Mouse LIF Quantikine ELISA Kit

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Mouse LIF Quantikine ELISA Kit Summary

Background
The Quantikine Mouse LIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIF levels in cell culture supernates, serum, and EDTA plasma. It contains E. coli-expressed recombinant mouse LIF and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse LIF showed linear curves that were parallel to the standard curves obtaine...d using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse LIF.
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Specificity
Natural and recombinant mouse LIF
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Lif

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MLF00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse LIF Quantikine ELISA Kit

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for LIF (MLF00)(8)

We have publications tested in 1 confirmed species: Mouse.


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Mouse
(8)
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Showing Publications 1 - 8 of 8.
Publications using MLF00 Applications Species
M Shoji, H Minato, S Ogaki, M Seki, Y Suzuki, S Kume, T Kuzuhara Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells PLoS ONE, 2018;13(7):e0201239. 2018 [PMID: 30048506] (Mouse) Mouse
Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial Infarction PLoS ONE, 2016;11(5):e0156562. 2016 [PMID: 27227407] (Mouse) Mouse
Yeo D, Kiparissides A, Cha J, Aguilar-Gallardo C, Polak J, Tsiridis E, Pistikopoulos E, Mantalaris A Improving embryonic stem cell expansion through the combination of perfusion and Bioprocess model design. PLoS ONE, 2013;8(12):e81728. 2013 [PMID: 24339957] (Mouse) Mouse
Quinton LJ, Mizgerd JP, Hilliard KL Leukemia inhibitory factor signaling is required for lung protection during pneumonia. J. Immunol., 2012;188(12):6300-8. 2012 [PMID: 22581855] (Mouse) Mouse
White CA, Zhang JG, Salamonsen LA, Baca M, Fairlie WD, Metcalf D, Nicola NA, Robb L, Dimitriadis E Blocking LIF action in the uterus by using a PEGylated antagonist prevents implantation: a nonhormonal contraceptive strategy. Proc. Natl. Acad. Sci. U.S.A., 2007;104(49):19357-62. 2007 [PMID: 18042698] (Mouse) Mouse
Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD Astrocytes promote myelination in response to electrical impulses. Neuron, 2006;49(6):823-32. 2006 [PMID: 16543131] (Mouse) Mouse
Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ Leukemia inhibitory factor is linked to regulatory transplantation tolerance. Transplantation, 2005;79(6):726-30. 2005 [PMID: 15785381] (Mouse) Mouse
Metcalfe SM, Muthukumarana PA, Thompson HL, Haendel MA, Lyons GE Leukaemia inhibitory factor (LIF) is functionally linked to axotrophin and both LIF and axotrophin are linked to regulatory immune tolerance. FEBS Lett., 2005;579(3):609-14. 2005 [PMID: 15670816] (Mouse) Mouse

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Product General Protocols

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FAQs for LIF (MLF00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP MLF00

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Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

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Bioinformatics

Gene Symbol Lif