Decorin Antibody [Unconjugated]

Images

 
Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by ...read more
Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat ...read more
Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Decorin Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
Gly17-Lys359
Accession # P07585
Specificity
Detects human Decorin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse Decorin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 10-20 ug/mL
Publications
Read Publications using
AF143 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Decorin Antibody [Unconjugated]

  • Bone proteoglycan II
  • CSCD
  • DCN
  • decorin proteoglycan
  • Decorin
  • dermatan sulphate proteoglycans II
  • DSPG2
  • PG40
  • PGII
  • PG-II
  • PGS2
  • PG-S2
  • proteoglycan core protein
  • SLRR1B
  • SLRR1BPGS2
  • small leucine-rich protein 1B

Background

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10‑12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

  1. Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
  2. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
  3. Danielson, K. et al. (1993) Genomics 15:146.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-84971
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85432
Species: Hu
Applications: IHC, IHC-P
NB100-74350
Species: Hu, Mu, Rt, Po, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF5945
Species: Hu, Mu, Rt
Applications: WB
AF2846
Species: Hu
Applications: WB, IHC
NBP2-58327
Species: Hu
Applications: WB, ICC/IF
AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
NBP1-32899
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF942
Species: Mu
Applications: WB, IHC, CyTOF-ready, ICC, ICFlow
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP1-82648
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-55231
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ze
Applications: WB
NBP1-86687
Species: Hu
Applications: WB, IHC, IHC-P
NB100-64980
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Decorin Antibody (AF143)(16)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Hamster.

We have publications tested in 7 applications: ELISA Development, Functional Assay, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
Functional Assay
(1)
IHC
(1)
IHC-Fr
(2)
IHC-P
(2)
Neutralization
(1)
Western Blot
(8)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
Rat
(1)
Bovine
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF143 Applications Species
J Svärd, TH Røst, CEN Sommervoll, C Haugen, OA Gudbrandse, AE Mellgren, E Rødahl, J Fernø, SN Dankel, JV Sagen, G Mellgren Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice Sci Rep, 2019;9(1):4614. 2019 [PMID: 30874564] (Western Blot, Mouse) Western Blot Mouse
AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment Breast Cancer Res., 2019;21(1):31. 2019 [PMID: 30813947] (Functional Assay, Human) Functional Assay Human
MB Goncalves, Y Wu, E Clarke, J Grist, C Hobbs, D Trigo, J Jack, JPT Corcoran Regulation of myelination by exosome associated retinoic acid release from NG2-positive cells J. Neurosci., 2019;0(0):. 2019 [PMID: 30760627] (IHC-P, Rat) IHC-P Rat
AR Yoon, J Hong, CO Yun Adenovirus-mediated decorin expression induces cancer cell death through activation of p53 and mitochondrial apoptosis Oncotarget, 2017;8(44):76666-76685. 2017 [PMID: 29100340] (Western Blot, Human) Western Blot Human
Maternal obesity drives functional alterations in uterine NK cells JCI Insight, 2016;1(11):e85560. 2016 [PMID: 27699222] (Neutralization, Human) Neutralization Human
Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro Food Nutr Res, 2016;60(0):32615. 2016 [PMID: 27667774] (Western Blot, Human) Western Blot Human
Kwan P, Ding J, Tredget E MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar. PLoS ONE, 2015;10(4):e0123054. 2015 [PMID: 25837671] (IHC-P, Human) IHC-P Human
Saliba M, Ramalanjaona N, Gulberti S, Bertin-Jung I, Thomas A, Dahbi S, Lopin-Bon C, Jacquinet J, Breton C, Ouzzine M, Fournel-Gigleux S Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors. J Biol Chem, 2015;290(12):7658-70. 2015 [PMID: 25568325] (Western Blot, Human) Western Blot Human
Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Invest Ophthalmol Vis Sci, 2013;54(2):1014-25. 2013 [PMID: 23322569] (Western Blot, Human) Western Blot Human
Zhang Y, Conrad AH, Conrad GW Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking. J. Biol. Chem., 2011;286(15):13011-22. 2011 [PMID: 21335557] (Western Blot, Bovine) Western Blot Bovine
Show All 16 Publications.

Reviews for Decorin Antibody (AF143) (0)

There are no reviews for Decorin Antibody (AF143). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Decorin Antibody (AF143) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF143

Additional Decorin Products

Bioinformatics Tool for Decorin Antibody (AF143)

Discover related pathways, diseases and genes to Decorin Antibody (AF143). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Decorin Antibody (AF143)

Discover more about diseases related to Decorin Antibody (AF143).
 

Pathways for Decorin Antibody (AF143)

View related products by pathway.

PTMs for Decorin Antibody (AF143)

Learn more about PTMs related to Decorin Antibody (AF143).

Blogs on Decorin

There are no specific blogs for Decorin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Decorin Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol DCN
Uniprot