MMP-2 Antibody [Unconjugated]

Western blot shows lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MMP-2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human MMP-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of U‑118‑MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MMP‑2 at approximately 78 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MMP-2 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human MMP‑2
  Ile34-Cys660
  Accession # P08253
• Specificity: Detects human MMP-2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MMP2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-2 Antibody [Unconjugated]

• 72 kDa gelatinase
• CLG4
• CLG4A72 kDa type IV collagenase
• collagenase type IV-A
• EC 3.4.24
• EC 3.4.24.24
• Gelatinase A
• matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
• matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
• Matrix metalloproteinase-2
• matrix metalloproteinase-II
• MMP2
• MMP-2
• MMP-II
• MONA
• neutrophil gelatinase
• TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-2 Antibody (AF902)(23)

Publications using AF902

• Varga, AJ;Nemeth, IB;Kemeny, L;Varga, J;Tiszlavicz, L;Kumar, D;Dodd, S;Simpson, AWM;Buknicz, T;Beynon, R;Simpson, D;Krenacs, T;Dockray, GJ;Varro, A; Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma Cells International journal of molecular sciences 2023-11-28 [PMID: 38069171] (Western Blot, Human)
• Ma?ek, A;Wojnicki, M;Borkowska, A;Wójcik, M;Zió?ek, G;Lechowski, R;Zabielska-Koczyw?s, K; Gold Nanoparticles Inhibit Extravasation of Canine Osteosarcoma Cells in the Ex Ovo Chicken Embryo Chorioallantoic Membrane Model International journal of molecular sciences 2023-06-07 [PMID: 37373007] (Western Blot, Canine)
• EJ Klaver, L Dukes-Rims, B Kumar, ZJ Xia, T Dang, MA Lehrman, P Angel, RR Drake, HH Freeze, R Steet, H Flanagan-S Protease-dependent defects in N-cadherin processing drive PMM2-CDG pathogenesis JCI Insight, 2021-12-22;0(0):. 2021-12-22 [PMID: 34784297] (Western Blot, Zebrafish)
• Kristaps Dambergs, Gunta Sumeraga, Māra Pilmane Complex Evaluation of Tissue Factors in Pediatric Cholesteatoma Children (Basel) 2021-10-16 [PMID: 34682191] (Immunohistochemistry, Human)
• Kristaps Dambergs, Gunta Sumeraga, Māra Pilmane Complex Evaluation of Tissue Factors in Pediatric Cholesteatoma Children (Basel) 10/16/2021 [PMID: 34682191]
• Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1 Cancer Cell Int, 2020-10-02;20(0):476. 2020-10-02 [PMID: 33024414] (Western Blot, Human)
• RM Escalona, M Bilandzic, P Western, E Kadife, G Kannouraki, JK Findlay, N Ahmed TIMP-2 regulates proliferation, invasion and STAT3-mediated cancer stem cell-dependent chemoresistance in ovarian cancer cells BMC Cancer, 2020-10-06;20(1):960. 2020-10-06 [PMID: 33023532] (ICC, Human)
• Aysha Ferdoushi, Xiang Li, Nathan Griffin, Sam Faulkner, M. Fairuz B. Jamaluddin, Fangfang Gao et al. Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis Frontiers in Oncology 8/25/2020 [PMID: 32984024]
• Frew John W, Hawkes Jason E, Krueger James G A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa. F1000Research 6/25/2019 [PMID: 31281635]
• G Ruiz-Gómez, S Vogel, S Möller, MT Pisabarro, U Hempel Glycosaminoglycans influence enzyme activity of MMP2 and MMP2/TIMP3 complex formation - Insights at cellular and molecular level Sci Rep, 2019-03-20;9(1):4905. 2019-03-20 [PMID: 30894640] (ICC, Human)
