Mouse LIF Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse LIF Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse LIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIF levels in cell culture supernates, serum, and EDTA plasma. It contains E. coli-expressed recombinant mouse LIF and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse LIF showed linear curves that were parallel to the standard curves obtaine...d using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse LIF.
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• Specificity: Natural and recombinant mouse LIF
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Lif

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse LIF Quantikine ELISA Kit

• CDF
• D Factor
• DIA
• differentiation inhibitory activity
• differentiation stimulating factor
• Differentiation-stimulating factor
• Emfilermin
• HILDA
• HILDAcholinergic differentiation factor
• leukemia inhibitory factor (cholinergic differentiation factor)
• leukemia inhibitory factor
• LIF
• Melanoma-derived LPL inhibitor
• MLPLI

Background

Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for LIF (MLF00)(16)

Publications using MLF00

• Kogai, H;Tsukamoto, S;Koga, M;Miyano, M;Akagi, T;Yamaguchi, A;Mori, K;Gotoh, K;Nakazawa, Y; Broad-spectrum efficacy of CEACAM6-targeted antibody-drug conjugate with BET protein degrader in colorectal, lung, and breast cancer mouse models Molecular cancer therapeutics 2025-01-15 [PMID: 39812376] (Mouse, Xenograft)
• Kwon, YY;Hui, S; IL-6 is dispensable for causing cachexia in the colon carcinoma 26 model bioRxiv : the preprint server for biology 2023-05-02 [PMID: 37205425] (Mouse)
• S Xu, X Yang, Q Chen, Z Liu, Y Chen, X Yao, A Xiao, J Tian, L Xie, M Zhou, Z Hu, F Zhu, X Xu, F Hou, J Nie Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis EBioMedicine, 2022-11-04;86(0):104312. 2022-11-04 [PMID: 36335669] (Mouse)
• P Li, Q Li, N Biswas, H Xin, T Diemer, L Liu, L Perez Guti, G Paternostr, C Piermarocc, S Domanskyi, RK Wang, N Ferrara LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy Embo Molecular Medicine, 2021-11-15;0(0):e14511. 2021-11-15 [PMID: 34779136] (Bovine, Mouse)
• JW Lee, J Hur, YW Kwon, CW Chae, JI Choi, I Hwang, JY Yun, JA Kang, YE Choi, YH Kim, SE Lee, C Lee, DH Jo, H Seok, BS Cho, SH Baek, HS Kim KAI1(CD82) is a key molecule to control angiogenesis and switch angiogenic milieu to quiescent state Journal of hematology & oncology, 2021-09-16;14(1):148. 2021-09-16 [PMID: 34530889] (Mouse)
• J Wang, GR Sareddy, Y Lu, UP Pratap, F Tang, KM Greene, PL Meyre, RR Tekmal, RK Vadlamudi, DW Brann Astrocyte-Derived Estrogen Regulates Reactive Astrogliosis and is Neuroprotective Following Ischemic Brain Injury J Neurosci, 2020-11-06;0(0):. 2020-11-06 [PMID: 33158962] (Mouse)
• SS Welc, I Flores, M Wehling-He, J Ramos, Y Wang, C Bertoni, JG Tidball Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy Nat Commun, 2019-06-26;10(1):2788. 2019-06-26 [PMID: 31243277] (Mouse, Transgenic Mouse)
• M Shoji, H Minato, S Ogaki, M Seki, Y Suzuki, S Kume, T Kuzuhara Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells PLoS ONE, 2018-07-26;13(7):e0201239. 2018-07-26 [PMID: 30048506] (Mouse)
• Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial Infarction PLoS ONE, 2016-05-26;11(5):e0156562. 2016-05-26 [PMID: 27227407] (Mouse)
• Wu L, Ong S, Talor M, Barin J, Baldeviano G, Kass D, Bedja D, Zhang H, Sheikh A, Margolick J, Iwakura Y, Rose N, Cihakova D Cardiac fibroblasts mediate IL-17A-driven inflammatory dilated cardiomyopathy. J Exp Med, 2014-06-16;211(7):1449-64. 2014-06-16 [PMID: 24935258] (Mouse)
• Yeo D, Kiparissides A, Cha J, Aguilar-Gallardo C, Polak J, Tsiridis E, Pistikopoulos E, Mantalaris A Improving embryonic stem cell expansion through the combination of perfusion and Bioprocess model design. PLoS ONE, 2013-12-10;8(12):e81728. 2013-12-10 [PMID: 24339957] (Mouse)
• Quinton LJ, Mizgerd JP, Hilliard KL Leukemia inhibitory factor signaling is required for lung protection during pneumonia. J. Immunol., 2012-05-11;188(12):6300-8. 2012-05-11 [PMID: 22581855] (Mouse)
• White CA, Zhang JG, Salamonsen LA, Baca M, Fairlie WD, Metcalf D, Nicola NA, Robb L, Dimitriadis E Blocking LIF action in the uterus by using a PEGylated antagonist prevents implantation: a nonhormonal contraceptive strategy. Proc. Natl. Acad. Sci. U.S.A., 2007-11-27;104(49):19357-62. 2007-11-27 [PMID: 18042698] (Mouse)
• Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD Astrocytes promote myelination in response to electrical impulses. Neuron, 2006-03-16;49(6):823-32. 2006-03-16 [PMID: 16543131] (Mouse)
• Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ Leukemia inhibitory factor is linked to regulatory transplantation tolerance. Transplantation, 2005-03-27;79(6):726-30. 2005-03-27 [PMID: 15785381] (Mouse)
• Metcalfe SM, Muthukumarana PA, Thompson HL, Haendel MA, Lyons GE Leukaemia inhibitory factor (LIF) is functionally linked to axotrophin and both LIF and axotrophin are linked to regulatory immune tolerance. FEBS Lett., 2005-01-31;579(3):609-14. 2005-01-31 [PMID: 15670816] (Mouse)

FAQs for LIF (MLF00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Lif