Mitofilin Antibody - BSA Free

Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.

Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NB100-1919] - HeLa cells with antibody 1:500 dilution labeling mitochondria.

Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. The representative image shows a specific and expected granular staining of Mitofilin in the keratinocytes as well as the other cells of epidermal layer with no staining in basal cells. [60X Magnification]

Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import-defective. HEK293 cells that transiently expressed wild type or mutant COA7HIS were solubilized, and the affinity purification of COA7HIS was performed. The samples were analyzed by reducing and non-reducing SDS-PAGE and Western blot. Load: 3%. Eluate: 100%. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201809561), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P analysis of Mitofilin/Mitochondrial inner membrane protein in a transverse section of normal human skeletal muscle using Mitofilin antibody at 1:200 dilution. The myocytes showed an expected and very specific granular staining in the cytoplasm. [10X Magnification]

Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. Specific cytoplasmic positivity of Mitofilin was observed in the keratinocytes as well as other cells of the epidermal layer except for basal cells and dermal connective tissue. [10X Magnification]

Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import‐defectiveASchematic representation of wild‐type & mutant COA7.BCellular protein extracts were isolated from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The samples were analyzed by reducing SDS–PAGE & Western blot.CCellular fractions were prepared from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The fractions were analyzed by reducing SDS–PAGE & Western blot. T, total; C, cytosol; M, mitochondria.DHEK293 cells that transiently expressed wild‐type or mutant COA7HIS were solubilized, & the affinity purification of COA7HIS was performed. The samples were analyzed by reducing & non‐reducing SDS–PAGE & Western blot. Load: 3%. Eluate: 100%.EEqual amounts of wild‐type & mutant radiolabeled [35S]COA7 precursors were imported into mitochondria isolated from HEK293 cells. The samples were analyzed by reducing SDS–PAGE & autoradiography. The results of three biological replicates were analyzed, quantified, & normalized to wild‐type COA7 at 45 min. The data are expressed as a mean ± SEM (n = 3). IAA, iodoacetamide.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30885959), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ca, Fe, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Mitofilin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Mitofilin Antibody - BSA Free (NB100-1919) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Mitofilin Antibody: Cited in 17 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Whole human recombinant Mitofilin [UniProt# Q16891]
• Localization: Mitochondria
• Marker: Mitochondria Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IMMT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation
  • Western Blot 1:2000
• Application Notes: This Mitofilin antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where two bands can be seen at approximately 87-90 kDa.
• Control:

  Mitofilin Overexpression Lysate

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33761951). Rat reactivity reported in scientific literature (PMID: 26729625)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Mitofilin Antibody - BSA Free

• DKFZp779P1653
• inner membrane protein, mitochondrial
• MGC111146
• Mic 60
• Mic60
• MICOS Complex Subunit MIC60
• mitochondrial (mitofilin)
• mitofilin
• motor protein
• p87/89
• proliferation-inducing gene 4

Background

Mitofilin is one of the most abundant mitochondrial proteins in the human heart. It appears to play an important role in the maintenance of cristae morphology. Mitofilin was originally described as heart muscle protein (HMP) because of its high expression in the heart. Mitochondria are the center of cellular energy production and essential metabolic reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mitofilin Antibody (NB100-1919)(17)

