MIS/AMH Antibody (213828) [Unconjugated]


Recombinant Human MIS/AMH protein was serially diluted 2-fold and captured by Mouse Anti-Human MIS/AMH Monoclonal Antibody (Catalog # MAB17374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse ...read more

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Reactivity HuSpecies Glossary
Applications ELISA

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MIS/AMH Antibody (213828) [Unconjugated] Summary

E. coli-derived recombinant human MIS/AMH
Accession # NP_000470
Detects human MIS/AMH in direct ELISAs.
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Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIS/AMH Antibody (213828) [Unconjugated]

  • AMH
  • MIF
  • MIS
  • Muellerian hormone
  • muellerian-inhibiting factor
  • muellerian-inhibiting substance
  • Mullerian hormone
  • Mullerian inhibiting factor
  • Mullerian inhibiting substance


Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary (1). The human MIS gene encodes a 553 amino acid residue (aa) prepropeptide containing a signal a sequence (1‑24), a pro-region (25‑455), and the carboxyl-terminal bioactive protein (446-553) (2-4). MIS is synthesized and secreted as a disulfide-linked homodimeric pro-protein. Proteolytic cleavage is required to generate the N-terminal pro-region and the C-terminal bioactive protein, which remain associated in a non-covalent complex. Recombinant C-terminal MIS has been shown to be bioactive. However, the complex with the N-terminal pro-region showed enhanced activity (3, 5). The C-terminal region contains the seven canonical cysteine residues found in TGF-beta  superfamily members. These cysteine residues are involved in inter- and intra-molecular disulfide bonds, which forms the cysteine knot structure. Human and mouse MIS share 73% and 90% aa sequence identity in their pro-region and C-terminal region, respectively. MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo (6). Posnatally, MIS has been shown to regulate gonadal functions (1). MIS inhibits Leydig cell proliferation and is a regulator of the initial and cyclic recruitment of ovarian follicles. MIS has also been found to have anti-proliferative effects on breast, ovarian and prostate tumor cells (7-9).

Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, have been implicated in MIS signaling (10-12). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members. Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad 8 (10-12).

  1. Teixeira, et al. (2001) Endocrine Rev. 22:657.
  2. Pepinsky, R.et al. (1988) J. Biol. Chem. 263:18961.
  3. Wilson, C.A. et al. (1993) Mol. Endocrinol. 7:247.
  4. Kurian, M.S. et al. (1995) Clin. Cancer Res. 1:343.
  5. Nachtigal, J.S. and H.A. Ingraham (1996) Proc. Natl. Acad. Sci. USA 93:7711.
  6. MacLaughlin, D.T. et al. (1991) Methods Enzymol. 35:358.
  7. Laurich, V.M. et al. (2002) Endocrinology 143:3351.
  8. McGee, E.A. et al. (2001) Biol. Reprod. 64:293.
  9. Segev, D.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:239.
  10. Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
  11. Clarke, T.R. et al. (2001) Mol. Endocrinol. 15:946.
  12. Visser, J.A. (2003) Mol. Cell. Endocrinol. 211:65.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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