Mouse CXCL9/MIG Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse CXCL9/MIG Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MIG in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIG and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural m...ouse MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MIG.
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• Specificity: Natural and recombinant mouse MIG
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Cxcl9

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CXCL9/MIG Quantikine ELISA Kit

• chemokine (C-X-C motif) ligand 9
• CMK
• crg-10
• C-X-C motif chemokine 9
• CXCL9
• Gamma-interferon-induced monokine
• Humig
• MIG
• MIGSmall-inducible cytokine B9
• monokine induced by gamma interferon
• SCYB9Monokine induced by interferon-gamma

Background

CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL9/MIG (MCX900)(28)

Publications using MCX900

• Krzyzowska, M;Janicka, M;Chodkowski, M;Patrycy, M;Obuch-Woszczaty?ska, O;Tomaszewska, E;Ranoszek-Soliwoda, K;Celichowski, G;Grobelny, J; Epigallocatechin Gallate-Modified Silver Nanoparticles Show Antiviral Activity against Herpes Simplex Type 1 and 2 Viruses 2023-09-29 [PMID: 37896801] (Mouse)
• Sha, L;Li, J;Shen, H;Wang, Q;Meng, P;Zhang, X;Deng, Y;Zhu, W;Xu, Q; LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis Cell reports 2023-08-11 [PMID: 37573508] (Mouse)
• N Ishikura, M Sugimoto, K Yorozu, M Kurasawa, O Kondoh Anti?VEGF antibody triggers the effect of anti?PD?L1 antibody in PD?L1low and immune desert?like mouse tumors Oncology reports, 2021-12-27;47(2):. 2021-12-27 [PMID: 34958105] (Mouse)
• M Pein, J Insua-Rodr, T Hongu, A Riedel, J Meier, L Wiedmann, K Decker, MAG Essers, HP Sinn, S Spaich, M Sütterlin, A Schneeweis, A Trumpp, T Oskarsson Metastasis-initiating cells induce and exploit a fibroblast niche to fuel malignant colonization of the lungs Nat Commun, 2020-03-20;11(1):1494. 2020-03-20 [PMID: 32198421] (Mouse)
• L Qin, Y Du, H Ding, A Haque, J Hicks, C Pedroza, C Mohan Bradykinin 1 receptor blockade subdues systemic autoimmunity, renal inflammation, and blood pressure in murine lupus nephritis Arthritis Res. Ther., 2019-01-08;21(1):12. 2019-01-08 [PMID: 30621761] (Mouse)
• K Kanai, AM Park, A Watanabe, T Arikawa, T Yasui, H Yoshida, I Tsunoda, O Yoshie Murine ?-Herpesvirus 68 Induces Severe Lung Inflammation in IL-27-Deficient Mice with Liver Dysfunction Preventable by Oral Neomycin J. Immunol., 2018-03-02;0(0):. 2018-03-02 [PMID: 29500240] (Mouse)
• C Alanio, R Barreira d, D Michonneau, P Bousso, MA Ingersoll, ML Albert CXCR3/CXCL10 Axis Shapes Tissue Distribution of Memory Phenotype CD8+ T Cells in Nonimmunized Mice J. Immunol., 2017-11-29;0(0):. 2017-11-29 [PMID: 29187588] (Mouse)
• D Tajfirouz, DM West, XT Yin, CA Potter, R Klein, PM Stuart CXCL9 compensates for the absence of CXCL10 during recurrent Herpetic stromal keratitis Virology, 2017-03-07;506(0):7-13. 2017-03-07 [PMID: 28282568] (Mouse)
• CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata J Immunol, 2016-07-13;0(0):. 2016-07-13 [PMID: 27412416] (Mouse)
• Krauthausen M, Kummer M, Zimmermann J, Reyes-Irisarri E, Terwel D, Bulic B, Heneka M, Muller M CXCR3 promotes plaque formation and behavioral deficits in an Alzheimer's disease model. J Clin Invest, 2014-12-15;125(1):365-78. 2014-12-15 [PMID: 25500888] (Mouse)
• Jayaraman A, Jackson D, Message S, Pearson R, Aniscenko J, Caramori G, Mallia P, Papi A, Shamji B, Edwards M, Westwick J, Hansel T, Stanciu L, Johnston S, Bartlett N IL-15 complexes induce NK- and T-cell responses independent of type I IFN signaling during rhinovirus infection. Mucosal Immunol, 2014-01-29;7(5):1151-64. 2014-01-29 [PMID: 24472849] (Mouse)
• Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol, 2014-01-17;192(4):1671-80. 2014-01-17 [PMID: 24442435] (Mouse)
