Decorin Antibody [Unconjugated]

Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to intralobular stromal tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemical DCN expression in HSc and site-matched NS from burn patients.(a) Immunohistochemistry using a polyclonal goat anti-human DCN antibody and Alexa Fluor 488 secondary antibody (green fluorescence), and counterstained with DAPI (blue fluorescence) in representative site-matched sections of NS and HSc (scale bar = 50 μm). (b) Relative expression of DCN in matched superficial and deep NS and HSc sections was calculated from fluorescence using ImageJ (mean ± SEM, n = 4 patients, * P < 0.001). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0123054), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemical DCN expression in HSc and site-matched NS from burn patients.(a) Immunohistochemistry using a polyclonal goat anti-human DCN antibody and Alexa Fluor 488 secondary antibody (green fluorescence), and counterstained with DAPI (blue fluorescence) in representative site-matched sections of NS and HSc (scale bar = 50 μm). (b) Relative expression of DCN in matched superficial and deep NS and HSc sections was calculated from fluorescence using ImageJ (mean ± SEM, n = 4 patients, * P < 0.001). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0123054), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Decorin Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
  Gly17-Lys359
  Accession # P07585
• Specificity: Detects human Decorin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DCN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10-20 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Decorin Antibody [Unconjugated]

• Bone proteoglycan II
• CSCD
• DCN
• decorin proteoglycan
• Decorin
• dermatan sulphate proteoglycans II
• DSPG2
• PG40
• PGII
• PG-II
• PGS2
• PG-S2
• proteoglycan core protein
• SLRR1B
• SLRR1BPGS2
• small leucine-rich protein 1B

Background

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10-12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta , and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

1. Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
2. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
3. Danielson, K. et al. (1993) Genomics 15:146.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Decorin Antibody (AF143)(29)

