mGluR3 Antibody

Western Blot: mGluR3 Antibody [NBP1-31109] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mGluR3 antibody [C3], C-term (NBP1-31109). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: mGluR3 Antibody [NBP1-31109] - mGluR3 antibody [C3], C-term detects mGluR3 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mGluR3 stained by mGluR3 antibody [C3], C-term (NBP1-31109).Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody.Blue: Fluoroshield with DAPI.

Western Blot: mGluR3 Antibody [NBP1-31109] - (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE, antibody diluted at 1:500.

Product Discontinued

mGluR3 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human mGluR3. The exact sequence is proprietary.
• Localization: Cell membrane, Multi-pass membrane protein
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRM3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for mGluR3 Antibody

• GLUR3
• glutamate receptor, metabotropic 3
• GPRC1C
• GPRC1Cglutamate metabotropic receptor 3
• GRM3
• mGlu3
• mGluR3
• MGLUR3metabotropic glutamate receptor 3

Background

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for mGluR3 Antibody (NBP1-31109)(1)

Publication using NBP1-31109

• Ji Y, Hu B et al. Opposing Roles of Estradiol and Testosterone on Stress-Induced Visceral Hypersensitivity in Rats. J Pain 2018-01-07 [PMID: 29496640] (WB, Rat)

Bioinformatics

• Gene Symbol: GRM3
• Entrez:
  • 2913(Human)
• Uniprot:
  • Q14832()