MITF Antibody (C5) - BSA Free

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clone: C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MITF Antibody (C5) - BSA Free Summary

• Description: Novus Biologicals Mouse MITF Antibody (C5) - BSA Free (NB110-10872) is a monoclonal antibody validated for use in IHC, WB and IP. Anti-MITF Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: N-terminal fragment of human microphthalmia protein.
• Localization: Nuclear
• Specificity: Microphthalmia (C5) this antibody recognizes the 52/56 kD band and not the longer 60-70 kD proteins. Mouse monoclonal clone C5 anti-Microphthalmia antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia from human, mouse or rat.
• Protein/Peptide Type: Animal-Free Recombinant Proteins
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MITF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Immunoprecipitation 2 ug/mg protein lysate
  • Western Blot 1:500
• Application Notes: IHC-P: Antigen retrieval is not essential but may optimise staining. WB: Detects a band of approximately 52-56 kDa. The antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia.
• Theoretical MW: 52-56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.08% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for MITF Antibody (C5) - BSA Free

• bHLHe32
• bHLHe32MI
• Class E basic helix-loop-helix protein 32
• MI
• microphthalmia-associated transcription factor
• MITF
• Waardenburg syndrome, type 2A
• WS2A

Background

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MITF Antibody (NB110-10872)(2)

Publications using NB110-10872

• Emmons MF, Bennett RL, Riva A et al. HDAC8-mediated inhibition of EP300 drives a transcriptional state that increases melanoma brain metastasis Nature communications 2023-11-29 [PMID: 38030596] (WB, Human)
• Xu Z, Li Y, Wang D et al. Mutated SASH1 promotes Mitf expression in a heterozygous mutated SASH1 knock in mouse model Int. J. Mol. Med. 2020-06-19 [PMID: 32582980] (WB, IF/IHC, Mouse)

FAQs for MITF Antibody (NB110-10872). (Showing 1 - 1 of 1 FAQs).

1. Has this antibody been used in ChIP application?
   • We have not yet validated our MiTF antibody with catalogue number NB110-10872 for ChIP in house. We have however tested it for Western blotting, immunoprecipitation, and IHC with paraffin-embedded tissue samples.

Bioinformatics

• Gene Symbol: MITF
• Entrez:
  • 4286(Human)
  • 17342(Mouse)
  • 25094(Rat)
• Uniprot:
  • O75030(Human)