Mitofilin Antibody

Images

 
Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis total protein extract 1:2000 dilution of antibody.
Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody [NB100-1919] - HeLa cells with antibody 1:500 dilution labeling mitochondria.
Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. The ...read more
Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P analysis of Mitofilin/Mitochondrial inner membrane protein in a transverse section of normal human skeletal muscle using Mitofilin antibody at 1:200 ...read more
Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. Specific ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, FeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Mitofilin Antibody Summary

Immunogen
Whole human recombinant Mitofilin [UniProt# Q16891]
Localization
Mitochondria
Marker
Mitochondria Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IMMT
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation
Application Notes
This Mitofilin antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where two bands can be seen at approximately 87-90 kDa.
Positive Control
Mitofilin Lysate (NBL1-11979)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB100-1919 in the following applications:

Publications
Read Publications using
NB100-1919 in the following applications:

  • 2 publications
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Mitofilin Antibody

  • DKFZp779P1653
  • inner membrane protein, mitochondrial
  • MGC111146
  • Mic 60
  • Mic60
  • MICOS Complex Subunit MIC60
  • mitochondrial (mitofilin)
  • mitofilin
  • motor protein
  • p87/89
  • proliferation-inducing gene 4

Background

Mitofilin is one of the most abundant mitochondrial proteins in the human heart. It appears to play an important role in the maintenance of cristae morphology. Mitofilin was originally described as heart muscle protein (HMP) because of its high expression in the heart. Mitochondria are the center of cellular energy production and essential metabolic reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mitofilin Antibody (NB100-1919)(9)

We have publications tested in 2 confirmed species: Human, Primate.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(6)
All Applications
Filter By Species
Human
(5)
Primate
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-1919 Applications Species
Vukotic M, Nolte H, Konig T et al. Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria Mol. Cell 2017 Jul 08 [PMID: 28712724] (Human) Human
Diokmetzidou A, Soumaka E, Kloukina I et al. Desmin and aB-crystallin interplay in maintenance of mitochondrial homeostasis and cardiomyocyte survival. J. Cell. Sci. Aug 26 2016 [PMID: 27566162] (WB) WB
Wrobel L, Sokol AM, Chojnacka M, Chacinska A The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly. Sci Rep 2016 Jun 06 [PMID: 27265872] (WB) WB
Nguyen NT, Now H, Kim WJ et al. Ubiquitin-like modifier FAT10 attenuates RIG-I mediated antiviral signaling by segregating activated RIG-I from its signaling platform Sci Rep Mar 21 2016 [PMID: 26996158] (WB, Human) WB Human
Li L, Gao G, Shankar J et al. p38 MAP Kinase-dependent phosphorylation of the Gp78 E3 ubiquitin ligase controls ER-mitochondria association and mitochondria motility. Mol. Biol. Cell 2015 Sep 02 [PMID: 26337390] (ICC/IF, Primate) ICC/IF Primate
Park SJ, Jeong J, Park YU et al. Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics Sci Rep 2015 Mar 03 [PMID: 25732993] (WB, Human) WB Human
Catalina-Rodriguez O, Kolukula VK, Tomita Y et al. The mitochondrial citrate transporter, CIC, is essential for mitochondrial homeostasis. Oncotarget 2012 Oct [PMID: 23100451]
Yang RF, Zhao GW, Liang ST et al. Mitofilin regulates cytochrome c release during apoptosis by controlling mitochondrial cristae remodeling Biochem Biophys Res Commun 2012 Oct 8 [PMID: 23058921] (WB, Human) WB Human
Odgren, PR et al. Molecular characterization of mitofilin (HMP), a mitochondria-associated protein with predicted coiled coil and intermembrane space targeting domains. J Cell Sci;109 ( Pt 9):2253-64. 1996 Sep. [PMID: 8886976] (WB, ICC/IF, Human) WB, ICC/IF Human

Reviews for Mitofilin Antibody (NB100-1919) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Rat.
We have 3 reviews tested in 3 application: Simple Western, WB, IP.

Reviews using NB100-1919:
Filter by Applications
Simple Western
(1)
WB
(1)
IP
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Mitofilin NB100-1919
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reviewed by:
Julian Wong
Simple Western Rat 08/18/2017
View

Summary

ApplicationSimple Western
Sample Testedcell line, whole cell lysate
SpeciesRat
LotC-1
Western Blot Mitofilin NB100-1919
Enlarge
reviewed by:
Kathleen Emanuel
WB Human 06/14/2016
View

Summary

ApplicationWestern Blot
Sample TestedProtein lysate from mitochondria isolated from SH-SY5Y cells
SpeciesHuman
LotB-1

Blocking

Blocking DetailsSuperBlock : 15 minutes : Room Temperature

Primary Anitbody

Dilution Ratio1:1000, 4 degree C, Overnight, 1:8 Superblock: TBST

Secondary Antibody

Secondary DescriptionLicor IR Dye 800 Goat anti-rabbit
Secondary Manufacturer Cat#(Product # 926-32211)
Secondary Concentration1:20000

Details

Detection NotesLicor Odyssey, Fluorescent labeling (No exposure needed)
Immunoprecipitation Mitofilin NB100-1919
Enlarge
reviewed by:
Antigoni Diokmetzidou
IP Mouse 08/27/2013
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Summary

ApplicationImmunoprecipitation
Sample TestedMouse heart
SpeciesMouse
LotA4
Comments3ul of the anti-mitofilin antibody was added to 8mg total heart lysate and incubated o/n at 4C

Blocking

Blocking Details5% milk in PBS

Primary Anitbody

Dilution Ratioo/n at 4C in 5% milk in 1xPBS

Secondary Antibody

Secondary Descriptionanti-goat HRP
Secondary Manufacturer Cat#Sigma
Secondary Concentration1:50000

Details

Detection NotesECL reagent, x-ray blue films
Immunoprecipitation Details100ul slurry protein A/G, 3h RT, 6x washes

Comments

Comments3ul of the anti-mitofilin antibody was added to 8mg total heart lysate and incubated o/n at 4C

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mitofilin Antibody (NB100-1919). (Showing 1 - 1 of 1 FAQs).

  1. One of our customers is interested in testing your Anti-Mitofilin antibody (NB100-1919) with IHC. Do you know how likely this Ab will be in succeeding to this attempt?
    • This Ab has been validated for use in IHC-P in Canine, Feline, Mouse, Rat and Human.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Mitofilin Antibody (NB100-1919)

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Research Areas for Mitofilin Antibody (NB100-1919)

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Blogs on Mitofilin.

Mitofilin and the Mitochondrial Inner Membrane Organizing System (MINOS)
Mitofilin was originally described as a heart muscle protein due to its high expression in the heart. Recently, analysis of the human heart mitochondrial proteome demonstrated that Mitofilin is one of the most abundant mitochondrial proteins (1). Rese...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Julian Wong
08/18/2017
Application: Simple Western
Species: Rat

Kathleen Emanuel
06/14/2016
Application: WB
Species: Human

Antigoni Diokmetzidou
08/27/2013
Application: IP
Species: Mouse

Bioinformatics

Gene Symbol IMMT
Entrez
Uniprot