MMP-12 ELISA Development Kit

Product Discontinued

MMP-12 ELISA Development Kit Summary

• Standard Curve Range: 0.008 - 1 ug/ml
• Sensitivity: <50pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Kit Type: ELISA Development Kit
• Gene: MMP12

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: The human HME-MMP12 ELISA kit is for quantitative determination of HME-MMP12 concentrations in human cell culture supernatants and biological fluids (plasma, serum, sputum). This kit is for research purpose only. Kit contents Capture Antibody: 0.5mg/mL of mouse anti-HME-MMP12 monoclonal antibody (clone 701E4.03). Detection Antibody: 0.5mg/mL of HRPO-conjugated anti-HME-MMP12 mouse monoclonal antibody (clone 706F9.01). Standard: Each vial contains a solution of recombinant HME-MMP12 (concentration indicated on the vial) produced, purified and concentrated from transfected eukaryotic cells. A 8 point standard curve using 2-fold serial dilutions in sample dilution buffer, starting at a concentration of 1ug/ml is recommended.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for MMP-12 ELISA Development Kit

• EC 3.4.24
• hME
• HMEEC 3.4.24.65
• Macrophage elastase
• macrophage metalloelastase
• matrix metallopeptidase 12 (macrophage elastase)
• matrix metalloproteinase 12 (macrophage elastase)
• Matrix metalloproteinase-12
• ME
• MGC138506
• MME
• MMP12
• MMP-12

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. HME/MMP-12, also called metalloelastase, is critical for invasion and destruction in pathologies such as aneurysm and emphysema. . HME mRNA and protein were previously described as expressed in human alveolar macrophages (Curci JA et al, 1998, J Clin Invest, 102(11):1900-10). The predicted molecular mass of the HME proenzyme is 54 kDa and readily undergoes NH2- and COOH-terminal processing to an active 45KDa form and a mature 22 kDa form. HME is a unique human metalloproteinase that displays elastolytic activity. We have isolated HME-MMP12 cDNA from a human CD34+-derived DC (GM-CSF+TNFa) library. Expression analysis revealed HME-MMP12 expression in lung and placenta. Anti-HME-MMP12 monoclonal antibodies were obtained after mice immunization with HME-MMP12-transfected eukaryotic cells. These antibodies were used to set up a quantitative immunoassay for the detection of human HME-MMP12 (Demedts IK et al, 2006; Thorax, 61:196-201; Lapan et al, BMC Pulmonary Medicine, 2010, 10:40).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for MMP-12 Kit (DDXK-E-MMP12-2)(2)

Publications using DDXK-E-MMP12-2

• Lapan P et al. Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum. BMC Pulm Med 2010-08-02 [PMID: 20678199]
• Demets IK et al. Elevated MMP-12 protein levels in induced sputum from patients with COPD. Thorax 2006-03-01 [PMID: 16308335]

Bioinformatics

• Gene Symbol: MMP12