Recombinant Human MLF1 Interacting Protein His Protein

SDS-Page: Recombinant Human MLF1 Interacting Protein His Protein [NBP2-23247] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human MLF1 Interacting Protein His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 147-418 of Human MLF1 Interacting Protein
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRN AAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CENPU
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 33.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human MLF1 Interacting Protein His Protein

• CENP-50
• CENP-U
• CENPU50
• CENPUMLF1-interacting protein
• centromere protein of 50 kDa
• centromere protein U
• FLJ23468
• ICEN24
• Interphase centromere complex protein 24
• KLIP1CENP50
• KSHV latent nuclear antigen interacting protein 1
• KSHV latent nuclear antigen-interacting protein 1
• MLF1 interacting protein
• PBIP1
• Polo-box-interacting protein 1

Background

MLF1IP, as known as centromere, is a specialized chromatin domain, present throughout the cell cycle that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. It plays an important role in the correct PLK1 localization to the mitotic kinetochores. It is a scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. It also involved in transcriptional repression. Recombinant human MLF1IP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CENPU