Mouse Lipocalin-2/NGAL Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse Lipocalin-2/NGAL Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse Lipocalin-2/NGAL Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Lipocalin-2 in cell culture supernates, mouse serum, platelet-poor plasma, and urine. It contains NS0-expressed recombinant mouse Lipocalin-2 and antibodies raised against the recombinant factor. Natural mouse Lipocalin-2 showed dose-response curves that were parallel to the standard c...urves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Lipocalin-2.
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• Specificity: Natural and recombinant mouse Lipocalin-2
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Lcn2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Lipocalin-2/NGAL Quantikine ELISA Kit

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Lipocalin-2/NGAL (MLCN20)(53)

Publications using MLCN20

• K Tange, S Yagi, E Takeshita, M Abe, Y Yamamoto, H Tomida, T Kawamura, M Hanayama, B Matsuura, Y Ikeda, Y Hiasa Oral administration of human carbonic anhydrase I suppresses colitis in a murine inflammatory bowel disease model Scientific Reports, 2022;12(1):17983. 2022 [PMID: 36289244] (Mouse)
• L Xu, J Guo, DG Moledina, LG Cantley Immune-mediated tubule atrophy promotes acute kidney injury to chronic kidney disease transition Nature Communications, 2022;13(1):4892. 2022 [PMID: 35986026] (Mouse)
• MY Chang, SH Hsu, LY Ma, LF Chou, CC Hung, YC Tian, CW Yang Effects of Suramin on Polycystic Kidney Disease in a Mouse Model of Polycystin-1 Deficiency International Journal of Molecular Sciences, 2022;23(15):. 2022 [PMID: 35955634] (Mouse)
• A Mazenc, L Mervant, C Maslo, C Lencina, V Bézirard, M Levêque, I Ahn, V Alquier-Ba, N Naud, C Héliès-Tou, L Debrauwer, S Chevolleau, F Guéraud, FHF Pierre, V Théodorou, M Olier Maternal heme-enriched diet promotes a gut pro-oxidative status associated with microbiota alteration, gut leakiness and glucose intolerance in mice offspring Redox Biology, 2022;53(0):102333. 2022 [PMID: 35588638] (Mouse)
• X Xiang, X Tang, Y Yu, S Xie, L Liu, M Chen, R Zhang, X Kang, Y Zheng, G Yang, S Gan, S Zhu Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microglia Journal of Neuroinflammation, 2022;19(1):92. 2022 [PMID: 35413913] (Mouse)
• TH Shen, J Stauber, K Xu, A Jacunski, N Paragas, M Callahan, R Banlengchi, AD Levitman, B Desanti de, A Beenken, MS Grau, E Mathieu, Q Zhang, Y Li, T Gopal, N Askanase, S Arumugam, S Mohan, PI Good, JS Stevens, F Lin, SK Sia, CS Lin, V D'Agati, K Kiryluk, NP Tatonetti, J Barasch Snapshots of nascent RNA reveal cell- and stimulus- specific responses to acute kidney injury JCI Insight, 2022;0(0):. 2022 [PMID: 35230973] (Mouse)
• TH Chen, CT Liu, CY Cheng, YM Sue, NJ Huang, CH Chen Oligosaccharides Ameliorate Acute Kidney Injury by Alleviating Cluster of Differentiation 44-Mediated Immune Responses in Renal Tubular Cells Nutrients, 2022;14(4):. 2022 [PMID: 35215410] (Rat)