• E Ugarte-Ber, E Martens, L Boon, J Vandooren, D Blockmans, P Proost, G Opdenakker EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosus J. Cell. Mol. Med., 2018-10-24;23(1):576-585. 2018-10-24 [PMID: 30358100]
• Z Vitenberga, M Pilmane Age-related lung tissue remodeling due to the local distribution of MMP-2, TIMP-2, TGF-? and Hsp70 Biotech Histochem, 2018-01-12;0(0):1-10. 2018-01-12 [PMID: 29325453] (Human)
• Zuzanna Drebert, Elly De Vlieghere, Jolien Bridelance, Olivier De Wever, Karolien De Bosscher, Marc Bracke et al. Glucocorticoids indirectly decrease colon cancer cell proliferation and invasion via effects on cancer-associated fibroblasts Experimental Cell Research 1/1/2018 [PMID: 29196164]
• Samuel Martín-Vílchez, Yolanda Rodríguez-Muñoz, Rosario López-Rodríguez, Ángel Hernández-Bartolomé, María Jesús Borque-Iñurrita, Francisca Molina-Jiménez et al. Inhibition of Tyrosine Kinase Receptor Tie2 Reverts HCV-Induced Hepatic Stellate Cell Activation PLoS ONE 2014-10-10 [PMID: 25302785] (Western Blot, Human)
• Samuel Martín-Vílchez, Yolanda Rodríguez-Muñoz, Rosario López-Rodríguez, Ángel Hernández-Bartolomé, María Jesús Borque-Iñurrita, Francisca Molina-Jiménez et al. Inhibition of Tyrosine Kinase Receptor Tie2 Reverts HCV-Induced Hepatic Stellate Cell Activation PLoS ONE 10/10/2014 [PMID: 25302785]
• Solli AI, Fadnes B, Winberg JO et al. Tissue- and Cell-Specific Co-localization of Intracellular Gelatinolytic Activity and Matrix Metalloproteinase 2. J Histochem Cytochem 6/1/2013 [PMID: 23482328]
• Elga Mozeika, Mara Pilmane, Birgit Meinecke Nürnberg, Gregor B E Jemec Tumour Necrosis Factor-alpha and Matrix Metalloproteinase-2 are Expressed Strongly in Hidradenitis Suppurativa Acta Dermato Venereologica 1/1/2013 [PMID: 23096596]
• Zacharias DG, Kim SG, Massat AE, Bachar AR, Oh YK, Herrmann J, Rodriguez-Porcel M, Cohen P, Lerman LO, Lerman A Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans. PLoS ONE, 2012-02-06;7(2):e31065. 2012-02-06 [PMID: 22328926] (ICC, Human)
• Yong HY, Kim IY, Kim JS, Moon A ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells requires MMP-13 and uPA upregulation via p38 MAPK signaling. Int. J. Oncol., 2010-02-01;36(2):501-7. 2010-02-01 [PMID: 20043086] (Western Blot, Human)
• Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN Fibroblast-conditioned media promote human sarcoma cell invasion. Surgery, 2008-09-14;145(1):42-7. 2008-09-14 [PMID: 19081474] (Western Blot, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)
• Leotlela PD, Wade MS, Duray PH, Rhode MJ, Brown HF, Rosenthal DT, Dissanayake SK, Earley R, Indig FE, Nickoloff BJ, Taub DD, Kallioniemi OP, Meltzer P, Morin PJ, Weeraratna AT Claudin-1 overexpression in melanoma is regulated by PKC and contributes to melanoma cell motility. Oncogene, 2006-12-11;26(26):3846-56. 2006-12-11 [PMID: 17160014] (ICC, Human)
• Berceli SA, Jiang Z, Klingman NV, Pfahnl CL, Abouhamze ZS, Frase CD, Schultz GS, Ozaki CK Differential expression and activity of matrix metalloproteinases during flow-modulated vein graft remodeling. J. Vasc. Surg., 2004-05-01;39(5):1084-90. 2004-05-01 [PMID: 15111865] (IHC-P, Rabbit)

Bioinformatics

• Gene Symbol: MMP2
• Uniprot:
  • P08253()