MMP-1 Antibody (6A5)

Images

 
Western Blot: MMP-1 Antibody (6A5) [NBP2-22123] - Western blot analysis using MMP1 mAb against HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: MMP-1 Antibody (6A5) [NBP2-22123] - Immunofluorescence analysis of Hela cells using MMP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: MMP-1 Antibody (6A5) [NBP2-22123] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using MMP1 mouse mAb with ...read more
Flow Cytometry: MMP-1 Antibody (6A5) [NBP2-22123] - Flow cytometric analysis of Hela cells using MMP1 mouse mAb (green) and negative control (purple).
ELISA: MMP-1 Antibody (6A5) [NBP2-22123] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
6A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MMP-1 Antibody (6A5) Summary

Immunogen
Purified recombinant fragment of human MMP1 expressed in E. coli. [UniProt# P03956]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This MMP1 (6A5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-22123 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Human and mouse. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for MMP-1 Antibody (6A5)

  • CLGmatrix metalloprotease 1
  • CLGN
  • EC 3.4.24
  • EC 3.4.24.7
  • Fibroblast collagenase
  • interstitial collagenase
  • matrix metallopeptidase 1 (interstitial collagenase)
  • matrix metalloproteinase 1 (interstitial collagenase)
  • Matrix metalloproteinase-1
  • MMP1
  • MMP-1

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-1 Antibody (NBP2-22123)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Human
(1)
All Species
Showing Publication 1 - 1 of 1.
Publication using NBP2-22123 Applications Species
Kim MW, Park JT, Kim JH et al. Periostin in Mature Stage Localized Scleroderma.. Ann Dermatol Jun 1 2017 [PMID: 28566901] (IHC, Human) IHC Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP1
Entrez
Uniprot