Mouse Leptin DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Leptin DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Lep

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Leptin DuoSet ELISA, 15 Plate

• FLJ94114
• LEP
• leptin (murine obesity homolog)
• leptin (obesity homolog, mouse)
• Leptin
• OB
• Obese protein
• obese, mouse, homolog of
• Obesity factor
• OBOBS

Background

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

Publications for Leptin/OB (DY498)(40)

Publications using DY498

• Honce, R;Vazquez-Pagan, A;Livingston, B;Mandarano, AH;Wilander, BA;Cherry, S;Hargest, V;Sharp, B;Brigleb, PH;Kirkpatrick Roubidoux, E;Van de Velde, LA;Skinner, RC;McGargill, MA;Thomas, PG;Schultz-Cherry, S; Diet switch pre-vaccination improves immune response and metabolic status in formerly obese mice Nature microbiology 2024-04-18 [PMID: 38637722] (Mouse)
• Lee, YR;Lee, HB;Oh, MJ;Kim, Y;Park, HY; Thyme Extract Alleviates High-Fat Diet-Induced Obesity and Gut Dysfunction Nutrients 2023-12-04 [PMID: 38068865] (Mouse)
• Ribeiro, R;Silva, EG;Moreira, FC;Gomes, GF;Cussat, GR;Silva, BSR;da Silva, MCM;de Barros Fernandes, H;de Sena Oliveira, C;de Oliveira Guarnieri, L;Lopes, V;Ferreira, CN;de Faria, AMC;Maioli, TU;Ribeiro, FM;de Miranda, AS;Moraes, GSP;de Oliveira, ACP;Vieira, LB; Chronic hyperpalatable diet induces impairment of hippocampal-dependent memories and alters glutamatergic and fractalkine axis signaling Scientific reports 2023-09-29 [PMID: 37773430] (Mouse)
• Deng, L;Wu, SA;Qi, L;Kersten, S; HILPDA is a lipotoxic marker in adipocytes that mediates the autocrine negative feedback regulation of triglyceride hydrolysis by fatty acids and alleviates cellular lipotoxic stress Molecular metabolism 2023-07-06 [PMID: 37422000] (Mouse)
• Martinez-Lopez, N;Mattar, P;Toledo, M;Bains, H;Kalyani, M;Aoun, ML;Sharma, M;McIntire, LBJ;Gunther-Cummins, L;Macaluso, FP;Aguilan, JT;Sidoli, S;Bourdenx, M;Singh, R; mTORC2-NDRG1-CDC42 axis couples fasting to mitochondrial fission Nature cell biology 2023-06-29 [PMID: 37386153] (Mouse)
• TS Huang, T Wu, YD Wu, XH Li, J Tan, CH Shen, SJ Xiong, ZQ Feng, SF Gao, H Li, WB Cai Long-term statins administration exacerbates diabetic nephropathy via ectopic fat deposition in diabetic mice Nature Communications, 2023-01-24;14(1):390. 2023-01-24 [PMID: 36693830] (Mouse)
• A Marquez, E Andrada, M Russo, ML Bolondi, E Fabersani, R Medina, P Gauffin-Ca Characterization of autochthonous lactobacilli from goat dairy products with probiotic potential for metabolic diseases Heliyon, 2022-08-28;8(9):e10462. 2022-08-28 [PMID: 36091951] (Mouse)
• L Orliaguet, T Ejlalmanes, A Humbert, R Ballaire, M Diedisheim, JB Julla, D Chokr, J Cuenco, J Michielett, J Charbit, D Lindén, J Boucher, C Potier, A Hamimi, S Lemoine, C Blugeon, P Legoix, S Lameiras, LG Baudrin, S Baulande, A Soprani, FA Castelli, F Fenaille, JP Riveline, E Dalmas, J Rieusset, JF Gautier, N Venteclef, F Alzaid Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling Nature Communications, 2022-08-30;13(1):5089. 2022-08-30 [PMID: 36042203] (Mouse)