• M Zingariell, P Verachi, F Gobbo, F Martelli, M Falchi, M Mazzarini, M Valeri, G Sarli, C Marinaccio, J Melo-Carde, JD Crispino, AR Migliaccio Resident Self-Tissue of Proinflammatory Cytokines Rather Than Their Systemic Levels Correlates with Development of Myelofibrosis in Gata1low Mice Biomolecules, 2022;12(2):. 2022 [PMID: 35204735] (Mouse)
• Y Zhan, M Zhu, S Liu, J Lu, Z Ni, H Cai, W Zhang MicroRNA?93 inhibits the apoptosis and inflammatory response of tubular epithelial cells via the PTEN/AKT/mTOR pathway in acute kidney injury Molecular Medicine Reports, 2021;24(3):. 2021 [PMID: 34296286] (Mouse)
• H Wang, N Banerjee, Y Liang, G Wang, KL Hoffman, MF Khan Gut microbiome-host interactions in driving environmental pollutant trichloroethene-mediated autoimmunity Toxicology and Applied Pharmacology, 2021;424(0):115597. 2021 [PMID: 34051218] (Mouse)
• N Fatima, S Patel, T Hussain Angiotensin AT2 Receptor is Anti-inflammatory and Reno-Protective in Lipopolysaccharide Mice Model: Role of IL-10 Frontiers in Pharmacology, 2021;12(0):600163. 2021 [PMID: 33935698] (Mouse)
• A Nakamura, S Kurihara, D Takahashi, W Ohashi, Y Nakamura, S Kimura, M Onuki, A Kume, Y Sasazawa, Y Furusawa, Y Obata, S Fukuda, S Saiki, M Matsumoto, K Hase Symbiotic polyamine metabolism regulates epithelial proliferation and macrophage differentiation in the colon Nature Communications, 2021;12(1):2105. 2021 [PMID: 33833232] (Mouse)
• L Gouweleeuw, H Wajant, O Maier, ULM Eisel, WM Blankestei, RG Schoemaker Effects of selective TNFR1 inhibition or TNFR2 stimulation, compared to non-selective TNF inhibition, on (neuro)inflammation and behavior after myocardial infarction in male mice Brain, Behavior, and Immunity, 2021;0(0):. 2021 [PMID: 33444731] (Mouse)
• JY Yoo, DR Cha, B Kim, EJ An, SR Lee, JJ Cha, YS Kang, JY Ghee, JY Han, YS Bae LPS-Induced Acute Kidney Injury Is Mediated by Nox4-SH3YL1 Cell Rep, 2020;33(3):108245. 2020 [PMID: 33086058] (Mouse)
• KL Otterpohl, BW Busselman, I Ratnayake, RG Hart, K Hart, C Evans, CL Phillips, JR Beach, P Ahrenkiel, B Molitoris, K Surendran, I Chandrasek Conditional Myh9 and Myh10 inactivation in adult mouse renal epithelium results in progressive kidney disease JCI Insight, 2020;0(0):. 2020 [PMID: 33001861] (Mouse)
• S Yeo, H Park, E Seo, J Kim, BK Kim, IS Choi, CS Huh Anti-Inflammatory and Gut Microbiota Modulatory Effect of Lactobacillus rhamnosus Strain LDTM 7511 in a Dextran Sulfate Sodium-Induced Colitis Murine Model Microorganisms, 2020;8(6):. 2020 [PMID: 32512895] (Mouse)
• SA Tanusepute, MT Lin, SL Yeh, CL Yeh Intravenous Arginine Administration Downregulates NLRP3 Inflammasome Activity and Attenuates Acute Kidney Injury in Mice with Polymicrobial Sepsis Mediators Inflamm., 2020;2020(0):3201635. 2020 [PMID: 32454788] (Mouse)
• T Cai, Q Ke, Y Fang, P Wen, H Chen, Q Yuan, J Luo, Y Zhang, Q Sun, Y Lv, K Zen, L Jiang, Y Zhou, J Yang Sodium-glucose cotransporter 2 inhibition suppresses HIF-1&alpha-mediated metabolic switch from lipid oxidation to glycolysis in kidney tubule cells of diabetic mice Cell Death Dis, 2020;11(5):390. 2020 [PMID: 32444604] (Human)
