Mimecan/OIF Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Mimecan/OIF Antibody [NBP1-87766] - Staining of human cell line ASC TERT1 shows localization to endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Mimecan/OIF Antibody [NBP1-87766] - Staining of human gallbladder shows moderate membranous positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Mimecan/OIF Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SLLEELSLAENQLLKLPVLPPKLTLFNAKYNKIKSRGIKANAFKKLNNLTFLYLDHNALESVPLNLPESLRVIHLQFNNIASITDDTFCKANDTSYIRDRIEEIRLEGNPIVLGKHPNSFI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OGN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Mimecan/OIF Protein (NBP1-87766PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Mimecan/OIF Antibody - BSA Free

• DKFZp586P2421
• KSPG25
• mimecan proteoglycan
• Mimecan
• OGN
• OIF
• OIFOG
• osteoglycin (osteoinductive factor)
• osteoglycin
• Osteoinductive factor
• SLRR3A
• SLRR3Acorneal keratan sulfate proteoglycan

Background

OIF is involved in inducing bone formation with TGGB1 and TGFB2 as well as being an important regulator of left ventricular mass. OIF is thought to have a role in cellular growth regulation. OIF is known to have interactions with CRK, ABL1, SRC, IRF1 and USF1. Studies of OIF have been conducted in relation to cholera, conjunctivitis, interferon, adenoma, pituitary tumor and paratyphoid fever.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mimecan/OIF Antibody (NBP1-87766)(2)

Publications using NBP1-87766

• Rower C, Ziems B, Radtke A et al. Toponostics of invasive ductal breast carcinoma: combination of spatial protein expression imaging and quantitative proteome signature analysis. Int J Clin Exp Pathol 2011-03-31 [PMID: 21738817] (IF/IHC, Human)
• Bolander A, Agnarsdottir M, Stromberg S et al. The protein expression of TRP-1 and galectin-1 in cutaneous malignant melanomas. Cancer Genomics Proteomics 2008-11-01 [PMID: 19287070]

Bioinformatics

• Gene Symbol: OGN