Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit



Product Details

Reactivity Mu, RtSpecies Glossary
Applications ELISA

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Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit Summary

The Quantikine Mouse/Rat FGF basic Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat FGF basic in cell culture supernates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant rat FGF basic and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. ...Results obtained using natural FGF basic showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF basic.
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Natural and recombinant FGF basic.  This assay cross-reacts 100% with Bovine FGF basic, recombinant Human FGF basic is detectable.
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using MFB00.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • HBGF-2
  • heparin-binding growth factor 2
  • Prostatropin


FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for FGF basic/FGF2/bFGF (MFB00)(22)

We have publications tested in 2 confirmed species: Mouse, Rat.

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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using MFB00 Applications Species
Y Matsuno, M Yanagihara, K Ueno, T Saito, H Kurazumi, R Suzuki, S Katsura, A Oga, K Hamano Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing Scientific Reports, 2022;12(1):12519. 2022 [PMID: 35869108] (Mouse) Mouse
M Furuhata, T Takayama, T Yamamoto, Y Ozawa, M Senoo, M Ozaki, S Yamano, S Sato Real-time assessment of guided bone regeneration in critical size mandibular bone defects in rats using collagen membranes with adjunct fibroblast growth factor-2 Journal of dental sciences, 2021;16(4):1170-1181. 2021 [PMID: 34484585] (Rat) Rat
H Nagano, Y Suematsu, M Takuma, S Aoki, A Satoh, E Takayama, M Kinoshita, Y Morimoto, S Takeoka, T Fujie, T Kiyosawa Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds Scientific Reports, 2021;11(1):14500. 2021 [PMID: 34262089] (Mouse) Mouse
T Murakami, D Matsugami, W Yoshida, K Imamura, T Bizenjima, F Seshima, A Saito Healing of Experimental Periodontal Defects Following Treatment with Fibroblast Growth Factor-2 and Deproteinized Bovine Bone Mineral Biomolecules, 2021;11(6):. 2021 [PMID: 34072351] (Rat) Rat
HY Kim, SY Chun, EH Lee, B Kim, YS Ha, JW Chung, JN Lee, BS Kim, SH Oh, TG Kwon Bladder Regeneration Using a Polycaprolactone Scaffold with a Gradient Structure and Growth Factors in a Partially Cystectomized Rat Model J Korean Med Sci, 2020;35(41):e374. 2020 [PMID: 33107231] (Rat) Rat
R Zilkha-Fal, T Rachutin-Z, L Cleaver, M Gurevich, A Achiron RAM-589.555 favors neuroprotective and anti-inflammatory profile of CNS-resident glial cells in acute relapse EAE affected mice J Neuroinflammation, 2020;17(1):313. 2020 [PMID: 33081798] (Mouse) Mouse
YJ Park, HT An, JS Park, O Park, AJ Duh, K Kim, KH Chung, KC Lee, Y Oh, S Lee Tyrosine kinase inhibitor neratinib attenuates liver fibrosis by targeting activated hepatic stellate cells Sci Rep, 2020;10(1):14756. 2020 [PMID: 32901093] (Mouse) Mouse
N Firoozi, Y Kang Immobilization of FGF on Poly(xylitol dodecanedioic Acid) Polymer for Tissue Regeneration Sci Rep, 2020;10(1):10419. 2020 [PMID: 32591607] (Mouse) Mouse
Z Sun, Y Xie, RJ Lee, Y Chen, Q Jin, Q Lv, J Wang, Y Yang, Y Li, Y Cai, R Wang, Z Han, L Zhang, M Xie Myocardium-targeted transplantation of PHD2 shRNA-modified bone mesenchymal stem cells through ultrasound-targeted microbubble destruction protects the heart from acute myocardial infarction Theranostics, 2020;10(11):4967-4982. 2020 [PMID: 32308762] (Rat) Rat
A Shima, A Itou, S Takeuchi Cell fibers promote proliferation of co-cultured cells on a dish Sci Rep, 2020;10(1):288. 2020 [PMID: 31937888] (Mouse) Mouse
Show All 22 Publications.

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Product General Protocols

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FAQs for FGF basic/FGF2/bFGF (MFB00). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email to apply for your award, and we

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Gene Symbol Fgf2