Mic1 Antibody - Azide and BSA Free

Western Blot: Mic1 Antibody [NBP3-03433] - Analysis of extracts of various cell lines, using Mic1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Immunocytochemistry/Immunofluorescence: Mic1 Antibody [NBP3-03433] - Analysis of L929 cells using Mic1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: Mic1 Antibody [NBP3-03433] - Human colon carcinoma using Mic1 Rabbit pAb at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: Mic1 Antibody [NBP3-03433] - Mouse kidney using Mic1 Rabbit pAb at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: Mic1 Antibody [NBP3-03433] - Rat kidney using Mic1 Rabbit pAb at dilution of 1:100 (40x lens).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Mic1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Mic1 Antibody - Azide and BSA Free (NBP3-03433) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Mic1 (NP_037458.3). MGEEDYYLELCERPVQFEKANPVNCVFFDEANKQVFAVRSGGATGVVVKGPDDRNPISFRMDDKGEVKCIKFSLENKILAVQRTSKTVDFCNFIPDNSQLEYTQECKTKNANILGFCWTSSTEIVFITDQGIEFYQVLPEKRSLKLLKSHNLNVNWYMYCPESAVILLSTTVLENVLQPFHFRAGTMSKLPKFEIELPAAPKSTKPSLSERDIAMATIYGQLYVLFLRHHSRTSNSTGAEVVLYHLPREGACKKMHILKLNRTGKFALNVVDNLVVVHHQDTETSVIFDIKLRGEFDGSV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RMC1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Mic1 Antibody - Azide and BSA Free

• chromosome 18 open reading frame 8
• Colon cancer-associated protein Mic1
• FLJ23453
• HsT2591
• Mic-1
• MIC1colon cancer associated protein Mic1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: RMC1