MIF Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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MIF Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human MIF (R&D Systems, Catalog # 289-MF)
Pro2-Ala115
Accession # AAA36315
Specificity
Detects human and mouse MIF in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MIF
Purity
Antigen Affinity-purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Readout System
Publications
Read Publications using
BAF289 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIF Antibody [Biotin]

  • EC 5.3.2.1
  • EC 5.3.3.12
  • GIFmacrophage migration inhibitory factor
  • GLIF
  • Glycosylation-inhibiting factor
  • L-dopachrome isomerase
  • L-dopachrome tautomerase
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • MIF
  • MMIF
  • Phenylpyruvate tautomerase

Background

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively.

  1. Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
  2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
  3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
  4. Kozak, C.A. et al. (1995) Genomics 27:405.
  5. Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
  6. Paralkar, V. and G. Wistow (1994) Genomics 19:48.
  7. Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
  8. Flieger, O. et al. (2003) FEBS Lett. 551:78.
  9. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
  10. Sun, H-W. et al. (1996) Protein Eng. 9:631.
  11. Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
  12. Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
  13. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
  14. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
  15. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  16. Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow

Publications for MIF Antibody (BAF289)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA Development, ELISA Development (Detection), Luminex Development.


Filter By Application
ELISA Development
(5)
ELISA Development (Detection)
(1)
Luminex Development
(1)
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Filter By Species
Human
(7)
Mouse
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using BAF289 Applications Species
F Ietta, EAV Ferro, E Bevilacqua, L Benincasa, E Maioli, L Paulesu Role of the Macrophage Migration Inhibitory Factor (MIF) in the survival of first trimester human placenta under induced stress conditions Sci Rep, 2018;8(1):12150. 2018 [PMID: 30108299] (ELISA Development, Human) ELISA Development Human
Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus Sci Rep, 2016;6(0):29909. 2016 [PMID: 27453287] (Human) Human
Kim B, Rongisch R, Hager S, Grieb G, Nourbakhsh M, Rennekampff H, Bucala R, Bernhagen J, Pallua N Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation. PLoS ONE, 2015;10(9):e0137366. 2015 [PMID: 26348853] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010;56(0):1320. 2010 [PMID: 20530731] (Luminex Development, Human) Luminex Development Human
Bruhn A, Verdant C, Vercruysse V, Su F, Vray B, Vincent JL Effects of dexamethasone on macrophage migration inhibitory factor production in sepsis. Shock, 2006;26(2):169-73. 2006 [PMID: 16878025] (ELISA Development, Mouse) ELISA Development Mouse
Matsumoto K, Maruyama N, Maruyama T, Ohnishi Y, Nonaka S, Inoshita A, Ito K, Kitajima S, Abe M, Satomura A, Fujita T Elevated macrophage migration inhibitory factor (MIF) levels in the urine of patients with focal glomerular sclerosis. Clin. Exp. Immunol., 2005;139(2):338-47. 2005 [PMID: 15654833] (ELISA Development, Human) ELISA Development Human
Ashcroft GS, Mills SJ, Lei K, Gibbons L, Jeong MJ, Taniguchi M, Burow M, Horan MA, Wahl SM, Nakayama T Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor. J. Clin. Invest., 2003;111(9):1309-18. 2003 [PMID: 12727922] (ELISA Development, Mouse) ELISA Development Mouse
Matsumoto K, 2019, Kanmatsuse K Urinary levels of macrophage migration inhibitory factor in patients with IgA nephropathy. 7587, 2002;92(2):309-15. 2002 [PMID: 12218308] (ELISA Development, Human) ELISA Development Human
Wang L, Das H, Kamath A, Li L, Bukowski JF Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production. J. Immunol., 2002;168(10):4889-96. 2002 [PMID: 11994438] (Human) Human

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Secondary Antibodies

 

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Biotin BAF289

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Bioinformatics

Gene Symbol MIF
Uniprot