MIS/AMH Antibody (213828) [Unconjugated]

Images

 
Recombinant Human MIS/AMH protein was serially diluted 2-fold and captured by Mouse Anti-Human MIS/AMH Monoclonal Antibody (Catalog # MAB17374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
213828
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

MIS/AMH Antibody (213828) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human MIS/AMH
Ala453-Arg560
Accession # NP_000470
Specificity
Detects human MIS/AMH in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIS/AMH Antibody (213828) [Unconjugated]

  • AMH
  • MIF
  • MIS
  • Muellerian hormone
  • muellerian-inhibiting factor
  • muellerian-inhibiting substance
  • Mullerian hormone
  • Mullerian inhibiting factor
  • Mullerian inhibiting substance

Background

Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary (1). The human MIS gene encodes a 553 amino acid residue (aa) prepropeptide containing a signal a sequence (1‑24), a pro-region (25‑455), and the carboxyl-terminal bioactive protein (446-553) (2-4). MIS is synthesized and secreted as a disulfide-linked homodimeric pro-protein. Proteolytic cleavage is required to generate the N-terminal pro-region and the C-terminal bioactive protein, which remain associated in a non-covalent complex. Recombinant C-terminal MIS has been shown to be bioactive. However, the complex with the N-terminal pro-region showed enhanced activity (3, 5). The C-terminal region contains the seven canonical cysteine residues found in TGF-beta  superfamily members. These cysteine residues are involved in inter- and intra-molecular disulfide bonds, which forms the cysteine knot structure. Human and mouse MIS share 73% and 90% aa sequence identity in their pro-region and C-terminal region, respectively. MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo (6). Posnatally, MIS has been shown to regulate gonadal functions (1). MIS inhibits Leydig cell proliferation and is a regulator of the initial and cyclic recruitment of ovarian follicles. MIS has also been found to have anti-proliferative effects on breast, ovarian and prostate tumor cells (7-9).

Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, have been implicated in MIS signaling (10-12). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members. Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad 8 (10-12).

  1. Teixeira, et al. (2001) Endocrine Rev. 22:657.
  2. Pepinsky, R.et al. (1988) J. Biol. Chem. 263:18961.
  3. Wilson, C.A. et al. (1993) Mol. Endocrinol. 7:247.
  4. Kurian, M.S. et al. (1995) Clin. Cancer Res. 1:343.
  5. Nachtigal, J.S. and H.A. Ingraham (1996) Proc. Natl. Acad. Sci. USA 93:7711.
  6. MacLaughlin, D.T. et al. (1991) Methods Enzymol. 35:358.
  7. Laurich, V.M. et al. (2002) Endocrinology 143:3351.
  8. McGee, E.A. et al. (2001) Biol. Reprod. 64:293.
  9. Segev, D.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:239.
  10. Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
  11. Clarke, T.R. et al. (2001) Mol. Endocrinol. 15:946.
  12. Visser, J.A. (2003) Mol. Cell. Endocrinol. 211:65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30475
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
7270-IL/CF
Species: Hu
NBP1-52823
Species: Hu, Rt, Po, Bv, Ca, Eq, GP, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-38770
Species: Hu, Mu
Applications: WB
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56603
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
NB100-1596
Species: Hu, Rt, Pm, Rb
Applications: WB, Simple Western, ICC/IF, IHC
NB100-1985
Species: Hu, Mu(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NB100-1533
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-37463
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
AF3075
Species: Hu
Applications: WB, Simple Western, ICC

Publications for MIS/AMH Antibody (MAB17374) (0)

There are no publications for MIS/AMH Antibody (MAB17374).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MIS/AMH Antibody (MAB17374) (0)

There are no reviews for MIS/AMH Antibody (MAB17374). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MIS/AMH Antibody (MAB17374) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional MIS/AMH Products

Diseases for MIS/AMH Antibody (MAB17374)

Discover more about diseases related to MIS/AMH Antibody (MAB17374).
 

Pathways for MIS/AMH Antibody (MAB17374)

View related products by pathway.

PTMs for MIS/AMH Antibody (MAB17374)

Learn more about PTMs related to MIS/AMH Antibody (MAB17374).

Blogs on MIS/AMH

There are no specific blogs for MIS/AMH, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MIS/AMH Antibody (213828) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Uniprot