Mouse IL-10 Quantikine ELISA Kit, 2 Plate

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Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse IL-10 Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-10 accurately. Results obtained using natural mouse IL-1...0 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-10.
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Specificity
Natural and recombinant mouse IL-10
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il10

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
M1000B in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-10 Quantikine ELISA Kit, 2 Plate

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat IL-10 and 70-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta. IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL 10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-10 (M1000B)(194)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: ELISA Capture.


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Human
(1)
Mouse
(191)
Rat
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Showing Publications 1 - 10 of 194. Show All 194 Publications.
Publications using M1000B Applications Species
R Morales, C Duran-Anio, J Bravo-Aleg, LD Estrada, M Shahnawaz, PP Hu, C Kramm, D Morales-Sc, A Urayama, C Soto Infusion of blood from mice displaying cerebral amyloidosis accelerates amyloid pathology in animal models of Alzheimer's disease Acta neuropathologica communications, 2020;8(1):213. 2020 [PMID: 33287898] (Mouse) Mouse
N Hagan, JL Kane, D Grover, L Woodworth, C Madore, J Saleh, J Sancho, J Liu, Y Li, J Proto, M Zelic, A Mahan, M Kothe, AA Scholte, M Fitzgerald, B Gisevius, A Haghikia, O Butovsky, D Ofengeim CSF1R signaling is a regulator of pathogenesis in progressive MS Cell Death Dis, 2020;11(10):904. 2020 [PMID: 33097690] (Mouse) Mouse
F Petrillo, MC Trotta, C Bucolo, A Hermenean, A Petrillo, R Maisto, G Pieretti, M Pietropaol, F Ferraracci, C Gagliano, M Galdiero, M D'Amico Resolvin D1 attenuates the inflammatory process in mouse model of LPS-induced keratitis J Cell Mol Med, 2020;0(0):. 2020 [PMID: 33058526] (Mouse) Mouse
SS El-Hawary, M Sobeh, WK Badr, MAO Abdelfatta, ZY Ali, ME El-Tantawy, MA Rabeh, M Wink HPLC-PDA-MS/MS profiling of secondary metabolites from Opuntia ficus-indica cladode, peel and fruit pulp extracts and their antioxidant, neuroprotective effect in rats with aluminum chloride induced neurotoxicity Saudi J Biol Sci, 2020;27(10):2829-2838. 2020 [PMID: 32994743] (Rat) Rat
R Li, Y Li, X Dong Preconditioning mesenchymal stromal cells with flagellin enhances the anti?inflammatory ability of their secretome against lipopolysaccharide?induced acute lung injury Molecular Medicine Reports, 2020;0(0):. 2020 [PMID: 32945411] (Mouse) Mouse
SW Jin, GH Lee, MJ Jang, GE Hong, JY Kim, GD Park, H Jin, HS Kim, JH Choi, CY Choi, SG Lee, HG Jeong, YP Hwang Immunomodulatory Activity of Lactococcus lactis GCWB1176 in Cyclophosphamide-Induced Immunosuppression Model Microorganisms, 2020;8(8):. 2020 [PMID: 32748895] (Mouse) Mouse
J Xiao, G Zhang, S Gao, J Shen, H Feng, Z He, C Xu Combined administration of SHP2 inhibitor SHP099 and the &alpha7nAChR agonist PNU282987 protect mice against DSS?induced colitis Mol Med Rep, 2020;22(3):2235-2244. 2020 [PMID: 32705242] (Mouse) Mouse
P Clua, M Tomokiyo, F Raya Tonet, MA Islam, V García Cas, G Marcial, S Salva, S Alvarez, H Takahashi, S Kurata, H Kitazawa, J Villena The Role of Alveolar Macrophages in the Improved Protection against Respiratory Syncytial Virus and Pneumococcal Superinfection Induced by the Peptidoglycan of Lactobacillus rhamnosus CRL1505 Cells, 2020;9(7):. 2020 [PMID: 32660087] (Mouse) Mouse
M Nakao, T Miyagaki, M Sugaya, S Sato Exacerbated Imiquimod-Induced Psoriasis-Like Skin Inflammation in IRF5-Deficient Mice Int J Mol Sci, 2020;21(10):. 2020 [PMID: 32456211] (Mouse) Mouse
P Gurung, S Dahal, P Chaudhary, D Guragain, U Karmachary, JA Kim, BS Jeong Potent Inhibitory Effect of BJ-3105, a 6-Alkoxypyridin-3-ol Derivative, on Murine Colitis Is Mediated by Activating AMPK and Inhibiting NOX Int J Mol Sci, 2020;21(9):. 2020 [PMID: 32365634] (Mouse) Mouse
Show All 194 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-10 (M1000B). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP M1000B
HRP M1000B
HRP M1000B

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Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

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PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
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Bioinformatics

Gene Symbol Il10