MMP-1 Antibodies: Novus Biologicals

MAB901

MMP-1 Antibody (36665) [Unconjugated]

200 μg

MAB901

HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # <a class=

CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of PC-3 human prostate cancer parental cell line and MMP-1 knockout PC-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Inhibition of PKC alpha decreases the protein expression of MMP‐1 and MMP‐9 induced by cell cross‐talk in SMC. A, Evaluation of PKC alpha activation in control SMC (S NG) vs SMC previously co‐cultured with MAC in HG (Sc HG). Note that, the phosphorylated form of PKC alpha is significantly increased in SMC that were before exposed to MAC in HG conditions. Silencing of CCR2 or p65 decreases the phosphorylation of PKC alpha to control levels. B, Effect of PKC alpha inhibitor – Go 6976 on the MMP‐1 and MMP‐9 protein expression induced by SMC‐MAC cross‐talk. S NG – control SMC, Sc NG – SMC which were co‐cultured with MAC in normal glucose, Sc HG ‐ SMC which were co‐cultured with MAC in high glucose, Sc HGGo ‐ SMC which were co‐cultured with MAC in high glucose in presence of 1 μmol/L Go 6976. n = 3, *P < .05, **P < .01, #P < .05, ##P < .01 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29992758), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DDC up-regulates MMP-1 through ERK1/2 and Akt activation(A) Co-cultures were treated with 100 μM DDC for the indicated time. Phosphorylation of ERK1/2, p38 and Akt were determined by Western blotting analysis. The corresponding non-phosphorylated ERK1/2, p38, Akt and beta -actin were used for protein loading control. (B) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of ERK1/2 inhibitor (U0126, 10 μM). MMP-1 protein levels were analysed by Western blotting. (C) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of p38 inhibitor (SB203580, 10 μM). MMP-1 protein levels were analysed by Western blotting. (D) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of Akt inhibitor (T3830, 50 μM). MMP-1 protein levels were analysed by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DDC up-regulates MMP-1 expression in a dose-dependent manner and lowered collagen I in LX-2 cellsCo-culture systems were treated with 25, 50, 100 and 200 μM DDC for 24 h. LX-2 cells and the culture medium were collected and analysed. (A) Quantitative RT-PCR results of MMP-1 expression in LX-2 cells. The results were normalized with beta -actin and expressed as fold increase relative to the control in each experiment. (B) 20 μg aliquots of total protein extracts from LX-2 cells were subjected to Western blotting analysis with anti-MMP-1 antibody (MAB 901 and MAB 3223 for active form only) and beta -actin antibody as a loading control, as described in the Materials and Methods section. (C) 30 μg of LX-2 lysates or 20 μl aliquots of 10-fold concentrated culture medium with 5×loading buffer were assayed by Casein Zymography for MMP-1 activity. (D) Levels of collagen I in LX-2 cells were assayed by western blotting. (E) Levels of collagen I in the culture medium were assayed by ELISA. *P<0.05 compared with untreated C3A-2E1/LX-2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Co‐culture of SMC and MAC in high glucose conditions (24 h) induces a significant increase in the protein expression of MMP‐1 and MMP‐9. A, B: Protein expression of MMP‐1 (A) and MMP‐9 (B) was assessed in SMC after co‐culture with THP‐derived macrophages and evaluated by Western blot assay. C, D: Protein expression of MMP‐1 (C) and MMP‐9 (D) in SMC after co‐culture with human macrophages derived from freshly isolated monocytes from control subjects (CM) or diabetic patients with ACS (DM). S NG –control SMC, S HG – SMC exposed to HG, Sc NG or Sc CM – SMC which were co‐cultured with MAC in normal glucose, Sc HG or Sc DM ‐ SMC which were co‐cultured with MAC in high glucose. n = 3, *P < .05, **P < .01, ***P < .001, #P < .05, ##P < .01, ###P < .001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29992758), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of CCR2 and p65 silencing in the regulation of MMP‐1 and MMP‐9 expression in SMC. Protein expression of MMP‐1 (A) and MMP‐9 (B) determined by Western blot in control SMC (S NG), SMC which were co‐cultured with MAC in HG conditions (Sc HG), and SMC subjected to silencing of CCR‐2 and p65 and co‐cultured with MAC in HG conditions (Cneg, p65, CCR2 siRNA). Note that silencing of CCR2 or p65 decreases significantly the protein expression of MMP‐1 and MMP‐9 induced by SMC‐MAC co‐culture in high glucose conditions. n = 3, *P < .05, #P < .05 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29992758), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DDC up-regulates MMP-1 expression in an H2O2-associated mannerCo-cultures were treated with 100 μM DDC, 2000 Units catalase, 25 μM vitamin E and 5μM H2O2 combined with 100 μM DDC for 24 h. Cell lysates from the LX-2 cells were collected and analysed. (A) 20 μg aliquots of total protein extracts from LX-2 cells were subjected to Western blotting analysis with anti-MMP-1 antibody (MAB 901) and beta -actin antibody as a loading control, as described in the Materials and Methods section. (B) Levels of collagen I in LX-2 cells were assayed by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DDC up-regulates MMP-1 expression in LX-2 cellsCo-culture systems were treated with 100 μM DDC for 24 h. LX-2 cells and the culture medium were collected and analysed. (A) Quantitative RT-PCR results of MMP-1 expression in LX-2 cells. The results were normalized with beta -actin and expressed as fold increase relative to the control in each experiment. (B) 20 μg aliquots of total protein extracts from LX-2 cells were subjected to western blotting analysis with anti-MMP-1 antibody (MAB 901) and beta -actin antibody as a loading control, as described in the Materials and Methods section. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