• MD Ihrie, VL McQuade, JT Womble, A Hegde, MS McCravy, CVG Lacuesta, RM Tighe, LG Que, JKL Walker, JL Ingram Exogenous leptin enhances markers of airway fibrosis in a mouse model of chronic allergic airways disease Respiratory Research, 2022-05-24;23(1):131. 2022-05-24 [PMID: 35610699] (Mouse)
• CM Percopo, M McCullough, AR Limkar, KM Druey, HF Rosenberg Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice PLoS ONE, 2021-08-12;16(8):e0255997. 2021-08-12 [PMID: 34383839] (Mouse)
• K Kuramoto, YJ Kim, JH Hong, C He The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding Cell Reports, 2021-05-25;35(8):109184. 2021-05-25 [PMID: 34038729] (Mouse)
• A Schmid, M Roderfeld, J Gehl, E Roeb, A Nist, HR Chung, T Stiewe, T Karrasch, A Schäffler C1q/TNF-Related Protein 3 (CTRP-3) Deficiency of Adipocytes Affects White Adipose Tissue Mass but Not Systemic CTRP-3 Concentrations International Journal of Molecular Sciences, 2021-02-07;22(4):. 2021-02-07 [PMID: 33562308] (Mouse)
• XAMH van Dieren, T Sancerni, MC Alves-Guer, R Stienstra The role of uncoupling protein 2 in macrophages and its impact on obesity-induced adipose tissue inflammation and insulin resistance J Biol Chem, 2020-01-01;0(0):. 2020-01-01 [PMID: 33051201] (Mouse)
• YC Cheng, JR Liu Effect of Lactobacillus rhamnosus GG on Energy Metabolism, Leptin Resistance, and Gut Microbiota in Mice with Diet-Induced Obesity Nutrients, 2020-08-24;12(9):. 2020-08-24 [PMID: 32846917] (Mouse)
• XAMH van Dieren, MA de la Rosa, A Georgiadi, F Mattijssen, W Dijk, M van Weeghe, R Singh, JW Borst, R Stienstra, S Kersten HILPDA Uncouples Lipid Droplet Accumulation in Adipose Tissue Macrophages from Inflammation and Metabolic Dysregulation Cell Rep, 2020-02-11;30(6):1811-1822.e6. 2020-02-11 [PMID: 32049012] (Mouse)
• S Ali, AG Mansour, W Huang, NJ Queen, X Mo, JM Anderson, QN Hassan Ii, RS Patel, RK Wilkins, MA Caligiuri, L Cao CSF1R inhibitor PLX5622 and environmental enrichment additively improve metabolic outcomes in middle-aged female mice Aging (Albany NY), 2020-02-02;12(0):. 2020-02-02 [PMID: 32007953] (Mouse)
• CH Val, MC de Oliveir, DR Lacerda, A Barroso, NV Batista, Z Menezes-Ga, DRR de Assis, AT Cramer, F Brant, MM Teixeira, D Glória Sou, AM Ferreira, FS Machado SOCS2 modulates adipose tissue inflammation and expansion in mice J. Nutr. Biochem., 2019-11-23;76(0):108304. 2019-11-23 [PMID: 31816561] (Mouse)
• W Huang, NJ Queen, TB McMurphy, S Ali, L Cao Adipose PTEN regulates adult adipose tissue homeostasis and redistribution via a PTEN-leptin-sympathetic loop Mol Metab, 2019-09-28;30(0):48-60. 2019-09-28 [PMID: 31767180] (Mouse)
• NJ Queen, AA Boardman, RS Patel, JJ Siu, X Mo, L Cao Environmental enrichment improves metabolic and behavioral health in the BTBR mouse model of autism Psychoneuroendocrinology, 2019-10-15;111(0):104476. 2019-10-15 [PMID: 31648110] (Mouse)
