MMR/CD206/Mannose Receptor Antibody (309210) [Unconjugated]

MMR/CD206 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Rat Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB2534) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 309210
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

MMR/CD206/Mannose Receptor Antibody (309210) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMR
  Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
  Accession # P22897
• Specificity: Detects human MMR in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse MMR.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: MRC1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody (309210) [Unconjugated]

• CD206
• CLEC13D
• CLEC13Dmacrophage mannose receptor 1
• C-type lectin domain family 13 member D
• mannose receptor, C type 1
• MMR
• MMRCD206 antigen
• MRC1

Background

The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca²⁺-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
4. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
5. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
6. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
7. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMR/CD206/Mannose Receptor Antibody (MAB2534)(6)

Publications using MAB2534

• JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis Cell Death & Disease, 2022-06-06;13(6):527. 6/6/2022 [PMID: 35661105]
• P Nejman-Gry, M Papli?ska-, M Proboszcz, M Grabczak, J Hermanowic, R Krenke The expression of IL17RA on sputum macrophages in asthma patients Cytokine, 2021-04-08;0(0):155518. 2021-04-08 [PMID: 33840588] (Human)
• Y Suzuki, M Shirai, K Asada, H Yasui, M Karayama, H Hozumi, K Furuhashi, N Enomoto, T Fujisawa, Y Nakamura, N Inui, T Shirai, H Hayakawa, T Suda Macrophage mannose receptor, CD206, predict prognosis in patients with pulmonary tuberculosis Sci Rep, 2018-09-03;8(1):13129. 2018-09-03 [PMID: 30177769] (IHC, Human)
• Maija Hollmén, Sinem Karaman, Simon Schwager, Angela Lisibach, Ailsa J. Christiansen, Mikael Maksimow et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer OncoImmunology 2016-03-03 [PMID: 27141367] (Immunocytochemistry, Human)
• Zandi S, Nakao S, Chun K, Fiorina P, Sun D, Arita R, Zhao M, Kim E, Schueller O, Campbell S, Taher M, Melhorn M, Schering A, Gatti F, Tezza S, Xie F, Vergani A, Yoshida S, Ishikawa K, Yamaguchi M, Sasaki F, Schmidt-Ullrich R, Hata Y, Enaida H, Yuzawa M, Yokomizo T, Kim Y, Sweetnam P, Ishibashi T, Hafezi-Moghadam A ROCK-isoform-specific polarization of macrophages associated with age-related macular degeneration. Cell Rep, 2015-02-19;10(7):1173-86. 2015-02-19 [PMID: 25704819] (IHC, Human)
• Elizabeth C Co, Matthew Gormley, Mirhan Kapidzic, David B Rosen, Marvin A Scott, Haley A R Stolp et al. Maternal Decidual Macrophages Inhibit NK Cell Killing of Invasive Cytotrophoblasts During Human Pregnancy Biology of Reproduction 2013-06-20 [PMID: 23553431] (Immunohistochemistry, Human)

Review for MMR/CD206/Mannose Receptor Antibody (MAB2534) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 02/19/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 21943944
• Species: Human

Bioinformatics

• Gene Symbol: MRC1
• Uniprot:
  • P22897()