MGL1/2 (CD301a/b) Antibody

RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse MGL1/2 (CD301a/b) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4297, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

• Reactivity: Mu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MGL1/2 (CD301a/b) Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MGL1
  Gln57-Ser304
  Accession # AAH14811
• Specificity: Detects mouse MGL1 and MGL2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MGL1/2 (CD301a/b) Antibody

• CD8
• Leu2
• MGL1/2
• p32

Background

Mouse MGL1 (macrophage galactose N-acetyl-galactosamine (GalNAc) specific Lectin 1, CD301a), also called ASGP-BP (asialoglycoprotein binding protein), is a 38 kDa type II transmembrane glycoprotein of the C-type lectin family (1). Two MGL proteins are encoded by separate genes in the mouse, but share 91% amino acid (aa) identity in the extracellular domain (ECD) (2). Only one MGL occurs in human and rat, and this is more structurally similar to mouse MGL1 than MGL2. However, mouse MGL1 binds Lewis X, in contrast to human MGL and mouse MGL2 which both bind specifically to terminal GalNAc residues (2). Lewis X is a trisaccharide commonly found on leukocytes and some tumor cells. Both mouse MGL proteins are expressed on immature dendritic cells. Mouse MGL1 and MGL2 are markers for connective tissue macrophages of a type termed alternately activated macrophages. These macrophages are induced by IL-4 that is produced during Th2-mediated inflammatory responses to parasitic infections or allergic airway inflammation (3, 4). Quantitative RT-PCR after helminth infection shows a peak of MGL1 expression at 7 days, while MGL2 shows increasing expression for at least 29 days (3). This, and data from MGL1 knockout mice (5), indicates that MGL1 is critical during the formation of granulation tissue, with MGL2 remaining involved during chronic infection. Mouse MGL1 is synthesized with an N-terminal 35 aa cytoplasmic region, a 21 aa transmembrane segment and a 248 aa ECD. The ECD contains one 129 aa carbohydrate recognition domain (CRD) that shows 78% and 63% aa identity with rat and human MGL, respectively.

1. Sato, M. et al. (1992) J. Biochem. 111:331.
2. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
3. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
4. Sato, K. et al. (2005) Int. Immunol. 17:559.
5. Sato, K. et al. (2005) Blood 106:207.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MGL1/2 (CD301a/b) Antibody (AF4297)(7)

Publications using AF4297

• Yao S, Zhou Z, Wang L et al. Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH iScience 6/1/2023 [PMID: 37456855]
• Y Jiang, Y Tang, C Hoover, Y Kondo, D Huang, D Restagno, B Shao, L Gao, J Michael Mc, M Zhou, R Silasi-Man, S McGee, M Jiang, X Bai, F Lupu, C Ruan, JD Marth, D Wu, Y Han, L Xia Kupffer cell receptor CLEC4F is important for the destruction of desialylated platelets in mice Cell Death and Differentiation, 2021-05-15;0(0):. 2021-05-15 [PMID: 33993195] (Neutralization, Mouse)
• J Chida, H Hara, K Uchiyama, E Takahashi, H Miyata, H Kosako, Y Tomioka, T Ito, H Horiuchi, H Matsuda, H Kido, S Sakaguchi Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice PLoS Pathog., 2020-08-26;16(8):e1008823. 2020-08-26 [PMID: 32845931] (Flow Cytometry, IHC, Western Blot, Mouse)
• SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice J. Immunol, 2017-02-20;0(0):. 2017-02-20 [PMID: 28219886] (Flow Cytometry, IHC, Mouse)
• Stouch A, Zaynagetdinov R, Barham W, Stinnett A, Slaughter J, Yull F, Hoffman H, Blackwell T, Prince L IkappaB kinase activity drives fetal lung macrophage maturation along a non-M1/M2 paradigm. J Immunol, 2014-06-30;193(3):1184-93. 2014-06-30 [PMID: 24981452] (IHC, Mouse)
• Hiroshi Kitamura, Yoshinori Naoe, Shunsuke Kimura, Tomomi Miyamoto, Shiki Okamoto, Chitoku Toda et al. Beneficial effects of Brazilian propolis on type 2 diabetes in ob/ob mice: Possible involvement of immune cells in mesenteric adipose tissue Adipocyte 10/22/2013 [PMID: 24052898]
• Strissel KJ, DeFuria J, Shaul ME, Bennett G, Greenberg AS, Obin MS T-cell recruitment and Th1 polarization in adipose tissue during diet-induced obesity in C57BL/6 mice. Obesity (Silver Spring), 2010-01-28;18(10):1918-25. 2010-01-28 [PMID: 20111012] (Flow Cytometry, Mouse)

Bioinformatics

• Uniprot:
  • AAH14811()