Decorin Antibody [Unconjugated]

Images

 
Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by ...read more
Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat ...read more
Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Decorin Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
Gly17-Lys359
Accession # P07585
Specificity
Detects human Decorin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse Decorin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 10-20 ug/mL
Publications
Read Publications using
AF143 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Decorin Antibody [Unconjugated]

  • Bone proteoglycan II
  • CSCD
  • DCN
  • decorin proteoglycan
  • Decorin
  • dermatan sulphate proteoglycans II
  • DSPG2
  • PG40
  • PGII
  • PG-II
  • PGS2
  • PG-S2
  • proteoglycan core protein
  • SLRR1B
  • SLRR1BPGS2
  • small leucine-rich protein 1B

Background

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10‑12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Decorin Antibody (AF143)(12)

We have publications tested in 3 confirmed species: Human, Bovine, Hamster.

We have publications tested in 6 applications: ELISA Development, IHC, IHC Frozen, IHC Paraffin-embedded, Neut, WB.


Filter By Application
ELISA Development
(1)
IHC
(1)
IHC Frozen
(2)
IHC Paraffin-embedded
(1)
Neut
(1)
WB
(6)
All Applications
Filter By Species
Human
(10)
Bovine
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF143 Applications Species
Maternal obesity drives functional alterations in uterine NK cells JCI Insight, 2016;1(11):e85560. 2016 [PMID: 27699222] (Neut,, Human) Neut Human
Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro Food Nutr Res, 2016;60(0):32615. 2016 [PMID: 27667774] (WB,, Human) WB Human
Kwan P, Ding J, Tredget E MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar. PLoS ONE, 2015;10(4):e0123054. 2015 [PMID: 25837671] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Saliba M, Ramalanjaona N, Gulberti S, Bertin-Jung I, Thomas A, Dahbi S, Lopin-Bon C, Jacquinet J, Breton C, Ouzzine M, Fournel-Gigleux S Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors. J Biol Chem, 2015;290(12):7658-70. 2015 [PMID: 25568325] (WB,, Human) WB Human
Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Invest Ophthalmol Vis Sci, 2013;54(2):1014-25. 2013 [PMID: 23322569] (WB,, Human) WB Human
Zhang Y, Conrad AH, Conrad GW Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking. J. Biol. Chem., 2011;286(15):13011-22. 2011 [PMID: 21335557] (WB,, Bovine) WB Bovine
Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. J. Biol. Chem., 2011;286(4):2636-47. 2011 [PMID: 21075844] (WB,, Human) WB Human
Ranjzad P, Salem HK, Kingston PA Adenovirus-mediated gene transfer of fibromodulin inhibits neointimal hyperplasia in an organ culture model of human saphenous vein graft disease. Gene Ther., 2009;16(9):1154-62. 2009 [PMID: 19474808] (IHC,, Human) IHC Human
Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway. J. Pathol., 2009;218(1):131-42. 2009 [PMID: 19224541] (IHC Frozen,, Human) IHC Frozen Human
Kitaya K, Yasuo T Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium. J. Leukoc. Biol., 2009;85(3):391-400. 2009 [PMID: 19088176] (WB,, Human) WB Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol DCN
Entrez
Uniprot