• Guirnalda P, Wood L, Goenka R, Crespo J, Paterson Y Interferon gamma-induced intratumoral expression of CXCL9 alters the local distribution of T cells following immunotherapy with Oncoimmunology, 2013-07-23;2(8):e25752. 2013-07-23 [PMID: 24083082] (Mouse)
• Gottfries J, Melgar S, Michaelsson E Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease. PLoS ONE, 2012-01-18;7(1):e30005. 2012-01-18 [PMID: 22279558] (Mouse)
• Wallace KL, Marshall MA, Ramos SI, Lannigan JA, Field JJ, Strieter RM, Linden J NKT cells mediate pulmonary inflammation and dysfunction in murine sickle cell disease through production of IFN-gamma and CXCR3 chemokines. Blood, 2009-05-11;114(3):667-76. 2009-05-11 [PMID: 19433855] (Mouse)
• Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis. Am. J. Pathol., 2008-04-10;172(5):1209-21. 2008-04-10 [PMID: 18403600] (Mouse)
• Campanella GS, Tager AM, El Khoury JK, Thomas SY, Abrazinski TA, Manice LA, Colvin RA, Luster AD Chemokine receptor CXCR3 and its ligands CXCL9 and CXCL10 are required for the development of murine cerebral malaria. Proc. Natl. Acad. Sci. U.S.A., 2008-03-17;105(12):4814-9. 2008-03-17 [PMID: 18347328] (Mouse)
• Fujita H, Asahina A, Komine M, Tamaki K The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Cell. Immunol., 2007-05-15;245(2):70-9. 2007-05-15 [PMID: 17507001] (Mouse)
• Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice. Am. J. Pathol., 2007-04-01;170(4):1152-64. 2007-04-01 [PMID: 17392156] (Mouse)
• Hervas-Stubbs S, Olivier A, Boisgerault F, Thieblemont N, Leclerc C TLR3 ligand stimulates fully functional memory CD8+ T cells in the absence of CD4+ T-cell help. Blood, 2007-03-05;109(12):5318-26. 2007-03-05 [PMID: 17339421] (Mouse)
• Wuest T, Austin BA, Uematsu S, Thapa M, Akira S, Carr DJ Intact TRL 9 and type I interferon signaling pathways are required to augment HSV-1 induced corneal CXCL9 and CXCL10. J. Neuroimmunol., 2006-08-01;179(1):46-52. 2006-08-01 [PMID: 16884784] (Mouse)
• Weighardt H, Mages J, Jusek G, Kaiser-Moore S, Lang R, Holzmann B Organ-specific role of MyD88 for gene regulation during polymicrobial peritonitis. Infect. Immun., 2006-06-01;74(6):3618-32. 2006-06-01 [PMID: 16714594] (Mouse)
• Fulkerson PC, Fischetti CA, Hassman LM, Nikolaidis NM, Rothenberg ME Persistent effects induced by IL-13 in the lung. Am. J. Respir. Cell Mol. Biol., 2006-04-27;35(3):337-46. 2006-04-27 [PMID: 16645178] (Mouse)
• Christensen JE, de Lemos C, Moos T, Christensen JP, Thomsen AR CXCL10 is the key ligand for CXCR3 on CD8+ effector T cells involved in immune surveillance of the lymphocytic choriomeningitis virus-infected central nervous system. J. Immunol., 2006-04-01;176(7):4235-43. 2006-04-01 [PMID: 16547260] (Mouse)
• Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation). Blood, 2005-11-03;107(5):2112-22. 2005-11-03 [PMID: 16269622] (Mouse)
• Jensen KK, Manfra DJ, Grisotto MG, Martin AP, Vassileva G, Kelley K, Schwartz TW, Lira SA The human herpes virus 8-encoded chemokine receptor is required for angioproliferation in a murine model of Kaposi's sarcoma. J. Immunol., 2005-03-15;174(6):3686-94. 2005-03-15 [PMID: 15749907] (Mouse)
• Tanaka M, Terry RD, Mokhtari GK, Inagaki K, Koyanagi T, Kofidis T, Mochly-Rosen D, Robbins RC Suppression of graft coronary artery disease by a brief treatment with a selective epsilonPKC activator and a deltaPKC inhibitor in murine cardiac allografts. Circulation, 2004-09-14;110(11):II194-9. 2004-09-14 [PMID: 15364862] (Mouse)
• Tanaka M, Nakae S, Terry RD, Mokhtari GK, Gunawan F, Balsam LB, Kaneda H, Kofidis T, Tsao PS, Robbins RC Cardiomyocyte-specific Bcl-2 overexpression attenuates ischemia-reperfusion injury, immune response during acute rejection, and graft coronary artery disease. Blood, 2004-07-27;104(12):3789-96. 2004-07-27 [PMID: 15280201] (Mouse)

FAQs for CXCL9/MIG (MCX900). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Cxcl9