Decorin Antibody [Unconjugated]

Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to intralobular stromal tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Decorin Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
  Gly17-Lys359
  Accession # P07585
• Specificity: Detects human Decorin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse Decorin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DCN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 10-20 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Decorin Antibody [Unconjugated]

• Bone proteoglycan II
• CSCD
• DCN
• decorin proteoglycan
• Decorin
• dermatan sulphate proteoglycans II
• DSPG2
• PG40
• PGII
• PG-II
• PGS2
• PG-S2
• proteoglycan core protein
• SLRR1B
• SLRR1BPGS2
• small leucine-rich protein 1B

Background

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10‑12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

1. Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
2. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
3. Danielson, K. et al. (1993) Genomics 15:146.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Decorin Antibody (AF143)(13)

Publications using AF143

• AR Yoon, J Hong, CO Yun Adenovirus-mediated decorin expression induces cancer cell death through activation of p53 and mitochondrial apoptosis Oncotarget, 2017;8(44):76666-76685. 2017 [PMID: 29100340] (WB,, Human)
• Maternal obesity drives functional alterations in uterine NK cells JCI Insight, 2016;1(11):e85560. 2016 [PMID: 27699222] (Neut,, Human)
• Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro Food Nutr Res, 2016;60(0):32615. 2016 [PMID: 27667774] (WB,, Human)
• Kwan P, Ding J, Tredget E MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar. PLoS ONE, 2015;10(4):e0123054. 2015 [PMID: 25837671] (IHC Paraffin-embedded,, Human)
• Saliba M, Ramalanjaona N, Gulberti S, Bertin-Jung I, Thomas A, Dahbi S, Lopin-Bon C, Jacquinet J, Breton C, Ouzzine M, Fournel-Gigleux S Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors. J Biol Chem, 2015;290(12):7658-70. 2015 [PMID: 25568325] (WB,, Human)
• Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Invest Ophthalmol Vis Sci, 2013;54(2):1014-25. 2013 [PMID: 23322569] (WB,, Human)
• Zhang Y, Conrad AH, Conrad GW Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking. J. Biol. Chem., 2011;286(15):13011-22. 2011 [PMID: 21335557] (WB,, Bovine)
• Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. J. Biol. Chem., 2011;286(4):2636-47. 2011 [PMID: 21075844] (WB,, Human)
• Ranjzad P, Salem HK, Kingston PA Adenovirus-mediated gene transfer of fibromodulin inhibits neointimal hyperplasia in an organ culture model of human saphenous vein graft disease. Gene Ther., 2009;16(9):1154-62. 2009 [PMID: 19474808] (IHC,, Human)
• Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway. J. Pathol., 2009;218(1):131-42. 2009 [PMID: 19224541] (IHC Frozen,, Human)
• Kitaya K, Yasuo T Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium. J. Leukoc. Biol., 2009;85(3):391-400. 2009 [PMID: 19088176] (WB,, Human)
• Bolton K, Segal D, McMillan J, Jowett J, Heilbronn L, Abberton K, Zimmet P, Chisholm D, Collier G, Walder K Decorin is a secreted protein associated with obesity and type 2 diabetes. Int J Obes (Lond), 2008;32(7):1113-21. 2008 [PMID: 18414424] (ELISA Development,, Human)
• Lallam-Laroye C, Escartin Q, Zlowodzki AS, Barritault D, Caruelle JP, Baroukh B, Saffar JL, Colombier ML Periodontitis destructions are restored by synthetic glycosaminoglycan mimetic. J Biomed Mater Res A, 2006;79(3):675-83. 2006 [PMID: 16832824] (IHC Frozen,, Hamster)

Bioinformatics

• Gene Symbol: DCN
• Entrez:
  • 1634(Human)
  • 13179(Mouse)
• Uniprot:
  • P07585()