• TDSM Farias, RID Paixao, MM Cruz, RDCDC de Sa, JJ Simão, VJ Antraco, MIC Alonso-Val Melatonin Supplementation Attenuates the Pro-Inflammatory Adipokines Expression in Visceral Fat from Obese Mice Induced by A High-Fat Diet Cells, 2019-09-06;8(9):. 2019-09-06 [PMID: 31489938] (Mouse)
• T Keeley, A Kirov, WY Koh, V Demambro, I Bergquist, J Cotter, P Caradonna, ME Siviski, B Best, T Henderson, CJ Rosen, L Liaw, I Prudovsky, DJ Small Resistance to visceral obesity is associated with increased locomotion in mice expressing an endothelial cell-specific fibroblast growth factor 1 transgene Physiol Rep, 2019-04-01;7(7):e14034. 2019-04-01 [PMID: 30972920] (Mouse)
• K Drareni, R Ballaire, S Barilla, MJ Mathew, A Toubal, R Fan, N Liang, C Chollet, Z Huang, M Kondili, F Foufelle, A Soprani, R Roussel, JF Gautier, F Alzaid, E Treuter, N Venteclef GPS2 Deficiency Triggers Maladaptive White Adipose Tissue Expansion in Obesity via HIF1A Activation Cell Rep, 2018-09-11;24(11):2957-2971.e6. 2018-09-11 [PMID: 30208320] (Mouse)
• LA Biondo, HA Batatinha, CO Souza, AAS Teixeira, LS Silveira, MI Alonso-Val, LM Oyama, MJ Alves, M Seelaender, JCR Neto Metformin Mitigates Fibrosis and Glucose Intolerance Induced by Doxorubicin in Subcutaneous Adipose Tissue Front Pharmacol, 2018-05-08;9(0):452. 2018-05-08 [PMID: 29867463] (Mouse)
• SL Christian, NK Pallegar, RJ Brown, AM Viloria-Pe Collagen overlays can inhibit leptin and adiponectin secretion but not lipid accumulation in adipocytes PeerJ, 2018-04-27;6(0):e4641. 2018-04-27 [PMID: 29719734] (Mouse)
• L Boutens, GJ Hooiveld, S Dhingra, RA Cramer, MG Netea, R Stienstra Unique metabolic activation of adipose tissue macrophages in obesity promotes inflammatory responses Diabetologia, 2018-01-14;0(0):. 2018-01-14 [PMID: 29333574] (Mouse)
• A Imai, K Satoi, E Fujimoto, K Sato Inducing maternal inflammation promotes leptin production in offspring but does not improve allergic symptoms in a mouse model of allergic rhinitis Heliyon, 2017-06-22;3(6):e00327. 2017-06-22 [PMID: 28707000] (Mouse)
• W Huang, X Liu, NJ Queen, L Cao Targeting Visceral Fat by Intraperitoneal Delivery of Novel AAV Serotype Vector Restricting Off-Target Transduction in Liver Mol Ther Methods Clin Dev, 2017-06-19;6(0):68-78. 2017-06-19 [PMID: 28702474] (Mouse)
• LN Masi, AR Martins, AR Crisma, CL do Amaral, MR Davanso, TDA Serdan, RDC da Cunha d, MM Cruz, MIC Alonso-Val, RP Torres, J Mancini-Fi, JNB Pereira, MM da Silva R, EA Liberti, SM Hirabara, R Curi Combination of a high-fat diet with sweetened condensed milk exacerbates inflammation and insulin resistance induced by each separately in mice Sci Rep, 2017-06-21;7(1):3937. 2017-06-21 [PMID: 28638152] (Mouse)
• Travis B McMurphy Hepatic Expression of Adenovirus 36 E4ORF1 Improves Glycemic Control and Promotes Glucose Metabolism via AKT Activation Diabetes, 2016-11-30;0(0):. 2016-11-30 [PMID: 27903748] (Mouse)
• Fairbridge N, Southall T, Ayre D, Komatsu Y, Raquet P, Brown R, Randell E, Kovacs C, Christian S Loss of CD24 in Mice Leads to Metabolic Dysfunctions and a Reduction in White Adipocyte Tissue. PLoS ONE, 2015-11-04;10(11):e0141966. 2015-11-04 [PMID: 26536476] (Mouse)
