MIF Antibody (4E4)

Images

 
Western Blot: MIF Antibody (4E4) [NBP1-04319] - The extract of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MIF Antibody (4E4) Summary

Immunogen
Recombinant human MIF (1-114 aa) purified from E. coli
Specificity
Anti-human MIF mAb, clone 4E4, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MIF protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MIF
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MIF Antibody (4E4)

  • EC 5.3.2.1
  • EC 5.3.3.12
  • GIFmacrophage migration inhibitory factor
  • GLIF
  • Glycosylation-inhibiting factor
  • L-dopachrome isomerase
  • L-dopachrome tautomerase
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • MIF
  • MMIF
  • Phenylpyruvate tautomerase

Background

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MIF
Entrez
Uniprot