Publications using AF143

• Yang, F;Akhtar, MN;Zhang, D;El-Mayta, R;Shin, J;Dorsey, JF;Zhang, L;Xu, X;Guo, W;Bagley, SJ;Fuchs, SY;Koumenis, C;Lathia, JD;Mitchell, MJ;Gong, Y;Fan, Y; An immunosuppressive vascular niche drives macrophage polarization and immunotherapy resistance in glioblastoma Science advances 2024-03-01 [PMID: 38416830] (Neutralization, Human)
• Blanco-Elices C, Morales-�lvarez C, Chato-Astrain J et al. Development of stromal differentiation patterns in heterotypical models of artificial corneas generated by tissue engineering Frontiers in Bioengineering and Biotechnology 2023-03-23 [PMID: 37034263]
• Blanco-Elices C, Morales-�lvarez C, Chato-Astrain J et al. Development of stromal differentiation patterns in heterotypical models of artificial corneas generated by tissue engineering Frontiers in Bioengineering and Biotechnology 3/23/2023 [PMID: 37034263]
• Vincent R. Knight-Schrijver, Hongorzul Davaapil, Semih Bayraktar, Alexander D. B. Ross, Kazumasa Kanemaru, James Cranley et al. A single-cell comparison of adult and fetal human epicardium defines the age-associated changes in epicardial activity Nature Cardiovascular Research 2022-12-21 [PMID: 36938497]
• Daniella dos Santos Courrol, Cristiane Castilho Fernandes da da Silva, Luan Gavião Prado, Rosa Maria Chura-Chambi, Ligia Morganti, Gisele Oliveira de Souza et al. Leptolysin, a Leptospira secreted metalloprotease of the pappalysin family with broad-spectrum activity Frontiers in Cellular and Infection Microbiology 2022-08-23 [PMID: 36081769]
• D Basak, Z Jamal, A Ghosh, PK Mondal, P Dey Talukd, S Ghosh, B Ghosh Roy, R Ghosh, A Halder, A Chowdhury, GK Dhali, BK Chattopadh, ML Saha, A Basu, S Roy, C Mukherjee, NK Biswas, U Chatterji, S Datta Reciprocal interplay between asporin and decorin: Implications in gastric cancer prognosis PLoS ONE, 2021-08-11;16(8):e0255915. 2021-08-11 [PMID: 34379688] (Co-IP, Western Blot, Human)
• CH Liu, BR Wu, YJ Ho, YH Chu, WC Hsu, TJ Tseng, JP Li, WC Liao CHPF Regulates the Aggressive Phenotypes of Hepatocellular Carcinoma Cells via the Modulation of the Decorin and TGF-&amp;beta Pathways Cancers, 2021-03-12;13(6):. 2021-03-12 [PMID: 33809195] (ICC, Human)
• AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020-07-31;10(1):12912. 2020-07-31 [PMID: 32737331] (Western Blot, Mouse)
• Bram Lesaffer, Elisabeth Verboven, Leen Van Huffel, Iván M. Moya, Leo A. van Grunsven, Isabelle A. Leclercq et al. Comparison of the Opn-CreER and Ck19-CreER Drivers in Bile Ducts of Normal and Injured Mouse Livers Cells 2019-04-25 [PMID: 31027317]
• J Svärd, TH Røst, CEN Sommervoll, C Haugen, OA Gudbrandse, AE Mellgren, E Rødahl, J Fernø, SN Dankel, JV Sagen, G Mellgren Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice Sci Rep, 2019-03-15;9(1):4614. 2019-03-15 [PMID: 30874564] (Western Blot, Mouse)
• AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard Osteoblasts are &quot;educated&quot; by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment Breast Cancer Res., 2019-02-27;21(1):31. 2019-02-27 [PMID: 30813947] (Functional Assay, IHC-P, Human)
• MB Goncalves, Y Wu, E Clarke, J Grist, C Hobbs, D Trigo, J Jack, JPT Corcoran Regulation of myelination by exosome associated retinoic acid release from NG2-positive cells J. Neurosci., 2019-02-13;0(0):. 2019-02-13 [PMID: 30760627] (IHC-P, Rat)
• Menon R, Otto E, Kokoruda A, Zhou J, Zhang Z, Yoon E, Chen Y, Troyanskaya O, Spence J, Kretzler M, Cebrian C Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney. Development, 2018-08-30;145(16):. 2018-08-30 [PMID: 30166318] (IHC, Human)
• AR Yoon, J Hong, CO Yun Adenovirus-mediated decorin expression induces cancer cell death through activation of p53 and mitochondrial apoptosis Oncotarget, 2017-09-08;8(44):76666-76685. 2017-09-08 [PMID: 29100340] (Western Blot, Human)
• Maternal obesity drives functional alterations in uterine NK cells JCI Insight, 2016-07-21;1(11):e85560. 2016-07-21 [PMID: 27699222] (Neutralization, Human)
• Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro Food Nutr Res, 2016-09-23;60(0):32615. 2016-09-23 [PMID: 27667774] (Western Blot, Human)
• Josephina A.N. Meester, Geert Vandeweyer, Isabel Pintelon, Martin Lammens, Lana Van Hoorick, Simon De Belder et al. Loss-of-function mutations in the X-linked biglycan gene cause a severe syndromic form of thoracic aortic aneurysms and dissections Genetics in Medicine 2017-04-01 [PMID: 27632686] (Immunohistochemistry, Human)
• Víctor Carriel, Giuseppe Scionti, Fernando Campos, Olga Roda, Begoña Castro, Maria Cornelissen et al. In vitro characterization of a nanostructured fibrin agarose bio-artificial nerve substitute Journal of Tissue Engineering and Regenerative Medicine 2017-05-01 [PMID: 26177604]
• Kwan P, Ding J, Tredget E MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar. PLoS ONE, 2015-04-02;10(4):e0123054. 2015-04-02 [PMID: 25837671] (IHC-P, Human)
• Leanne M. Mullen, Serena M. Best, Siddhartha Ghose, John Wardale, Neil Rushton, Ruth E. Cameron Bioactive IGF-1 release from collagen–GAG scaffold to enhance cartilage repair in vitro Journal of Materials Science: Materials in Medicine 2015-01-11 [PMID: 25577208]
• Saliba M, Ramalanjaona N, Gulberti S, Bertin-Jung I, Thomas A, Dahbi S, Lopin-Bon C, Jacquinet J, Breton C, Ouzzine M, Fournel-Gigleux S Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors. J Biol Chem, 2015-01-07;290(12):7658-70. 2015-01-07 [PMID: 25568325] (Western Blot, Human)
• Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Invest Ophthalmol Vis Sci, 2013-02-05;54(2):1014-25. 2013-02-05 [PMID: 23322569] (Western Blot, Human)
• Zhang Y, Conrad AH, Conrad GW Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking. J. Biol. Chem., 2011-02-18;286(15):13011-22. 2011-02-18 [PMID: 21335557] (Western Blot, Bovine)
• Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. J. Biol. Chem., 2010-11-12;286(4):2636-47. 2010-11-12 [PMID: 21075844] (Western Blot, Human)
• Ranjzad P, Salem HK, Kingston PA Adenovirus-mediated gene transfer of fibromodulin inhibits neointimal hyperplasia in an organ culture model of human saphenous vein graft disease. Gene Ther., 2009-05-28;16(9):1154-62. 2009-05-28 [PMID: 19474808] (IHC, Human)
• Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway. J. Pathol., 2009-05-01;218(1):131-42. 2009-05-01 [PMID: 19224541] (IHC-Fr, Human)
• Kitaya K, Yasuo T Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium. J. Leukoc. Biol., 2008-12-16;85(3):391-400. 2008-12-16 [PMID: 19088176] (IHC-P, Western Blot, Human)
• Bolton K, Segal D, McMillan J, Jowett J, Heilbronn L, Abberton K, Zimmet P, Chisholm D, Collier G, Walder K Decorin is a secreted protein associated with obesity and type 2 diabetes. Int J Obes (Lond), 2008-04-15;32(7):1113-21. 2008-04-15 [PMID: 18414424] (ELISA Development, Human)
• Lallam-Laroye C, Escartin Q, Zlowodzki AS, Barritault D, Caruelle JP, Baroukh B, Saffar JL, Colombier ML Periodontitis destructions are restored by synthetic glycosaminoglycan mimetic. J Biomed Mater Res A, 2006-12-01;79(3):675-83. 2006-12-01 [PMID: 16832824] (IHC-Fr, Hamster)

Bioinformatics

• Gene Symbol: DCN
• Uniprot:
  • P07585()