mGluR8 Antibody

Western Blot: mGluR8 Antibody [NBP2-17278] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mGluR8 antibody [N1], N-term diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: mGluR8 Antibody [NBP2-17278] - DIV9 rat E18 primary cortical neurons cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mGluR8 stained by mGluR8 antibody [N1], N-term diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: mGluR8 Antibody [NBP2-17278] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:500.

Product Discontinued

mGluR8 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human mGluR8. The exact sequence is proprietary.
• Localization: Cell membrane; Multi-pass membrane protein
• Predicted Species: Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRM8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for mGluR8 Antibody

• FLJ41058
• glutamate metabotropic receptor 8
• glutamate receptor, metabotropic 8
• GPRC1H
• GPRC1HMGLUR8GLUR8
• GRM8
• metabotropic glutamate receptor 8
• MGC126724
• mGlu8
• mGluR8

Background

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GRM8
• Uniprot:
  • O00222()