Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit Summary

• Background: The Quantikine^® Mouse Neutrophil Elastase/ELA2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure ELA2 in cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse ELA2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse ELA2. Results obtaine...d using natural mouse ELA2 showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ELA2.
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• Specificity: Natural and recombinant mouse ELA2.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit

• Bone marrow serine protease
• EC 3.4.21
• EC 3.4.21.37
• ELA2
• ELA2granulocyte-derived elastase
• ELANE
• elastase 2, neutrophil
• elastase, neutrophil expressed
• Elastase-2
• GE
• HLEelastase-2
• HNE
• Human leukocyte elastase
• Leukocyte Elastase
• Medullasin
• NE
• Neutrophil Elastase
• PMN elastase
• PMN-E
• polymorphonuclear elastase
• SCN1

Background

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Publications for Neutrophil Elastase/ELA2 (MELA20)(3)

Publications using MELA20

• Oishi, K;Yasui, H;Inoue, Y;Hozumi, H;Suzuki, Y;Karayama, M;Furuhashi, K;Enomoto, N;Fujisawa, T;Horinouchi, T;Iwaki, T;Suzuki, Y;Miyata, T;Inui, N;Suda, T; PAI-1 Inhibitor TM5441 Attenuates Emphysema and Airway Inflammation in a Murine Model of Chronic Obstructive Pulmonary Disease International journal of molecular sciences 2025-07-23 [PMID: 40806219] (Mouse)
• Pandey, N;Kaur, H;Chandaluri, L;Anand, SK;Chokhawala, H;Magdy, T;Stokes, KY;Orr, AW;Rom, O;Dhanesha, N; CD14 Blockade Does Not Improve Outcomes of Deep Vein Thrombosis Following Inferior Vena Cava Stenosis in Mice bioRxiv : the preprint server for biology 2024-01-12 [PMID: 38260582] (Mouse)
• T Lin, D Quellier, J Lamb, T Voisin, P Baral, F Bock, A Schönberg, R Mirchev, G Pier, I Chiu, M Gadjeva Pseudomonas aeruginosa-induced nociceptor activation increases susceptibility to infection PloS Pathogens, 2021-05-06;17(5):e1009557. 2021-05-06 [PMID: 33956874] (Mouse)

FAQs for Neutrophil Elastase/ELA2 (MELA20). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.