MMP-1, MMP-3, and MMP-13 expression. (A-D) MMP-1; (E-H) MMP-3; (I-L) MMP-13 (A,E,I) ACL from young normal knee; (B,F,J) ACL from aging knee; (C,G,K) fibroblast-like cell aggregates in the degenerated ACL; (D,H,L) chondrocyte-like cell aggregates in the degenerated ACL. (Original magnification ×40). The graph (M) represents the percentage (mean ± SEM) of MMP-1-, MMP-3-, and MMP-13-positive cells in each group. **P < 0.01. (N) Western blotting for MMP-1, MMP-3, and MMP-13 (n = 3 for each category). Image collected and cropped by CiteAb from the following open publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar4165), licensed under a CC-BY license. Not internally tested by R&D Systems.

AEG-1 knockdown down-regulated MMP1 expression in HNSCC cells in vitro and in vivo. (A) microarray analysis of gene expression after AEG-1 knockdown in SAS cells. Genes with an absolute fold change greater than 2.5 are shown. SNORD3B-1, Homo sapiens small nucleolar RNA, C/D box 3B-1; EMILIN2, elastin microfibril interfacer 2; FABP4, fatty acid binding protein 4; MMP1, matrix metallopeptidase 1; ANO1, anoctamin 1; RELN, reelin; FPR2, formyl peptide receptor 2; UHMK1, U2AF homology motif kinase 1; RMND5A, required for meiotic nuclear division 5 homolog A; CAPZA1; capping protein (actin filament) muscle Z-line, alpha 1. *, p < 0.05; **, p < 0.01; ***, p < 0.001. (B) RT-QPCR confirmation of gene expression profiles after knockdown of AEG-1 in SAS and FaDu cells. All experiments were performed in triplicate (n = 3) and data were normalized to GAPDH. (C) secreted MMP1 protein in cell-conditioned culture media. Each cell lines were seeded in equal numbers (1 × 106 cells) and were cultured in starving condition (1% fetal bovine serum, FBS) for 24 hr and the media were harvested. (D) immunohistochemical staining of MMP1 in murine subcutaneous xenograft tumors (upper row) and metastatic lesions from in vivo lung metastasis assays (lower row). Scale bar: upper row, 100 μm; lower row, 25 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24063540), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of MMP1 on gene and protein levels upon treatment with mitomycin C (MMC) of SKOV3 cells in normoxia and hypoxia. MMP1 gene expression was measured using (A) HTA 2.0 microarrays and (B) RT-qPCR [normalized to PPIA and GUSB levels and normoxia control (NC) as 1]. (C) Immunoblot of MMP 1 protein (with vinculin as loading control). (D) Quantification of MMP1 protein levels by densitometry. (E) Secretion of pro-MMP1 protein levels measured by ELISA.(F) Secretion of total MMP1 protein levels measured by ELISA. (B,D) Results were normalized to normoxia control as 1. ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05; VIN, vinculin. A detailed statistics description is provided in the Supplementary Table S2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40584967), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB901U-100UG
Alexa Fluor 405	FAB901V-100UG
Alexa Fluor 488	FAB901G-100UG
Alexa Fluor 532	FAB901X-100UG
Alexa Fluor 594	FAB901T-100UG
Alexa Fluor 647	FAB901R-100UG
Alexa Fluor 700	FAB901N-100UG
Alexa Fluor 750	FAB901S-100UG
Alexa Fluor Plus 405	FAB901AFP405-100UG
Alexa Fluor Plus 488	FAB901AFP488-100UG
Alexa Fluor Plus 555	FAB901AFP555-100UG
Alexa Fluor Plus 594	FAB901AFP594-100UG
Alexa Fluor Plus 647	FAB901AFP647-100UG
Alexa Fluor Plus 680	FAB901AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB901-SP

