Decorin Antibody [Unconjugated]

Images

 
Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by ...read more
Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat ...read more
Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Decorin Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
Gly17-Lys359
Accession # P07585
Specificity
Detects human Decorin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 10-20 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF143 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Decorin Antibody [Unconjugated]

  • Bone proteoglycan II
  • CSCD
  • DCN
  • decorin proteoglycan
  • Decorin
  • dermatan sulphate proteoglycans II
  • DSPG2
  • PG40
  • PGII
  • PG-II
  • PGS2
  • PG-S2
  • proteoglycan core protein
  • SLRR1B
  • SLRR1BPGS2
  • small leucine-rich protein 1B

Background

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10‑12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta , and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

  1. Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
  2. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
  3. Danielson, K. et al. (1993) Genomics 15:146.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Decorin Antibody (AF143)(18)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Hamster.

We have publications tested in 8 applications: ELISA Development, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
Functional Assay
(1)
ICC
(1)
IHC
(1)
IHC-Fr
(2)
IHC-P
(2)
Neutralization
(1)
Western Blot
(9)
All Applications
Filter By Species
Human
(13)
Mouse
(2)
Rat
(1)
Bovine
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF143 Applications Species
CH Liu, BR Wu, YJ Ho, YH Chu, WC Hsu, TJ Tseng, JP Li, WC Liao CHPF Regulates the Aggressive Phenotypes of Hepatocellular Carcinoma Cells via the Modulation of the Decorin and TGF-&beta Pathways Cancers, 2021;13(6):. 2021 [PMID: 33809195] (ICC, Human) ICC Human
AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020;10(1):12912. 2020 [PMID: 32737331] (Western Blot, Mouse) Western Blot Mouse
J Svärd, TH Røst, CEN Sommervoll, C Haugen, OA Gudbrandse, AE Mellgren, E Rødahl, J Fernø, SN Dankel, JV Sagen, G Mellgren Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice Sci Rep, 2019;9(1):4614. 2019 [PMID: 30874564] (Western Blot, Mouse) Western Blot Mouse
AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment Breast Cancer Res., 2019;21(1):31. 2019 [PMID: 30813947] (Functional Assay, Human) Functional Assay Human
MB Goncalves, Y Wu, E Clarke, J Grist, C Hobbs, D Trigo, J Jack, JPT Corcoran Regulation of myelination by exosome associated retinoic acid release from NG2-positive cells J. Neurosci., 2019;0(0):. 2019 [PMID: 30760627] (IHC-P, Rat) IHC-P Rat
AR Yoon, J Hong, CO Yun Adenovirus-mediated decorin expression induces cancer cell death through activation of p53 and mitochondrial apoptosis Oncotarget, 2017;8(44):76666-76685. 2017 [PMID: 29100340] (Western Blot, Human) Western Blot Human
Maternal obesity drives functional alterations in uterine NK cells JCI Insight, 2016;1(11):e85560. 2016 [PMID: 27699222] (Neutralization, Human) Neutralization Human
Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro Food Nutr Res, 2016;60(0):32615. 2016 [PMID: 27667774] (Western Blot, Human) Western Blot Human
Kwan P, Ding J, Tredget E MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar. PLoS ONE, 2015;10(4):e0123054. 2015 [PMID: 25837671] (IHC-P, Human) IHC-P Human
Saliba M, Ramalanjaona N, Gulberti S, Bertin-Jung I, Thomas A, Dahbi S, Lopin-Bon C, Jacquinet J, Breton C, Ouzzine M, Fournel-Gigleux S Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors. J Biol Chem, 2015;290(12):7658-70. 2015 [PMID: 25568325] (Western Blot, Human) Western Blot Human
Show All 18 Publications.

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Bioinformatics

Gene Symbol DCN
Uniprot