MIS/AMH Antibody

Product Discontinued

MIS/AMH Antibody Summary

• Immunogen: E. coli-derived recombinant rat MIS/AMH
  Lys447-Arg553
  Accession # P49000
• Specificity: Detects mouse and rat MIS/AMH in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human MIS is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AMH
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIS/AMH Antibody

• AMH
• MIF
• MIS
• Muellerian hormone
• muellerian-inhibiting factor
• muellerian-inhibiting substance
• Mullerian hormone
• Mullerian inhibiting factor
• Mullerian inhibiting substance

Background

MIS, also known as anti-Müllarian hormone (AMH), belongs to the TGF-beta superfamily. It is produced by the fetal testis and plays a central role in male sex differentiation. Postnatally, MIS is expressed in both the testis and the ovary, where it also has a regulatory role in gonadal functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIS/AMH Antibody (AF1446)(4)

Publications using AF1446

• S Bagheri-Fa, H Chen, S Wilson, K Ayers, J Hughes, F Sloan-Bena, P Calvel, G Robevska, B Puisac, K Kusz-Zamel, S Gimelli, A Spik, J Jaruzelska, A Warenik-Sz, S Faradz, S Nef, J Pié, P Thomas, A Sinclair, D Wilhelm The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice PLoS ONE, 2020-01-07;15(1):e0227411. 2020-01-07 [PMID: 31910233] (IF, Mouse)
• Long-Term Moderate Oxidative Stress Decreased Ovarian Reproductive Function by Reducing Follicle Quality and Progesterone Production PLoS ONE, 2016-09-27;11(9):e0162194. 2016-09-27 [PMID: 27676390] (Western Blot, Mouse)
• Nilsson EE, Schindler R, Savenkova MI, Skinner MK Inhibitory actions of Anti-Mullerian Hormone (AMH) on ovarian primordial follicle assembly. PLoS ONE, 2011-05-27;6(5):e20087. 2011-05-27 [PMID: 21637711] (IHC-P, Rat)
• Wang PY, Protheroe A, Clarkson AN, Imhoff F, Koishi K, McLennan IS Mullerian inhibiting substance contributes to sex-linked biases in the brain and behavior. Proc. Natl. Acad. Sci. U.S.A., 2009-04-09;106(17):7203-8. 2009-04-09 [PMID: 19359476] (IHC, Mouse)

Bioinformatics

• Gene Symbol: AMH
• Uniprot:
  • P49000()