• D Lim, HK Kim, JH Jeong, YS Jung, SE Lee, HC Jang, SI Jung, HS Choi, JH Rhee, SG Lee, C Park, M Song, HE Choy Lipocalin2 Induced by Bacterial Flagellin Protects Mice against Cyclophosphamide Mediated Neutropenic Sepsis Microorganisms, 2020;8(5):. 2020 [PMID: 32365611] (Mouse)
• J Lv, ZL Wu, Z Gan, P Gui, SL Yao CXCL14 Overexpression Attenuates Sepsis-Associated Acute Kidney Injury by Inhibiting Proinflammatory Cytokine Production Mediators Inflamm., 2020;2020(0):2431705. 2020 [PMID: 32317861] (Mouse)
• M Ullah, DD Liu, S Rai, M Razavi, J Choi, J Wang, W Concepcion, AS Thakor A Novel Approach to Deliver Therapeutic Extracellular Vesicles Directly into the Mouse Kidney via Its Arterial Blood Supply Cells, 2020;9(4):. 2020 [PMID: 32290286] (Mouse)
• ME De Sousa R, MC Houser, DI Walker, DP Jones, J Chang, CJ Barnum, MG Tansey Targeting soluble tumor necrosis factor as a potential intervention to lower risk for late-onset Alzheimer's disease associated with obesity, metabolic syndrome, and type 2 diabetes Alzheimers Res Ther, 2019;12(1):1. 2019 [PMID: 31892368] (Mouse)
• L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking Cell Rep, 2019;29(7):2067-2077.e6. 2019 [PMID: 31722218] (Mouse)
• EC Lee, T Valencia, C Allerson, A Schairer, A Flaten, M Yheskel, K Kersjes, J Li, S Gatto, M Takhar, S Lockton, A Pavlicek, M Kim, T Chu, R Soriano, S Davis, JR Androsavic, S Sarwary, T Owen, J Kaplan, K Liu, G Jang, S Neben, P Bentley, T Wright, V Patel Discovery and preclinical evaluation of anti-miR-17 oligonucleotide RGLS4326 for the treatment of polycystic kidney disease Nat Commun, 2019;10(1):4148. 2019 [PMID: 31515477] (Mouse)
• T Ondee, J Gillen, P Visitchana, P Somparn, J Issara-Amp, C Dang Phi, W Chancharoe, D Gurusamy, A Nita-Lazar, A Leelahavan Lipocalin-2 (Lcn-2) Attenuates Polymicrobial Sepsis with LPS Preconditioning (LPS Tolerance) in FcGRIIb Deficient Lupus Mice Cells, 2019;8(9):. 2019 [PMID: 31514375] (Mouse)
• N Fuentes, N Cabello, M Nicoleau, ZC Chroneos, P Silveyra Modulation of the lung inflammatory response to ozone by the estrous cycle Physiol Rep, 2019;7(5):e14026. 2019 [PMID: 30848106] (Mouse)
• K Noh, H Lee, TY Choi, Y Joo, SJ Kim, H Kim, JY Kim, JW Jahng, S Lee, SY Choi, SJ Lee Negr1 controls adult hippocampal neurogenesis and affective behaviors Mol. Psychiatry, 2019;0(0):. 2019 [PMID: 30651602] (Mouse)
• N Makhezer, M Ben Khemis, D Liu, Y Khichane, V Marzaioli, A Tlili, M Mojallali, C Pintard, P Letteron, M Hurtado-Ne, J El-Benna, JC Marie, A Sannier, AL Pelletier, PM Dang NOX1-derived ROS drive the expression of Lipocalin-2 in colonic epithelial cells in inflammatory conditions Mucosal Immunol, 2018;0(0):. 2018 [PMID: 30279516] (Mouse)
