Mitofusin 1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mfn-1 expressed in mouse neural stem cells. Image from verified customer review.
Immunohistochemistry: Mitofusin 1 Antibody [NBP1-51841] - IHC staining of Mfn1 in mouse intestine.
Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mitofusin-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.15 mg/ml

Mitofusin 1 Antibody Summary

Immunogen
A genomic peptide made to an N-terminal region of human Mitofusin-1 (within residues 5-100). [Swiss-Prot Q8IWA4]
Localization
Mitochondrion outer membrane; Multi-pass membrane protein.
Marker
Mitochondrial Fusion Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MFN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This MFN1 antibody is useful for Immunocytochemistry/Immunofluorescence, and IHC-P. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Western Blot was reported in scientific literature.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-51841 in the following applications:

Publications
Read Publications using
NBP1-51841 in the following applications:

  • WB
    4 publications

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.15 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Mitofusin 1 Antibody

  • DKFZp762F247
  • EC 3.6.5
  • EC 3.6.5.-
  • FLJ20693
  • Fzo homolog
  • hfzo1
  • hfzo2
  • MFN1
  • MGC41806
  • mitochondrial transmembrane GTPase Fzo-1
  • mitochondrial transmembrane GTPase FZO-2
  • Mitofusin 1
  • mitofusin-1
  • putative transmembrane GTPase
  • Transmembrane GTPase MFN1

Background

Mitochondria are dynamic organelles that continuously divide and fuse, and their dynamics/distribution is critical for their role in bioenergetics and cell survival. Mitochondrial fusion process is mediated by mitofusin 1 (MFN1) and mitofusin 2 (MFN2) proteins in the outer membrane as well as OPA1 in the inner mitochondrial membrane. MFN1 is an essential transmembrane GTPase critical to mitochondrial fusion; acts independently of the cytoskeleton, and its overexpression induces formation of mitochondrial networks. MFN1 forms homo-/-heteromultimers (with MFN2), and the multimerization has been proposed to be essential for mitochondrion fusion. MFN1 interacts with VAT1 and is ubiquitinated by MARCH5. A mutation in the GTPase domain disrupts the mitochondrial fusion activity of MFN1/MFN2 and loss of MFN1/2 leads to mitochondrial dysfunction mediated embryonic lethality in mice. MFN proteins have been reported to regulate mitochondrial membrane potential, while silencing of MFN1/2 resulte in mitochondrial fragmentation and increased apoptotic sensitivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mitofusin 1 Antibody (NBP1-51841)(4)

Review for Mitofusin 1 Antibody (NBP1-51841) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-51841:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Mitofusin 1 NBP1-51841
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reviewed by:
Vasundhra Bahl
ICC Mouse 03/28/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedParaformeldehyde Fixed Cells
SpeciesMouse

Blocking

Blocking DetailsBlocked with 5% horse serum for 1 hour at room temperature

Primary Anitbody

Dilution Ratiodilution 1:50 at 4 degree celcius overnight

Secondary Antibody

Secondary DescriptionFITC labelled goat anti rabbit

Details

Detection NotesNikon Ti Eclipse microscope
Fixation Details4% paraformaldehyde fixed cells (15 minutes room temperature)
Wash Descriptionwashed with PBS 5 times for 5 minutes each

Product General Protocols

View specific protocols for Mitofusin 1 Antibody (NBP1-51841): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mitofusin 1 Antibody (NBP1-51841) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-51841AF405
Alexa Fluor 488 Labeled NBP1-51841AF488
Alexa Fluor 647 Labeled NBP1-51841AF647
Alexa Fluor 700 Labeled NBP1-51841AF700
Biotin Labeled NBP1-51841B
DyLight 405 Labeled NBP1-51841V
DyLight 488 Labeled NBP1-51841G
DyLight 550 Labeled NBP1-51841R
DyLight 650 Labeled NBP1-51841C
FITC Labeled NBP1-51841F
HRP Labeled NBP1-51841H

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Recent Reviews

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Vasundhra Bahl
03/28/2014
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol MFN1
Entrez
Uniprot