Mouse LIF Quantikine ELISA Kit Summary
The Quantikine Mouse LIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIF levels
in cell culture supernates, serum, and EDTA plasma. It contains E. coli-expressed recombinant mouse LIF and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse LIF showed linear curves that were parallel to the standard curves obtaine ... d using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse LIF.
Natural and recombinant mouse LIF
Solid Phase Sandwich ELISA
No significant interference observed with available related molecules.
Packaging, Storage & Formulations
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Mouse LIF Quantikine ELISA Kit
CDF D Factor DIA differentiation inhibitory activity differentiation stimulating factor Differentiation-stimulating factor Emfilermin HILDA HILDAcholinergic differentiation factor leukemia inhibitory factor (cholinergic differentiation factor) leukemia inhibitory factor LIF Melanoma-derived LPL inhibitor MLPLI Background
Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Publications for LIF (MLF00)(8)
We have publications tested in 1 confirmed species: Mouse.
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Publications using MLF00
M Shoji, H Minato, S Ogaki, M Seki, Y Suzuki, S Kume, T Kuzuhara Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells PLoS ONE, 2018;13(7):e0201239. 2018 [PMID: 30048506] (Mouse)
Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial Infarction PLoS ONE, 2016;11(5):e0156562. 2016 [PMID: 27227407] (Mouse)
Yeo D, Kiparissides A, Cha J, Aguilar-Gallardo C, Polak J, Tsiridis E, Pistikopoulos E, Mantalaris A Improving embryonic stem cell expansion through the combination of perfusion and Bioprocess model design. PLoS ONE, 2013;8(12):e81728. 2013 [PMID: 24339957] (Mouse)
Quinton LJ, Mizgerd JP, Hilliard KL Leukemia inhibitory factor signaling is required for lung protection during pneumonia. J. Immunol., 2012;188(12):6300-8. 2012 [PMID: 22581855] (Mouse)
White CA, Zhang JG, Salamonsen LA, Baca M, Fairlie WD, Metcalf D, Nicola NA, Robb L, Dimitriadis E Blocking LIF action in the uterus by using a PEGylated antagonist prevents implantation: a nonhormonal contraceptive strategy. Proc. Natl. Acad. Sci. U.S.A., 2007;104(49):19357-62. 2007 [PMID: 18042698] (Mouse)
Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD Astrocytes promote myelination in response to electrical impulses. Neuron, 2006;49(6):823-32. 2006 [PMID: 16543131] (Mouse)
Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ Leukemia inhibitory factor is linked to regulatory transplantation tolerance. Transplantation, 2005;79(6):726-30. 2005 [PMID: 15785381] (Mouse)
Metcalfe SM, Muthukumarana PA, Thompson HL, Haendel MA, Lyons GE Leukaemia inhibitory factor (LIF) is functionally linked to axotrophin and both LIF and axotrophin are linked to regulatory immune tolerance. FEBS Lett., 2005;579(3):609-14. 2005 [PMID: 15670816] (Mouse)
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Product General Protocols
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FAQs for LIF (MLF00). (Showing 1 -
1 of 1 FAQs).
wondering what the difference is between your quantikine and duo set elisas?
Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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