamaria-Barria J, Crawley M, Green B, Rossi F, Besmer P, Antonescu C, DeMatteo R Pharmacological Inhibition of KIT Activates MET Signaling in Gastrointestinal Stromal Tumors. Cancer Res, 2015-04-02;75(10):2061-70. 2015-04-02 [PMID: 25836719] (Mouse)
• Ju , Guan-qun, Cheng , Jun, Zhong , Liang, Wu , Shuai, Zou , Xiang-yu, Zhang , Guang-yu, Gu , Di, Miao , Shuai, Zhu , Ying-jia, Sun , Jie, Du , Tao Microvesicles derived from human umbilical cord mesenchymal stem cells facilitate tubular epithelial cell dedifferentiation and growth via hepatocyte growth factor induction. PLoS ONE, 2015-03-20;10(3):e0121534. 2015-03-20 [PMID: 25793303] (Rat)
• The anti-fibrotic effects of mesenchymal stem cells on irradiated lungs via stimulating endogenous secretion of HGF and PGE2. Sci Rep, 2015-03-04;5(0):8713. 2015-03-04 [PMID: 25736907] (Rat)
• Garg R, Rennert R, Duscher D, Sorkin M, Kosaraju R, Auerbach L, Lennon J, Chung M, Paik K, Nimpf J, Rajadas J, Longaker M, Gurtner G Capillary force seeding of hydrogels for adipose-derived stem cell delivery in wounds. Stem Cells Transl Med, 2014-07-18;3(9):1079-89. 2014-07-18 [PMID: 25038246] (Mouse)

FAQs for HGF (MHG00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Hgf