The Quantikine Mouse/Rat CCL5/RANTES Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat RANTES levels in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant mouse RANTES as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant RANTES. Res...ults obtained using natural mouse or rat RANTES showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse and rat RANTES.
Natural and recombinant mouse and rat RANTES. This assay cross-reacts 1.3% with recombinant Human RANTES.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Mouse/Rat CCL5/RANTES Quantikine ELISA Kit, 2 Plate
chemokine (C-C motif) ligand 5
D17S136Enormally T-expressed, and presumably secreted
Eosinophil chemotactic cytokine
small inducible cytokine A5 (RANTES)
small inducible cytokine subfamily A (Cys-Cys), member 5
Small-inducible cytokine A5
T cell-specific protein P228
T-cell specific protein p288
TCP228T-cell-specific protein RANTES
RANTES (Regulated upon Activation, Normal T cell-expressed, and presumably Secreted), also
known as CCL5, a member of the CC chemokine family of inflammatory and immunoregulatory
cytokines, initially discovered by subtractive hybridization as a T cell-specific molecule (1, 2).
Mouse RANTES cDNA encodes a 91 amino acid (aa) residue precursor protein with a presumed
signal peptide of 23 aa residues that is cleaved to generate the 68 aa residue mature protein
(3). At the amino acid sequence level, mouse RANTES is 84% identical to human RANTES. Both
human and mouse RANTES exhibit cross species activity. Naturally occurring human RANTES
has been found to be a mixture of the 68 aa residue mature protein and a 66 aa residue
amino-terminally truncated form (4, 5). Cells known to express RANTES include keratinocytes,
eosinophils, platelets, endothelium, fibroblasts, respiratory epithelium, astrocytes, vascular
smooth muscle, and CD4+ and CD8+ T cells (6-14). As suggested by its acronym, RANTES
production by the various cell types is induced in response to cytokine stimulation (8-13).
Like other CC chemokines, RANTES is a monocyte chemoattractant (2). RANTES can also
chemoattract unstimulated CD4+/CD45RO+ memory T cells and stimulated CD4+ and CD8+
T cells with the naive and memory phenotypes. In addition, RANTES can chemoattract and
degranulate eosinophils, as well as chemoattract and induce histamine release from basophils.
Human RANTES has been shown to be an inhibitor of HIV infection of human mononuclear
cells (15, 16). Several CC chemokine receptors, including CCR-1, CCR-3, CCR-4, and CCR-5,
have been shown to bind RANTES and subsequently to transduce a signal by increasing the
intracellular calcium ion level (2, 17-21).
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