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NBP2-22123

MMP-1 Antibody (6A5) - BSA Free

(5 Publications)

0.1 ml

NBP2-22123

Immunohistochemistry-Paraffin: MMP-1 Antibody (6A5) [NBP2-22123] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using MMP1 mouse mAb with DAB staining.

Flow Cytometry: MMP-1 Antibody (6A5) [NBP2-22123] - Flow cytometric analysis of Hela cells using MMP1 mouse mAb (green) and negative control (purple).

ELISA: MMP-1 Antibody (6A5) [NBP2-22123] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22123AF350
Alexa Fluor 405	NBP2-22123AF405
Alexa Fluor 488	NBP2-22123AF488
Alexa Fluor 532	NBP2-22123AF532
Alexa Fluor 594	NBP2-22123AF594
Alexa Fluor 647	NBP2-22123AF647
Alexa Fluor 700	NBP2-22123AF700
Alexa Fluor 750	NBP2-22123AF750
Alexa Fluor Plus 405	NBP2-22123AFP405
Alexa Fluor Plus 488	NBP2-22123AFP488
Alexa Fluor Plus 555	NBP2-22123AFP555
Alexa Fluor Plus 594	NBP2-22123AFP594
Alexa Fluor Plus 647	NBP2-22123AFP647
Alexa Fluor Plus 680	NBP2-22123AFP680
Allophycocyanin	NBP2-22123APC
Allophycocyanin/Cy7	NBP2-22123APCCY7
Biotin	NBP2-22123B
CoraFluor 1	NBP2-22123CL1
DyLight 350	NBP2-22123UV
DyLight 405	NBP2-22123V
DyLight 488	NBP2-22123G
DyLight 550	NBP2-22123R
DyLight 594	NBP2-22123DL594
DyLight 650	NBP2-22123C
DyLight 680	NBP2-22123FR
DyLight 755	NBP2-22123IR
FITC	NBP2-22123F
HRP	NBP2-22123H
Janelia Fluor 525	NBP2-22123JF525
Janelia Fluor 549	NBP2-22123JF549
Janelia Fluor 646	NBP2-22123JF646
Janelia Fluor 669	NBP2-22123JF669
PE	NBP2-22123PE
PE/Atto594	NBP2-22123PEATT594
PE/Cy5.5	NBP2-22123PECY55
PE/Cy7	NBP2-22123PECY7
PerCP	NBP2-22123PCP
mFluor Violet 450 SE	NBP2-22123MFV450
mFluor Violet 500 SE	NBP2-22123MFV500
mFluor Violet 610 SE	NBP2-22123MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22123SS
Azide and BSA Free	NBP2-80852

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AF901

MMP-1 Antibody [Unconjugated]

(10 Publications)

100 μg

AF901

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of PC-3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of PC-3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Left:</b> Simple Western lane view shows lysates of MDA‑MB‑231 human breast cancer cell line, loaded at 0.1 mg/ml. A specific band was detected for MMP‑1 at approximately 55 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 043-522-2). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. <b>Right:</b> Simple Western electropherogram showing the same Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the MDA‑MB‑231 human breast cancer cell line.

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-1 was detected in immersion fixed paraffin-embedded sections of human prostate tissue using Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF901U-100UG
Alexa Fluor 405	AF901V-100UG
Alexa Fluor 488	AF901G-100UG
Alexa Fluor 532	AF901X-100UG
Alexa Fluor 594	AF901T-100UG
Alexa Fluor 647	AF901R-100UG
Alexa Fluor 700	AF901N-100UG
Alexa Fluor 750	AF901S-100UG
Alexa Fluor Plus 405	AF901AFP405-100UG
Alexa Fluor Plus 488	AF901AFP488-100UG
Alexa Fluor Plus 555	AF901AFP555-100UG
Alexa Fluor Plus 594	AF901AFP594-100UG
Alexa Fluor Plus 647	AF901AFP647-100UG
Alexa Fluor Plus 680	AF901AFP680-100UG
Biotin	BAF901

