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FAQs for MIS/AMH Antibody (NBP2-11800). (Showing 1 - 2 of 2 FAQ).
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We are working on a Sandwich ELISA platform for AMH assay. We have the following queries regarding the AMH antibodies: 1. Do you recommend any pairs of the antibodies for Sandwich Elisa platform for Human AMH protein detection? 2. Were the antibodies raised against recombinant protein or synthetic peptide or Native AMH protein? 3. What was the molecular weight of the AMH protein (140KDa or 22KDa) used to check the specificity of the antibodies?
- In regards to your sandwich ELISA inquiry, I was able to find a potential pair of antibodies that may work for your experiment (catalog numbers NBP1-70639 and NBP2-11800). Neither of these antibodies has been tested in ELISA or as a pair, so we cannot guarantee them in this application. The monoclonal antibody was raised against the human recombinant AMH protein. The polyclonal was raised against an AMH peptide. Since we do not epitope map the monoclonal, we cannot say for certain that it will not bind to a similar region as the polyclonal. If you would like to test these in ELISA, then I can recommend our Innovator's Reward program. The theoretical and observed molecular weight for this protein is 59kDa. You can find more information on UniProt about this protein: UniProt P03971.
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Was the entire sequence of the immunogen used (the entire protein) or a region?
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