• Kim J, Sohn J, Lee J, Park J Obesity increases airway hyperresponsiveness via the TNF-alpha pathway and treating obesity induces recovery. PLoS ONE, 2015-02-06;10(2):e0116540. 2015-02-06 [PMID: 25658739] (Mouse)
• Barra , Nicole G, Palanivel , Rengasam, Denou , Emmanuel, Chew , Marianne, Gillgrass , Amy, Walker , Tina D, Kong , Josh, Richards , Carl D, Jordana , Manel, Collins , Stephen, Trigatti , Bernardo, Holloway , Alison C, Raha , Sandeep, Steinberg , Gregory, Ashkar , Ali A Interleukin-15 modulates adipose tissue by altering mitochondrial mass and activity. PLoS ONE, 2014-12-17;9(12):e114799. 2014-12-17 [PMID: 25517731] (Mouse)
• Liu , Xianglan, McMurphy , Travis, Xiao , Run, Slater , Andrew, Huang , Wei, Cao , Lei Hypothalamic gene transfer of BDNF inhibits breast cancer progression and metastasis in middle age obese mice. Mol Ther, 2014-03-18;22(7):1275-84. 2014-03-18 [PMID: 24637454] (Mouse)
• Dorn C, Engelmann J, Saugspier M, Koch A, Hartmann A, Muller M, Spang R, Bosserhoff A, Hellerbrand C Increased expression of c-Jun in nonalcoholic fatty liver disease. Lab Invest, 2014-02-03;94(4):394-408. 2014-02-03 [PMID: 24492282] (Mouse)
• Menezes-Garcia Z, Oliveira M, Lima R, Soriani F, Cisalpino D, Botion L, Teixeira M, Souza D, Ferreira A Lack of platelet-activating factor receptor protects mice against diet-induced adipose inflammation and insulin-resistance despite fat pad expansion. Obesity (Silver Spring), 2013-12-14;22(3):663-72. 2013-12-14 [PMID: 24339378] (Mouse)
• Csoka B, Koscso B, Toro G, Kokai E, Virag L, Nemeth Z, Pacher P, Bai P, Hasko G A2B adenosine receptors prevent insulin resistance by inhibiting adipose tissue inflammation via maintaining alternative macrophage activation. Diabetes, 2013-11-05;63(3):850-66. 2013-11-05 [PMID: 24194503] (Mouse)
• Freeman , Linnea R, Zhang , Le, Dasuri , Kalavath, Fernandez-Kim , Sun-Ok, Bruce-Keller , Annadora, Keller , Jeffrey Mutant amyloid precursor protein differentially alters adipose biology under obesogenic and non-obesogenic conditions. PLoS ONE, 2012-08-17;7(8):e43193. 2012-08-17 [PMID: 22912823] (Mouse)
• Pini M, Rhodes DH, Castellanos KJ Rosiglitazone improves survival and hastens recovery from pancreatic inflammation in obese mice. PLoS ONE, 2012-07-16;7(7):e40944. 2012-07-16 [PMID: 22815875] (Mouse)
• Kuo LE, Kitlinska JB, Tilan JU, Li L, Baker SB, Johnson MD, Lee EW, Burnett MS, Fricke ST, Kvetnansky R, Herzog H, Zukowska Z Neuropeptide Y acts directly in the periphery on fat tissue and mediates stress-induced obesity and metabolic syndrome. Nat. Med., 2007-07-01;13(7):803-11. 2007-07-01 [PMID: 17603492] (Mouse)
• Lu FL, Johnston RA, Flynt L, Theman TA, Terry RD, Schwartzman IN, Lee A, Shore SA Increased pulmonary responses to acute ozone exposure in obese db/db mice. Am. J. Physiol. Lung Cell Mol. Physiol., 2005-12-22;290(5):L856-65. 2005-12-22 [PMID: 16373670] (Mouse)

FAQs for Leptin/OB (DY498). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: Lep