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF901-SP

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MAB9011

MMP-1 Antibody (40013F) [Unconjugated]

100 μg

MAB9011

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB9011) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB9011) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Monoclonal

Host: Goat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9011U-100UG
Alexa Fluor 405	FAB9011V-100UG
Alexa Fluor 488	FAB9011G-100UG
Alexa Fluor 532	FAB9011X-100UG
Alexa Fluor 594	FAB9011T-100UG
Alexa Fluor 647	FAB9011R-100UG
Alexa Fluor 700	FAB9011N-100UG
Alexa Fluor 750	FAB9011S-100UG
Alexa Fluor Plus 405	FAB9011AFP405-100UG
Alexa Fluor Plus 488	FAB9011AFP488-100UG
Alexa Fluor Plus 555	FAB9011AFP555-100UG
Alexa Fluor Plus 594	FAB9011AFP594-100UG
Alexa Fluor Plus 647	FAB9011AFP647-100UG
Alexa Fluor Plus 680	FAB9011AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB9011-SP

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H00004312-D01P

MMP-1 Antibody - Azide and BSA Free

0.1 mg

H00004312-D01P

Western Blot: MMP-1 Antibody [H00004312-D01P] - Analysis of MMP1 expression in transfected 293T cell line by MMP1 polyclonal antibody.Lane 1: MMP1 transfected lysate(54.00 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: MMP-1 Antibody [H00004312-D01P] - Analysis of protein-protein interactions between MMP1 and F2R. HeLa cells were stained with anti-MMP1 rabbit purified polyclonal 1:1200 and anti-F2R mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-21660

MMP-1 Antibody (SR1958) - BSA Free

100 ul

NBP3-21660

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21660-50ul

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NBP2-55118

MMP-1 Antibody - BSA Free

100 ul

NBP2-55118

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-55118-25ul

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NBP3-22010

MMP-1 Antibody (SR1951) - BSA Free

100 ul

NBP3-22010

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-22010-50ul

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MAB901R

MMP-1 Antibody (36665R) [Unconjugated]

(1 Publication)

100 μg

MAB901R

HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of IMR-90 human lung fibroblast cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Recombinant Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of IMR-90 human lung fibroblast cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Recombinant Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-1 was detected in immersion fixed paraffin-embedded sections of human liver using Recombinant Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB901RU-100UG
Alexa Fluor 405	FAB901RV-100UG
Alexa Fluor 488	FAB901RG-100UG
Alexa Fluor 532	FAB901RX-100UG
Alexa Fluor 594	FAB901RT-100UG
Alexa Fluor 647	FAB901RR-100UG
Alexa Fluor 700	FAB901RN-100UG
Alexa Fluor 750	FAB901RS-100UG
Alexa Fluor Plus 405	FAB901RAFP405-100UG
Alexa Fluor Plus 488	FAB901RAFP488-100UG
Alexa Fluor Plus 555	FAB901RAFP555-100UG
Alexa Fluor Plus 594	FAB901RAFP594-100UG
Alexa Fluor Plus 647	FAB901RAFP647-100UG
Alexa Fluor Plus 680	FAB901RAFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB901R-SP
Carrier Free	MAB901R-500
Carrier Free	MAB901R-01M

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IC9011P

MMP-1 Antibody (36607) [Phycoerythrin]

(2 Publications)

100 Tests

IC9011P

IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="PC-3 human prostate cancer cell line was stained with Mouse Anti-Human MMP-1 PE-conjugated Monoclonal Antibody (Catalog # IC9011P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Phycoerythrin

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FAB901AFP405

MMP-1 Antibody (36665) [Alexa Fluor™ Plus 405]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 405

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FAB901RAFP680

MMP-1 Antibody (36665R) [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 680

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

MMP-1 Antibodies

MMP-1 Antibody (36665) [Unconjugated]

MMP-1 Antibody (6A5) - BSA Free

MMP-1 Antibody [Unconjugated]

MMP-1 Antibody (40013F) [Unconjugated]

MMP-1 Antibody - Azide and BSA Free

MMP-1 Antibody (SR1958) - BSA Free

MMP-1 Antibody - BSA Free

MMP-1 Antibody (SR1951) - BSA Free

MMP-1 Antibody (36665R) [Unconjugated]

MMP-1 Antibody (36607) [Phycoerythrin]

MMP-1 Antibody (36665) [Alexa Fluor™ Plus 405]

MMP-1 Antibody (36665R) [Alexa Fluor™ Plus 680]