MIF Antibody (4E4) - BSA Free

Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysate

Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in Balb/3T3 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MIF Antibody (4E4) - BSA Free Summary

• Description: Novus Biologicals Mouse MIF Antibody (4E4) - BSA Free (NBP1-04319) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-MIF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human MIF (1-115 aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MIF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:500-1:2000
• Application Notes: Use in IHC reported in scientific literature (PMID:34514093).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for MIF Antibody (4E4) - BSA Free

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MIF
• MMIF
• Phenylpyruvate tautomerase

Background

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIF Antibody (NBP1-04319)(1)

Publication using NBP1-04319

• Wei Q, Liu B, Ji H Et al. Exosome-mediated transfer of MIF confers temozolomide resistance by regulating TIMP3/PI3K/AKT axis in gliomas Molecular Therapy - Oncolytics 2021-09-01 [PMID: 34514093] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: MIF
• Uniprot:
  • NP_002406(Human)