Publications using NB100-1919

• Chojnacka KJ, Elancheliyan P, Mussulini BHM et al. Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria Molecular biology of the cell 2022-04-01 [PMID: 35080992]
• Abudu YP, Shrestha BK, Zhang W et al. SAMM50 acts with p62 in piecemeal basal- and OXPHOS-induced mitophagy of SAM and MICOS components The Journal of cell biology 2021-08-02 [PMID: 34037656]
• Naia L, Pinho CM, Dentoni G et al. Neuronal cell-based high-throughput screen for enhancers of mitochondrial function reveals luteolin as a modulator of mitochondria-endoplasmic reticulum coupling BMC biology 2021-03-24 [PMID: 33761951] (WB, Mouse)
• Qiu X, Jiang S, Xiao Y, et al. SOX2-dependent expression of dihydroorotate dehydrogenase regulates oral squamous cell carcinoma cell proliferation International journal of oral science 2021-01-29 [PMID: 33510132] (WB, Human)
• Suh BK, Lee SA, Park C, et al. Schizophrenia-associated dysbindin modulates axonal mitochondrial movement in cooperation with p150glued Molecular brain 2021-01-18 [PMID: 33461576] (WB, Mouse)
• Yoshida T, Das NA, Carpenter AJ et al. Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death Cell. Signal. 2020-06-15 [PMID: 32553549] (WB, Mouse)
• Gomez RC, Wawro P, Lis P et al. Membrane association but not identity is required for LRRK2 activation and phosphorylation of Rab GTPases J. Cell Biol. 2019-10-17 [PMID: 31624137]
• Mohanraj K, Wasilewski M, Beninca C et al. Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7 EMBO Mol Med 2019-03-18 [PMID: 30885959] (WB, Human)
• Vukotic M, Nolte H, Konig T et al. Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. Mol Cell 2017-07-14 [PMID: 28712724] (Human)
• Diokmetzidou A, Soumaka E, Kloukina I et al. Desmin and aB-crystallin interplay in maintenance of mitochondrial homeostasis and cardiomyocyte survival. J. Cell. Sci. 2016-08-26 [PMID: 27566162] (WB)
• Wrobel L, Sokol AM, Chojnacka M, Chacinska A The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly. Sci Rep 2016-06-06 [PMID: 27265872] (WB)
• Nguyen NT, Now H, Kim WJ et al. Ubiquitin-like modifier FAT10 attenuates RIG-I mediated antiviral signaling by segregating activated RIG-I from its signaling platform Sci Rep 2016-03-21 [PMID: 26996158] (WB, Human)
• Li L, Gao G, Shankar J et al. p38 MAP Kinase-dependent phosphorylation of the Gp78 E3 ubiquitin ligase controls ER-mitochondria association and mitochondria motility. Mol. Biol. Cell 2015-09-02 [PMID: 26337390] (ICC/IF, Primate)
• Park SJ, Jeong J, Park YU et al. Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics Sci Rep 2015-03-03 [PMID: 25732993] (WB, Human)
• Catalina-Rodriguez O, Kolukula VK, Tomita Y et al. The mitochondrial citrate transporter, CIC, is essential for mitochondrial homeostasis. Oncotarget 2012-10-01 [PMID: 23100451]
• Yang RF, Zhao GW, Liang ST et al. Mitofilin regulates cytochrome c release during apoptosis by controlling mitochondrial cristae remodeling Biochem Biophys Res Commun 2012-10-08 [PMID: 23058921] (WB, Human)
• Odgren, PR et al. Molecular characterization of mitofilin (HMP), a mitochondria-associated protein with predicted coiled coil and intermembrane space targeting domains. J Cell Sci;109 ( Pt 9):2253-64. 1996-09-01 [PMID: 8886976] (ICC/IF, WB, Human)

Reviews for Mitofilin Antibody (NB100-1919) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Julian Wong Simple Western Rat 08/18/2017

Summary

• Application: Simple Western
• Sample Tested: cell line, whole cell lysate
• Species: Rat
• Lot: C-1

reviewed by: Kathleen Emanuel WB Human 06/14/2016

Summary

• Application: Western Blot
• Sample Tested: Protein lysate from mitochondria isolated from SH-SY5Y cells
• Species: Human
• Lot: B-1

reviewed by: Antigoni Diokmetzidou IP Mouse 08/27/2013

Summary

• Application: Immunoprecipitation
• Sample Tested: Mouse heart
• Species: Mouse
• Lot: A4

FAQs for Mitofilin Antibody (NB100-1919). (Showing 1 - 1 of 1 FAQs).

1. One of our customers is interested in testing your Anti-Mitofilin antibody (NB100-1919) with IHC. Do you know how likely this Ab will be in succeeding to this attempt?
   • This Ab has been validated for use in IHC-P in Canine, Feline, Mouse, Rat and Human.

Bioinformatics

• Gene Symbol: IMMT
• Entrez:
  • 10989(Human)
  • 76614(Mouse)
  • 312444(Rat)
• Uniprot:
  • Q16891(Human)