MOK Antibody

Western Blot: RAGE Antibody [NBP2-03950] - analysis of RAGE in 1) human lung tissue lysate, mouse lung tissue lysate in the 2) absence and 3) presence of immunizing peptide, and 4) rat lung tissue lysate using this antibody. 1 and 0.5 ug/ml, respectively. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: RAGE Antibody [NBP2-03950] - analysis of RAGE in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.

Product Discontinued

MOK Antibody Summary

• Immunogen: A portion of amino acids 1-60 of human RAGE was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MOK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-3 ug/ml

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for MOK Antibody

• EC 2.7.11.22
• MAPK/MAK/MRK overlapping kinase
• MOK Protein Kinase
• RAGE
• RAGE1
• RAGE-1
• renal cell carcinoma antigen
• Renal tumor antigen 1
• STK30

Background

Advanced glycation end-products receptor (RAGE) is reported to be a multiligand receptor of the immunoglobulin superfamily expressed in myoblasts. It is developmentally regulated in skeletal muscle. It is reported that RAGE along with Amphoterin stimulates myoblast differentiation by the combined effect of stimulation of differentiation and inhibition of proliferation, and deregulation of RAGE expression in myoblasts might contribute to their neoplastic transformation. Various studies report that RAGE signaling in microglia contributes to the pathogenesis of an inflammatory response that ultimately impairs neuronal function and directly affects amyloid accumulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MOK Antibody (NBP2-03950)(3)

Publications using NBP2-03950

• Farini A, Tripodi L, Villa C et al. Inhibition of the immunoproteasome modulates innate immunity to ameliorate muscle pathology of dysferlin-deficient BlAJ mice Cell death & disease 2022-11-19 [PMID: 36402750]
• Evran S, Calis F, Akkaya E et al. The effect of high mobility group box-1 protein on cerebral edema, blood-brain barrier, oxidative stress and apoptosis in an experimental traumatic brain injury model Brain Res. Bull. 2019-11-09 [PMID: 31715313] (IF/IHC, Rat)
• Chen MC, Lin JA, Lin HT, Chen SY et al. Potential effect of advanced glycation end products (AGEs) on spermatogenesis and sperm quality in rodents Food Funct 2019-05-16 [PMID: 31095144]

Bioinformatics

• Gene Symbol: MOK
• Uniprot:
  • NP_031451.2(Mouse)