• J Steenbrugg, K Breyne, K Demeyere, O De Wever, NN Sanders, W Van Den Br, C Colpaert, P Vermeulen, S Van Laere, E Meyer Anti-inflammatory signaling by mammary tumor cells mediates prometastatic macrophage polarization in an innovative intraductal mouse model for triple-negative breast cancer J. Exp. Clin. Cancer Res., 2018;37(1):191. 2018 [PMID: 30111338] (Mouse)
• BH Choi, Z Jin, CO Yi, J Oh, EA Jeong, JY Lee, KA Park, KE Kim, JE Lee, HJ Kim, JR Hahm, GS Roh Effects of lobeglitazone on insulin resistance and hepatic steatosis in high-fat diet-fed mice PLoS ONE, 2018;13(7):e0200336. 2018 [PMID: 29979770] (Mouse)
• A Sureshbabu, E Patino, KC Ma, K Laursen, EJ Finkelszte, O Akchurin, T Muthukumar, SW Ryter, L Gudas, AMK Choi, ME Choi RIPK3 promotes sepsis-induced acute kidney injury via mitochondrial dysfunction JCI Insight, 2018;3(11):. 2018 [PMID: 29875323] (Mouse)
• R Li, PS Kowalski, HWM Morselt, I Schepel, RM Jongman, A Aslan, MHJ Ruiters, JG Zijlstra, G Molema, M van Meurs, JAAM Kamps Endothelium-targeted delivery of dexamethasone by anti-VCAM-1 SAINT-O-Somes in mouse endotoxemia PLoS ONE, 2018;13(5):e0196976. 2018 [PMID: 29763440] (Mouse)
• JA Smith, A Braga, J Verheyen, S Basilico, S Bandiera, C Alfaro-Cer, L Peruzzotti, D Shu, F Haque, P Guo, S Pluchino RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity Mol Ther Nucleic Acids, 2018;10(0):103-121. 2018 [PMID: 29499926] (ELISA Capture, Mouse)
• H Meng, G Fu, J Shen, K Shen, Z Xu, Y Wang, B Jin, H Pan Ameliorative Effect of Daidzein on Cisplatin-Induced Nephrotoxicity in Mice via Modulation of Inflammation, Oxidative Stress, and Cell Death Oxid Med Cell Longev, 2017;2017(0):3140680. 2017 [PMID: 28831294] (Mouse)
• C Barthels, A Ogrinc, V Steyer, S Meier, F Simon, M Wimmer, A Blutke, T Straub, U Zimber-Str, E Lutgens, P Marconi, C Ohnmacht, D Garzetti, B Stecher, T Brocker CD40-signalling abrogates induction of ROR?t(+) Treg cells by intestinal CD103(+) DCs and causes fatal colitis Nat Commun, 2017;8(0):14715. 2017 [PMID: 28276457] (Mouse)
• S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment Cancer Res, 2017;0(0):. 2017 [PMID: 28249896] (Mouse)
• A Takahashi, Y Takabatake, T Kimura, I Maejima, T Namba, T Yamamoto, J Matsuda, S Minami, JY Kaimori, I Matsui, T Matsusaka, F Niimura, T Yoshimori, Y Isaka Autophagy Inhibits the Accumulation of Advanced Glycation End Products by Promoting Lysosomal Biogenesis and Function in the Kidney Proximal Tubules Diabetes, 2017;0(0):. 2017 [PMID: 28246295] (Mouse)
• N Maicas, J van der Vl, J Bublitz, S Florquin, M Bakker-van, CA Dinarello, V Verweij, R Masereeuw, LA Joosten, LB Hilbrands Human Alpha-1-Antitrypsin (hAAT) therapy reduces renal dysfunction and acute tubular necrosis in a murine model of bilateral kidney ischemia-reperfusion injury PLoS ONE, 2017;12(2):e0168981. 2017 [PMID: 28235038] (Mouse)
• Carlos A Roncal-Jim The effects of exogenous desmopressin on a model of heat stress nephropathy in mice Am. J. Physiol. Renal Physiol, 2016;0(0):ajprenal.0049. 2016 [PMID: 28003190] (Mouse)
• Obesity-induced kidney injury is attenuated by amelioration of aberrant PHD2 activation in proximal tubules Sci Rep, 2016;6(0):36533. 2016 [PMID: 27827416] (Mouse)
