Mouse/Rat CCL5/RANTES Quantikine ELISA Kit, 2 Plate

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Mouse/Rat CCL5/RANTES Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse/Rat CCL5/RANTES Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat RANTES levels in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant mouse RANTES as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant RANTES. Res...ults obtained using natural mouse or rat RANTES showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse and rat RANTES.
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Specificity
Natural and recombinant mouse and rat RANTES.  This assay cross-reacts 1.3% with recombinant Human RANTES.
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Ccl5

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
MMR00 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat CCL5/RANTES Quantikine ELISA Kit, 2 Plate

  • CCL5
  • chemokine (C-C motif) ligand 5
  • D17S136Enormally T-expressed, and presumably secreted
  • EoCP
  • Eosinophil chemotactic cytokine
  • RANTES
  • SISd
  • SIS-delta
  • small inducible cytokine A5 (RANTES)
  • small inducible cytokine subfamily A (Cys-Cys), member 5
  • Small-inducible cytokine A5
  • T cell-specific protein P228
  • T-cell specific protein p288
  • TCP228T-cell-specific protein RANTES

Background

RANTES (Regulated upon Activation, Normal T cell-expressed, and presumably Secreted), also known as CCL5, a member of the CC chemokine family of inflammatory and immunoregulatory cytokines, initially discovered by subtractive hybridization as a T cell-specific molecule (1, 2). Mouse RANTES cDNA encodes a 91 amino acid (aa) residue precursor protein with a presumed signal peptide of 23 aa residues that is cleaved to generate the 68 aa residue mature protein (3). At the amino acid sequence level, mouse RANTES is 84% identical to human RANTES. Both human and mouse RANTES exhibit cross species activity. Naturally occurring human RANTES has been found to be a mixture of the 68 aa residue mature protein and a 66 aa residue amino-terminally truncated form (4, 5). Cells known to express RANTES include keratinocytes, eosinophils, platelets, endothelium, fibroblasts, respiratory epithelium, astrocytes, vascular smooth muscle, and CD4+ and CD8+ T cells (6-14). As suggested by its acronym, RANTES production by the various cell types is induced in response to cytokine stimulation (8-13). 
Like other CC chemokines, RANTES is a monocyte chemoattractant (2). RANTES can also chemoattract unstimulated CD4+/CD45RO+ memory T cells and stimulated CD4+ and CD8+ T cells with the naive and memory phenotypes. In addition, RANTES can chemoattract and degranulate eosinophils, as well as chemoattract and induce histamine release from basophils. Human RANTES has been shown to be an inhibitor of HIV infection of human mononuclear cells (15, 16). Several CC chemokine receptors, including CCR-1, CCR-3, CCR-4, and CCR-5, have been shown to bind RANTES and subsequently to transduce a signal by increasing the intracellular calcium ion level (2, 17-21).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL5/RANTES (MMR00)(80)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(2)
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Mouse
(73)
Rat
(6)
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Showing Publications 1 - 10 of 80. Show All 80 Publications.
Publications using MMR00 Applications Species
M Gasparitsc, A Schieber, T Schaubeck, U Keller, M Cattaruzza, B Lange-Sper Tyrphostin AG490 reduces inflammation and fibrosis in neonatal obstructive nephropathy PLoS ONE, 2019;14(12):e0226675. 2019 [PMID: 31846485] (Mouse) Mouse
GM Yu, W Tan Melatonin Inhibits Lipopolysaccharide-Induced Inflammation and Oxidative Stress in Cultured Mouse Mammary Tissue Mediators Inflamm., 2019;2019(0):8597159. 2019 [PMID: 30890898] (Mouse) Mouse
RL Openshaw, J Kwon, A McColl, JM Penninger, J Cavanagh, JA Pratt, BJ Morris JNK signalling mediates aspects of maternal immune activation: importance of maternal genotype in relation to schizophrenia risk J Neuroinflammation, 2019;16(1):18. 2019 [PMID: 30691477] (Mouse) Mouse
L Qin, Y Du, H Ding, A Haque, J Hicks, C Pedroza, C Mohan Bradykinin 1 receptor blockade subdues systemic autoimmunity, renal inflammation, and blood pressure in murine lupus nephritis Arthritis Res. Ther., 2019;21(1):12. 2019 [PMID: 30621761] (Mouse) Mouse
L Ritchie, R Tate, LH Chamberlai, G Robertson, M Zagnoni, T Sposito, S Wray, JA Wright, CE Bryant, NJ Gay, TJ Bushell Toll-like receptor 3 activation impairs excitability and synaptic activity via TRIF signalling in immature rat and human neurons Neuropharmacology, 2018;135(0):1-10. 2018 [PMID: 29505789] (Rat) Rat
IK Kim, K Kim, E Lee, DS Oh, CS Park, S Park, JM Yang, JH Kim, HS Kim, DT Shima, JH Kim, SH Hong, YH Cho, YH Kim, JB Park, GY Koh, YS Ju, HK Lee, S Lee, I Kim Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality J. Exp. Med., 2018;0(0):. 2018 [PMID: 29444818] (ELISA Capture, Mouse) ELISA Capture Mouse
F Allen, ID Bobanga, P Rauhe, D Barkauskas, N Teich, C Tong, J Myers, AY Huang CCL3 augments tumor rejection and enhances CD8+T cell infiltration through NK and CD103+dendritic cell recruitment via IFN? Oncoimmunology, 2018;7(3):e1393598. 2018 [PMID: 29399390] (ELISA Capture, Mouse) ELISA Capture Mouse
L Szulc-D?br, J Struzik, A Ostrowska, M Guzera, FN Toka, M Bossowska-, MM Giery?ska, A Winnicka, Z Nowak, MG Niemia?tow Functional paralysis of GM-CSF-derived bone marrow cells productively infected with ectromelia virus PLoS ONE, 2017;12(6):e0179166. 2017 [PMID: 28604814] (Mouse) Mouse
N Liu, L Xu, Y Shi, L Fang, H Gu, H Wang, X Ding, S Zhuang Pharmacologic targeting ERK1/2 attenuates the development and progression of hyperuricemic nephropathy in rats Oncotarget, 2017;8(20):33807-33826. 2017 [PMID: 28442634] (Rat) Rat
Y Pan, LJ Smithson, Y Ma, D Hambardzum, DH Gutmann Ccl5 establishes an autocrine high-grade glioma growth regulatory circuit critical for mesenchymal glioblastoma survival Oncotarget, 2017;0(0):. 2017 [PMID: 28380429] (Mouse) Mouse
Show All 80 Publications.

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Product General Protocols

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FAQs for CCL5/RANTES (MMR00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP MMR00

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Bioinformatics

Gene Symbol Ccl5