Mouse L-Selectin/CD62L DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse L-Selectin/CD62L DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Sell

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse L-Selectin/CD62L DuoSet ELISA, 15 Plate

• CD62L antigen
• CD62L
• gp90-MEL
• hLHRc
• LAM1
• LAM-1
• LAM1LECAM1
• LECAM1
• LEU8
• Leu-8
• Leukocyte adhesion molecule 1
• Leukocyte surface antigen Leu-8
• Leukocyte-endothelial cell adhesion molecule 1
• LNHR
• LNHRTQ1
• LSEL
• L-Selectin
• LYAM1
• Lyam-1
• LYAM1CD62 antigen-like family member L
• Lymph node homing receptor
• lymphocyte adhesion molecule 1
• pln homing receptor
• PLNHR
• selectin L
• SELL
• TQ1

Background

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

Publications for L-Selectin/CD62L (DY576)(4)

Publications using DY576

• CC Chao, R Yang, Z Zhang, T Belinskaya, CT Chan, SA Miller, R Hammamieh, M Jett, WM Ching Temporal analysis of mRNA expression profiles in Orientia infected C3HeB/FeJ mouse BMC Microbiol., 2020-01-06;20(1):3. 2020-01-06 [PMID: 31906849] (Mouse)
• Tumor-induced MDSC act via remote control to inhibit L-selectin-dependent adaptive immunity in lymph nodes Elife, 2016-12-08;5(0):. 2016-12-08 [PMID: 27929373] (Mouse)
• Arndt PG, Strahan B, Wang Y, Long C, Horiuchi K, Walcheck B Leukocyte ADAM17 Regulates Acute Pulmonary Inflammation. PLoS ONE, 2011-05-16;6(5):e19938. 2011-05-16 [PMID: 21603616] (Mouse)
• Sinclair LV, Finlay D, Feijoo C, Cornish GH, Gray A, Ager A, Okkenhaug K, Hagenbeek TJ, Spits H, Cantrell DA Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking. Nat. Immunol., 2008-04-06;9(5):513-21. 2008-04-06 [PMID: 18391955] (Mouse)

FAQs for L-Selectin/CD62L (DY576). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Sell