• Chronic Repression of mTOR Complex 2 Induces Changes in the Gut Microbiota of Diet-induced Obese Mice Sci Rep, 2016;6(0):30887. 2016 [PMID: 27471110] (Mouse)
• Tanu Parmar Acute Stress Responses Are Early Molecular Events of Retinal Degeneration in Abca4-/-Rdh8-/- Mice After Light Exposure Invest Ophthalmol Vis Sci, 2016;57(7):3257-67. 2016 [PMID: 27315541] (Mouse)
• Ishida T, Kotani H, Miyao M, Kawai C, Jemail L, Abiru H, Tamaki K Renal Impairment with Sublethal Tubular Cell Injury in a Chronic Liver Disease Mouse Model. PLoS ONE, 2016;11(1):e0146871. 2016 [PMID: 26752420] (Mouse)
• F Aigner Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation Am. J. Transplant., 2016;16(3):808-20. 2016 [PMID: 26595644] (Mouse)
• Nakamura I, Hama S, Itakura S, Takasaki I, Nishi T, Tabuchi Y, Kogure K Lipocalin2 as a plasma marker for tumors with hypoxic regions. Sci Rep, 2014;4(0):7235. 2014 [PMID: 25467539] (Mouse)
• Cheng L, Xing H, Mao X, Li L, Li X, Li Q Lipocalin-2 promotes m1 macrophages polarization in a mouse cardiac ischaemia-reperfusion injury model. Scand J Immunol, 2015;81(1):31-8. 2015 [PMID: 25359467] (Mouse)
• Miao Q, Ku A, Nishino Y, Howard J, Rao A, Shaver T, Garcia G, Le D, Karlin K, Westbrook T, Poli V, Nguyen H Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2. Nat Commun, 2014;5(0):4088. 2014 [PMID: 24909826] (Mouse)
• Fujimura K, Wakino S, Minakuchi H, Hasegawa K, Hosoya K, Komatsu M, Kaneko Y, Shinozuka K, Washida N, Kanda T, Tokuyama H, Hayashi K, Itoh H Ghrelin protects against renal damages induced by angiotensin-II via an antioxidative stress mechanism in mice. PLoS ONE, 2014;9(4):e94373. 2014 [PMID: 24747517] (Mouse)
• Urbschat A, Zacharowski K, Obermuller N, Rupprecht K, Penzkofer D, Jennewein C, Tran N, Scheller B, Dimmeler S, Paulus P The small fibrinopeptide Bbeta15-42 as renoprotective agent preserving the endothelial and vascular integrity in early ischemia reperfusion injury in the mouse kidney. PLoS ONE, 2014;9(1):e84432. 2014 [PMID: 24392138] (Mouse)
• Bhargava R, Altmann C, Andres-Hernando A, Webb R, Okamura K, Yang Y, Falk S, Schmidt E, Faubel S Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-alpha antibodies. PLoS ONE, 2013;8(11):e79037. 2013 [PMID: 24265742] (Mouse)
• Manohar M, Hirsh M, Chen Y, Woehrle T, Karande A, Junger W ATP release and autocrine signaling through P2X4 receptors regulate gammadelta T cell activation. J Leukoc Biol, 2012;92(4):787-94. 2012 [PMID: 22753954] (Mouse)
• Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus. Infect. Immun., 2012;80(1):410-7. 2012 [PMID: 22038916] (Mouse)
• Nairz M, Theurl I, Schroll A, Theurl M, Fritsche G, Lindner E, Seifert M, Crouch ML, Hantke K, Akira S, Fang FC, Weiss G Absence of functional Hfe protects mice from invasive Salmonella enterica serovar Typhimurium infection via induction of lipocalin-2. Blood, 2009;114(17):3642-51. 2009 [PMID: 19700664] (Mouse)

Bioinformatics

• Gene Symbol: Lcn2